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Identification Of Candidate Cellulose Utilizing Bacteria From The Rumen Of Beef Cattle, Using Bacterial Community Profiling And Metagenomics, Lee Opdahl
Electronic Theses and Dissertations
The ruminal microbiome allows ruminant animals to convert cellulosic biomass into food products. A majority of ruminal microorganisms remain uncharacterized due, in part, to the complexity of ruminal microbial communities. In order to gain further insight, selection-based batch culturing from bovine rumen fluid, in combination with metagenomics, was used to identify and characterize previously uncharacterized rumen bacteria capable of metabolizing cellulose, which was supplemented as a purified substrate. 16S rRNA-based population analysis was used to identify rumen bacteria enriched within 14 days of culturing. As a result of 4 independent experiments, seven different candidate cellulose-utilizing species-level operational taxonomic units (OTUs) …
Measuring Aquatic Organism Responses To Grassland Restoration: Does The Field Of Dreams Really Exist?, David A. Schumann
Measuring Aquatic Organism Responses To Grassland Restoration: Does The Field Of Dreams Really Exist?, David A. Schumann
Electronic Theses and Dissertations
Landscape homogenization and the degradation of riparian areas has greatly impaired stream ecosystems throughout North America. Conservation programs may repair riparian ecosystems to indirectly improve water quality and instream habitat heterogeneity in hopes to elicit biological responses. However, focused manipulations on isolated stream fragments have rarely achieved biological goals. Prairie streams with restored riparian areas were appraised (chemical, physical, and biological variables) to quantify the indirect effects of prevalent grassland conservation practices on aquatic resources. Riparian rehabilitation, via passive methods, promoted bank-stabilizing vegetation along all conservation stream reaches. Riparian vegetation and function quickly recovered from previous agricultural disturbances at conservation …