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Evaluation Of Bovine Spermatid Sex Ratio By Fluorescent In Situ Hybridization, Michael Alan Stout
Evaluation Of Bovine Spermatid Sex Ratio By Fluorescent In Situ Hybridization, Michael Alan Stout
LSU Master's Theses
The objective of this study was to use fluorescent in situ hybridization (FISH) to determine if a skew in the sex ratio was present at the level of the bovine round spermatid. Paraffin embedded bovine testicular tissue obtained from biopsies were used to perform FISH, and the seminiferous tubules were examined. Our findings show that the skew does exist within the round spermatids. Analysis of variance on X- and Y-chromosome bearing round spermatids as affected by sex, slide, picture set, and tubule was performed. Sex showed that the variation in round spermatid sex ratio differed between slides (P = 0.0001). …