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Staphylococcal Surface Proteins As Vaccine Candidates For The Control Of Staphylococcal Mastitis In Dairy Cows, Jessica Jean Vidlund
Staphylococcal Surface Proteins As Vaccine Candidates For The Control Of Staphylococcal Mastitis In Dairy Cows, Jessica Jean Vidlund
Masters Theses
Bovine mastitis, an inflammation of the mammary gland, is the single most costly disease of dairy cattle. Staphylococci, a major bacteria that cause bovine mastitis, are commonly divided into two major groups: Staphylococcus aureus and non-aureus staphylococci (NAS), previously known as coagulase-negative Staphylococcus species (CNS). Current mastitis control programs are not fully effective against staphylococcal mastitis and antibiotics are not sustainable due to limited success and the emergence of antimicrobial resistant bacteria. Non-antibiotic sustainable control tools, such as effective vaccines, are critically needed. Our lab developed and evaluated Staphylococcus aureus surface associated protein (SASP) and Staphylococcus chromogenes surface associated protein …
The Incidence Of Staphylococcus Aureus Mastitis In Maine Dairy Cattle, Morgan Belvin
The Incidence Of Staphylococcus Aureus Mastitis In Maine Dairy Cattle, Morgan Belvin
Honors College
This project investigated the factors surrounding the incidence of Staphylococcus aureus (SA) mastitis in Maine dairy cattle. This type of mastitis is driven by an antibiotic resistant pathogen that is very contagious among cattle, though farmers are often unaware of how common this type of mastitis is and that it could be present in their herds. My hypothesis was that specific farm factors, like milkers performing other tasks during milking, as well as the act of pre-dipping versus not pre-dipping, would explain the increase in SA mastitis. A thirty-seven-question IRB-approved survey was subsequently distributed to farmers in the University of …
The Effect Of Staphylococcus Aureus Exposure On White-Tailed Deer Trabecular Bone Stiffness And Yield, Emily Brooke Long
The Effect Of Staphylococcus Aureus Exposure On White-Tailed Deer Trabecular Bone Stiffness And Yield, Emily Brooke Long
Graduate Theses
Osteomyelitis is an infection of the bone or bone marrow caused by the infiltration of bacteria, resulting in destructive inflammation, bone necrosis and abnormal bone remolding. With a growing number of osteomyelitis diagnoses, many of which are linked to Staphylococcus aureus (S. aureus), it is imperative to understand the pathology of S. aureus in relation to bone to provide better diagnostics and patient care. While the cellular mechanisms of S. aureus and osteomyelitis have been studied, little information exists on the biomechanical effects of such infections. We postulated that exposure to S. aureus for 72 hours would significantly decrease both …
The Effect Of Staphylococcus Aureus Extracellular Products (Secp) On Performance,Selective Innate Immune Indexes, And Humoral Immune Response Against Avian Influenzaand Newcastle Disease Vaccines In Broiler Chickens, Teimour Tabari, Younes Papi, Esmaeil Azizi, Forough Talazadeh, Ramezan Ali Jafari, Masoud Ghorbanpoor
The Effect Of Staphylococcus Aureus Extracellular Products (Secp) On Performance,Selective Innate Immune Indexes, And Humoral Immune Response Against Avian Influenzaand Newcastle Disease Vaccines In Broiler Chickens, Teimour Tabari, Younes Papi, Esmaeil Azizi, Forough Talazadeh, Ramezan Ali Jafari, Masoud Ghorbanpoor
Turkish Journal of Veterinary & Animal Sciences
Improving the immune response is important for many reasons such as vaccine failure, immunosuppressive diseases, and antibiotics misuse. Research on materials likely to have immune-enhancing effects has been on the rise. Factors, substances, or compounds that can regulate different functions of the immune system can be used to enhance specific and even nonspecific responses to vaccines. Different amounts (0, 25, 50, and 100 μg) of Staphylococcus aureus extracellular products (SECP) were injected subcutaneously to different groups of chickens during the first vaccination against avian influenza (AI) and Newcastle disease (ND) viruses to investigate the effect of SECP on innate and …
Determination Of Growth And Toxin Production Potential Of Staphylococcus Aureus And Clostridium Perfringens During Döner Production Process, Funda Yilmaz Eker, Kami̇l Bostan
Determination Of Growth And Toxin Production Potential Of Staphylococcus Aureus And Clostridium Perfringens During Döner Production Process, Funda Yilmaz Eker, Kami̇l Bostan
Turkish Journal of Veterinary & Animal Sciences
Döner is a traditional meat meal that is commonly consumed in Turkey. Although it receives heat treatment during processing, some microorganisms including pathogens may remain alive. Therefore, this study was carried out to determine the changes in the number of microorganisms (total aerobic mesophilic bacteria, Enterobacteriaceae, Escherichia coli, aerobic mesophilic spore-forming bacteria, Staphylococcus-Micrococcus spp.) and survival potential of some pathogenic bacteria (Staphylococcus aureus and Clostridium perfringens) during the cooking period. Experimentally prepared döner kebabs were cooked continuously or intermittently. This process resulted in a reduction of microorganisms depending on the species and the use of low quality raw materials, and …
Prevalence Of Blaz Gene And Other Virulence Genes In Penicillin-Resistant Staphylococcus Aureus Isolated From Bovine Mastitis Cases In Gansu, China, Feng Yang, Qi Wang, Xurong Wang, Ling Wang, Min Xiao, Xinpu Li, Jinyin Luo, Shidong Zhang, Hongsheng Li
Prevalence Of Blaz Gene And Other Virulence Genes In Penicillin-Resistant Staphylococcus Aureus Isolated From Bovine Mastitis Cases In Gansu, China, Feng Yang, Qi Wang, Xurong Wang, Ling Wang, Min Xiao, Xinpu Li, Jinyin Luo, Shidong Zhang, Hongsheng Li
Turkish Journal of Veterinary & Animal Sciences
Staphylococcus aureus is a major etiological agent of bovine mastitis worldwide. In this study, 37 strains of S. Aureus resistant to penicillin were isolated from bovine mastitis cases in Gansu Province for investigating blaZ and virulence-related genes, including tst, eta, etb, lukPV, lukED, lukM, hla, hlb, hld, and edin. Antibiotic resistance was based on disk diffusion method and blaZ and virulence-associated genes were studied by polymerase chain reaction. Penicillin resistance gene blaZ was detected in 35/37 (94.6%) of penicillin-resistant S. Aureus isolates. tst, lukPV, lukED, hla, hlb, and hld were observed in 5.4%, 2.7%, 89.2%, 70.3%, 73.0%, and 70.3% of …
Serum Pharmacokinetics And Tissue Concentrations Of A Newrecrystallized Enrofloxacin Hydrochloride-Dihydrate In Hamsters, Alma Carrascosa, Alejandro De La Peña, Lilia Gutierrez, Hector Sumano
Serum Pharmacokinetics And Tissue Concentrations Of A Newrecrystallized Enrofloxacin Hydrochloride-Dihydrate In Hamsters, Alma Carrascosa, Alejandro De La Peña, Lilia Gutierrez, Hector Sumano
Turkish Journal of Veterinary & Animal Sciences
Pharmacokinetics (PK), pharmacodynamics (PD), and PK/PD ratios of a new solvate of enrofloxacin (enrofloxacin hydrochloride-dihydrate; enro-C), were studied in hamsters. Enrofloxacin from Baytril1397904493 5% (enroR) served as the reference preparation. Two groups of 60 Syrian golden hamsters were intramuscularly injected individually with 10 mg/kg of enroR or with enro-C. Tissue and serum samples were obtained for 72 h; enrofloxacin concentrations were determined by HPLC with UV detection. Ninety percent minimum inhibitory concentrations (MIC90) were determined for strains of methicillin-resistant Staphylococcus aureus, Leptospira interrogans, and Escherichia coli. All PK variables were statistically different between groups (P < 0.01). CMAX of enrofloxacin for enro-C was 17.3 μg/mL and it was 2.6 μg/mL for enroR. AUC was considerably higher for enro-C (459.2 μg/mL h vs. 19.9 μg/mL h). There were no statistically significant differences in MIC90 values between enroR and enro-C. Tissue concentrations of enro-C in all cases were higher and remained above the MIC for longer periods than those of enroR. Relevant PK/PD ratios for enrofloxacin (AUC/MIC ? 125 and CMAX > MIC = 10-12) are consequently …
Staphylococcus Aureus Growth And Enterotoxin Production In Italian Caciotta Cheese, Francesca Pedonese, Carlo D'Ascenzi, Beatrice Torracca, Clizia Zingoni, Barbara Turchi, Filippo Fratini, Roberta Nuvoloni
Staphylococcus Aureus Growth And Enterotoxin Production In Italian Caciotta Cheese, Francesca Pedonese, Carlo D'Ascenzi, Beatrice Torracca, Clizia Zingoni, Barbara Turchi, Filippo Fratini, Roberta Nuvoloni
Turkish Journal of Veterinary & Animal Sciences
To evaluate growth and enterotoxin production of Staphylococcus aureus in raw milk caciotta, an Italian soft cheese, experimental cheesemaking trials were carried out with and without a commercial starter. Two different inocula of selected enterotoxin-producing S. aureus strains were tested: high (mean value 5.03 log CFU/mL of milk) and low (mean value 3.22 log CFU/mL of milk). Uninoculated cheeses with milk containing 2.15 log CFU/mL mean value of coagulase-positive staphylococci were also examined. With higher inocul a coagulase-positive staphylococci counts reached 7.57 log CFU/g without starter and had significantly higher values after the acidification phase than those with starter (below …
Presence And Antibiotic Susceptibility Patterns Of Contagious Mastitis Agents (Staphylococcus Aureus And Streptococcus Agalactiae) Isolated From Milks Of Dairy Cows With Subclinical Mastitis, Serkan İki̇z, Beren Başaran, Enver Bariş Bi̇ngöl, Ömer Çeti̇n, Güven Kaşikçi, Naci̇ye Yakut Özgür, Meli̇h Uçmak, Özge Yilmaz, Mehmet Can Gündüz, Ahmet Sabuncu
Presence And Antibiotic Susceptibility Patterns Of Contagious Mastitis Agents (Staphylococcus Aureus And Streptococcus Agalactiae) Isolated From Milks Of Dairy Cows With Subclinical Mastitis, Serkan İki̇z, Beren Başaran, Enver Bariş Bi̇ngöl, Ömer Çeti̇n, Güven Kaşikçi, Naci̇ye Yakut Özgür, Meli̇h Uçmak, Özge Yilmaz, Mehmet Can Gündüz, Ahmet Sabuncu
Turkish Journal of Veterinary & Animal Sciences
Mastitis is recognized as one of the most important diseases affecting the dairy industry. The antibiotic susceptibility test is important to achieve accurate treatment in subclinical mastitis. This study was conducted to determine the presence of contagious mastitis agents (Streptococcus agalactiae and Staphylococcus aureus) in 270 bovine milk samples collected from 132 dairy cows with subclinical mastitis in 15 different dairy farms located in the Marmara Region of Turkey. A total of 256 bacterial pathogens were isolated, of which 25 isolates were identified as S. agalactiae and 12 isolates were identified as S. aureus. Antibiotic susceptibilities of the isolates were …
Effects Of Sodium Lactate On The Presence Of Staphylococcus Aureus And Enterotoxins In Çiğ Köfte (Raw Meatball), Enver Bariş Bi̇ngöl, Ömer Çeti̇n, Hi̇lal Çolak Üzüm, Hamparsun Hampi̇kyan
Effects Of Sodium Lactate On The Presence Of Staphylococcus Aureus And Enterotoxins In Çiğ Köfte (Raw Meatball), Enver Bariş Bi̇ngöl, Ömer Çeti̇n, Hi̇lal Çolak Üzüm, Hamparsun Hampi̇kyan
Turkish Journal of Veterinary & Animal Sciences
Çiğ köfte is a traditional raw meat product consumed almost everywhere in Turkey. It has a poor hygienic quality and can contain several pathogenic bacteria as no heating or cooking process is applied during any stage of manufacturing. The effects of sodium lactate on the presence of Staphylococcus aureus were investigated during the display-life of çiğ köfte and the formation of enterotoxins was also investigated. For this purpose, çiğ köfte samples were manufactured with different concentrations of sodium lactate (1%, 2%, 3%, and 4% NaL) and examined either in ambient (20 ± 2 °C) or refrigerator (4 ± 2 °C) …
An Hsi Report: Industrial Farm Animal Production And Livestock Associated Mrsa (Methicillin Resistant Staphylococcus Aureus), Humane Society International
An Hsi Report: Industrial Farm Animal Production And Livestock Associated Mrsa (Methicillin Resistant Staphylococcus Aureus), Humane Society International
HSI REPORTS
Staphylococcus aureus is a leading cause of bacterial infection and is increasingly found to be resistant to antibiotic therapy. A newly described type of Methicillin Resistant Staphylococcus aureus carried by farm animals, Livestock Associated MRSA (LA-MRSA), is now causing infections in humans with and without direct livestock contact. A reduction in the non-therapeutic use of antibiotics in feed would likely reduce the capacity of industrial animal agriculture to continue to create, disseminate, and perpetuate a large reservoir of LA-MRSA on a global scale, but more fundamental changes in the way animals are raised for food may be necessary forestall a …
The Detection Of Classical Enterotoxins Of Staphylococcus Aureus In Raw Cow Milk Using The Elisa Method, Ebrahim Rahimi, Hassan Momtaz, Amir Shakerian, Hamid Reza Kavyani
The Detection Of Classical Enterotoxins Of Staphylococcus Aureus In Raw Cow Milk Using The Elisa Method, Ebrahim Rahimi, Hassan Momtaz, Amir Shakerian, Hamid Reza Kavyani
Turkish Journal of Veterinary & Animal Sciences
Milk and dairy products are frequently contaminated with exterotoxigenic Staphylococcus aureus, which is often involved in Staphylococcal food poisoning. The aim of the present study is to investigate the presence of classical enterotoxins of S. aureus in raw milk that was produced in Isfahan, Iran. For this purpose, 72 milk samples were collected from the bulk milk tanks of 12 milking farms during 3 different seasons (winter, spring, and summer) and tested for the presence of staphylococcal enterotoxins (SEs) using the enzyme-linked immunosorbent assay (ELISA) method. Out of 72 samples studied, 15 samples (20.8%) were positive for at least 1 …
Preliminary Bacterial Study On Subclinical Mastitis And Teat Condition In Dairy Herds Around Shiraz, Masoud Haghkhah, Mohammad Rahim Ahmadi, Hamid Reza Gheisari, Ali Kadivar
Preliminary Bacterial Study On Subclinical Mastitis And Teat Condition In Dairy Herds Around Shiraz, Masoud Haghkhah, Mohammad Rahim Ahmadi, Hamid Reza Gheisari, Ali Kadivar
Turkish Journal of Veterinary & Animal Sciences
Mastitis is a widely occurring and costly disease in the dairy industry. The aim of this study was to isolate bacterial causes of subclinical mastitis and investigate the relationship between subclinical mastitis and teat condition in dairy herds located around Shiraz, Fars province, Iran. From 7 commercial dairy herds around Shiraz 354 lactating cows were selected randomly. Subclinical mastitis was evaluated by the California Mastitis Test (CMT), electrical conductivity (EC), and somatic cell count (SCC). Teat condition was evaluated by measuring teat width and length. In the 68 samples of bacterial cultures identified, 14 different bacterial species were found. Staphylococcus …
A Case Report Of Ulcerative Lymphangitis (A Mini Review Of Causes And Current Therapies), Fereydon Rezazadeh Zavoshti, Amir Babak Sioofy-Khojine, Hassan Ali Mahpeikar
A Case Report Of Ulcerative Lymphangitis (A Mini Review Of Causes And Current Therapies), Fereydon Rezazadeh Zavoshti, Amir Babak Sioofy-Khojine, Hassan Ali Mahpeikar
Turkish Journal of Veterinary & Animal Sciences
Ulcerative lymphangitis, or pigeon fever, is a bacterial infection of the lymphatic vessels of the skin in horses, cattle, sheep, and goats. Corynebacterium pseudotuberculosis is the classical cause of the disease. However, other pathogens such as Staphylococcus aureus have been isolated from cases of ulcerative lymphangitis. The aim of this report is to review the causes of pyoderma and skin abscesses in horses with specific attention to Staphylococcus aureus. A 10-year-old Thoroughbred stallion was examined in the suburbs of Tabriz (northwest of Iran) in February 2007. Skin abscesses were present around the hock joints on the hind limbs, on the …
Advancing Epidemiological Science Through Computational Modeling: A Review With Novel Examples, Scott M. Duke-Sylvester, Eli N. Perencevich, Jon P. Furuno, Leslie A. Real, Holly Gaff
Advancing Epidemiological Science Through Computational Modeling: A Review With Novel Examples, Scott M. Duke-Sylvester, Eli N. Perencevich, Jon P. Furuno, Leslie A. Real, Holly Gaff
Biological Sciences Faculty Publications
Computational models have been successfully applied to a wide variety of research areas including infectious disease epidemiology. Especially for questions that are difficult to examine in other ways, computational models have been used to extend the range of epidemiological issues that can be addressed, advance theoretical understanding of disease processes and help identify specific intervention strategies. We explore each of these contributions to epidemiology research through discussion and examples. We also describe in detail models for raccoon rabies and methicillin-resis-tant Staphylococcus aureus, drawn from our own research, to further illustrate the role of computation in epidemiological modeling.
Coagulase Gene Polymorphism Of Staphylococcus Aureus Isolated From Subclinical Bovine Mastitis, Özkan Aslantaş, Cemi̇l Demi̇r, Hülya Türütoğlu, Zafer Canteki̇n, Yaşar Ergün, Gökhan Doğruer
Coagulase Gene Polymorphism Of Staphylococcus Aureus Isolated From Subclinical Bovine Mastitis, Özkan Aslantaş, Cemi̇l Demi̇r, Hülya Türütoğlu, Zafer Canteki̇n, Yaşar Ergün, Gökhan Doğruer
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the coagulase gene from 80 S. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated that many coagulase gene genotypes are present in the …
Identification And Antimicrobial Susceptibility Of Staphylococcus Aureus And Coagulase Negative Staphylococci From Bovine Mastitis In The Aydın Region Of Turkey, Şükrü Kirkan, Ergün Ö. Göksoy, Osman Kaya
Identification And Antimicrobial Susceptibility Of Staphylococcus Aureus And Coagulase Negative Staphylococci From Bovine Mastitis In The Aydın Region Of Turkey, Şükrü Kirkan, Ergün Ö. Göksoy, Osman Kaya
Turkish Journal of Veterinary & Animal Sciences
Three hundred bovine milk samples with mastitis were used. Eighty-five (28.33%) strains were identified as Staphylococcus aureus whereas 60 (20.00%) strains were isolated as coagulase negative staphylococcus. Bacteriological cultures of coagulase negative staphylococcus were identified as 20 (33.33%) strains of S. hyicus, 16 (26.66%) strains of S. chromogenes, 9 (15.00%) strains of S. epidermidis, 5 (8.33%) strains of S. haemolyticus, 4 (6.66%) strains of S. sciuri, 3 (5.00%) strains of S. lentis and 3 (5.00%) strains of S. cohnii subsp. cohnii. The antibiotic susceptibility test results showed that Staphylococcus aureus strains were susceptible to ciprofloxacin (100.00%), neomycin + bacitracin + …
Efficacy Of Prepartum Intramammary Lactating Cow Treatment In Dairy Heifers, Christopher B. Norman
Efficacy Of Prepartum Intramammary Lactating Cow Treatment In Dairy Heifers, Christopher B. Norman
LSU Master's Theses
Mastitis in prepartum dairy heifers has been recognized as a significant economic problem for the dairy industry. Intramammary infusion of cephapirin sodium was completed in 20 treatment animals and the results were compared to 25 non-treated control animals. Bacteriological data from the quarter milk samples were used to determine and compare initial infection rates and cure rates following calving between treatment and control groups. Comparisons were also made between groups for differences in reproductive performance, milk yield, somatic cell count and milk ketone concentration. Milk yield did not differ between groups for either DHIA monthly average kg/day, 305 day actual …
The Effect Of Different Storage Temperatures On The Growth And Enterotoxin Producing Characteristics Of Stapyhlococcus Aureus In Çiğ Köfte, Emrullah Sağun, Mustafa Ali̇şarli, Hi̇sametti̇n Durmaz
The Effect Of Different Storage Temperatures On The Growth And Enterotoxin Producing Characteristics Of Stapyhlococcus Aureus In Çiğ Köfte, Emrullah Sağun, Mustafa Ali̇şarli, Hi̇sametti̇n Durmaz
Turkish Journal of Veterinary & Animal Sciences
The growing and toxin-producing ability of strains of Staphylococcus aureus producing enterotoxin A (SEA 10652 FDA 196E), B (SEB 10654 FDA 243), C (SEC 10655 137) and D (SED 10656 494) were investigated in çiğ köfte (a raw ball prepared by the hand-kneading of lean ground-beef, fine bulgur and a variety of spices). For this purpose, çiğ köfte samples prepared under experimental conditions (Group I containing enterotoxin A, Group II containing enterotoxin B, Group III containing enterotoxin C, Group IV containing enterotoxin D, and Group V (mix) containing enterotoxins A, B, C, and D) were separately contaminated by strains of …
Investigation Of Staphylococcus Aureus Isolation And Thermonuclease Activity And Enterotoxin Formation In Some Dairy Desserts, Mustafa Ali̇şarli, Yakup Can Sancak, Levent Akkaya, Ci̇hangi̇r Eli̇bol
Investigation Of Staphylococcus Aureus Isolation And Thermonuclease Activity And Enterotoxin Formation In Some Dairy Desserts, Mustafa Ali̇şarli, Yakup Can Sancak, Levent Akkaya, Ci̇hangi̇r Eli̇bol
Turkish Journal of Veterinary & Animal Sciences
In this study, the isolation of Staphylococcus aureus from dairy desserts, and its thermonuclease activity and enterotoxigenic properties were investigated. A total of 175 samples were analyzed consisting of 100 pudding samples (sütlaç [rice pudding] 25, keşkül [milk pudding containing coconut] 30, supangile [chocolate pudding] 25, kazandibi [milk pudding slightly burned on the bottom] 20) and 75 cream pastries (butter-cream 25, chocolate-cream 25, fruit-cream 25). According to the analysis, S. aureus was found in a total of 30 (17%) samples in one sütlaç (4%), three keşkül (10%), four supangile (16%), and two kazandibi (8%) samples (10% of pudding samples; and …