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Biophysical Factors Affecting Habitat Suitability For Crassostrea Virginica, Jason D. Tilley

Dissertations

Oyster reefs provide a variety of important ecosystem services. However, the mortality rate of eastern oyster, Crassostrea virginica, the dominant species that produces oyster reefs in the northern Gulf of Mexico, is increasing at an alarming rate due to a variety of abiotic and biological factors. I examined how biophysical factors, including the less-studied fatty acid profiles of the suspended particulate matter on which oysters feed, influenced morphometric condition of C. virginica.

I sampled suspended particulate matter (SPM) and oysters in-situ in the western Mississippi Sound, which historically supported the majority of oyster production in Mississippi waters. Sampling …

Livestock Ticks In The Uae: Prevalence, Distribution, Population Dynamics, And Associated Microorganisms, Nighat Perveen

Dissertations

Ticks are important vectors of an array of viral, bacterial, and protozoan pathogens resulting in a wide range of animal and human diseases in the Middle East and North Africa (MENA). In this study, ticks were collected from camels, cows, sheep, and goats in Abu Dhabi, Dubai, and Sharjah in the United Arab Emirates (UAE). The objectives of the study were to (1) identify tick species of livestock through taxonomic keys and using molecular markers, and determine their prevalence and distribution in the UAE, (2) assess Hyalomma dromedarii seasonal population fluctuation over a year under common camel breeding and management …

Regional Differences In Wild North American River Otter (Lontra Canadensis) Behavior And Communication, Sarah Walkley

Dissertations

This study focuses on the vocalization repertoires of wild North American river otters (Lontra canadensis) in New York and California. Although they are the same species, these two established populations of river otters are separated by a significant distance and are distinct from one another. River otters are semi-aquatic social predators that can be found throughout North America. This is the first study to examine the vocalizations of wild river otters, and results are compared across field sites in the different regions. River otter vocalizations and behaviors in New York were recorded using Bushnell Aggressor trail cameras that …

The Development Of Socio-Sexual Behavior In Beluga Whales (Delphinapterus Leucas), Malin K. Lilley

Dissertations

The reproductive success of the beluga whale is critical for a species facing extinction in its endangered Cook Inlet, Alaska population. To date, little is known about the mating behavior of these whales in wild populations. On the other hand, observations of beluga whales in human care allow researchers to better understand many aspects of their daily lives and life histories that are difficult to assess in wild populations. Thus far, a catalog of socio-sexual behavior has been established based on observations of belugas; however, the developmental trajectory of socio-sexual behavior is not well-understood. The present study explored how socio-sexual …

Bottlenose Dolphin (Tursiops Truncatus) Vocal Responses To Sonar And Spectrally Pink Background Noise, Maria Zapetis

Dissertations

As human populations rise, the level of man-made noise increases globally. Naval sonar and boat traffic are underwater sound sources of particular concern to marine mammal welfare. To better understand the impact of these noise increases on cetaceans, studies can explore animals’ behavioral changes in response to noise. Studies have investigated the ‘dose-response’ relationship between the received sound pressure level of sonar signals and the behavior of cetaceans in the wild, but exposure studies in controlled environments are limited. The studies in this dissertation examined bottlenose dolphin vocal modifications during various experimental noise treatments. Acoustic recordings previously obtained for bottlenose …

Personality In California Sea Lions (Zalophus Californianus) And Harbor Seals (Phoca Vitulina): Methodological Convergence And Species-Specific Emotional Repertoires, Amber De Vere

Dissertations

Despite the wide acceptance of animal personality as a valid area of study, research on marine mammal personality remains remarkably scarce. What literature does exist predominantly focuses on bottlenose dolphins (Frick, 2016; Highfill & Kuczaj, 2007; Kuczaj, Highfill & Byerly, 2012; Lilley, de Vere, Yeatre & Kuczaj, 2018; Moreno, Highfill & Kuczaj, 2017). There is also strong evidence for individual differences in grey seals (Robinson et al., 2015; Twiss & Franklin, 2010; Twiss, Culloch & Pomeroy, 2011; Twiss, Cairns, Culloch, Richards & Pomeroy, 2012), and preliminary research has provided evidence of broad personality factors in pinniped species using behavioral coding …

Interactions And Pathogen Transmission Between Carnivores In Madagascar, Fidisoa Rasambainarivo

Dissertations

Introduced carnivores exert considerable pressure on native predators through predation, competition and disease transmission. Improved understanding of determinant factors of interactions and pathogen transmission between introduced and endemic wildlife may help to predict disease emergence, avoid pathogen spillover and help control outbreaks. Using non-invasive camera traps, I identified areas where transmission of pathogens might happen through records of shared space-use within a protected area in Eastern Madagascar. I showed that indirect interactions between animals were more likely to occur near the research station which may constitute a disease transmission hotspot for carnivores in the landscape. Secondly, I investigated the associations …

Escorting Of Mother-Calf Pairs Of Humpback Whales (Megaptera Novaeangliae) In The Colombian Pacific During The Breeding Season, Natalia Botero Acosta

Dissertations

Humpback whales (Megaptera novaeangliae) belonging to the “breeding G-stock” annually migrate from the Antarctic Peninsula and southern Chile to the southeastern Pacific to reproduce. Associations between mother-calf pairs and escorts were examined in the Gulf of Tribugá, northern Colombian Pacific, using photo-identification and behavioral/spatial sampling. Research hypotheses included: 1. The association between cows and escorts is short-lived, consistent with a male reproductive strategy, 2. The presence of escort(s) elicits a behavioral response from mother-calf pairs and, 3. The patterns of spatial distribution reflect the spatial segregation of maternal females. Groups were classified as mother-calf pairs (Mc), mother, calf …

Evaluating Social Network Dynamics Of Bigg’S Killer Whales (Orcinus Orca) And Vessel Traffic Within A Transboundary Region: Implications For Conservation Management, Courtney Smith

Dissertations

The social lives of animals are defined by group dynamics based on the nature and strength of associations and movements between individuals, often resulting in highly complex and interconnected social networks. However, understanding of how environmental variables may shape this structure is poorly understood. Within the inland waters of Washington State and southern Vancouver Island, British Columbia, mammal-eating Bigg’s (transient) killer whales occur in relatively small, but stable social groups. Group size and occurrence in recent years has increased, coinciding with a growing whale watching industry. Given the central importance of the social network within killer whale population dynamics, such …

Population Structure, Connectivity, And Phylogeography Of Two Balistidae With High Potential For Larval Dispersal: Balistes Capriscus And Balistes Vetula, Luca Antoni

Dissertations

The gray triggerfish (Balistes capriscus) and the queen triggerfish (Balistes vetula) are two exploited reef fish distributed in tropical and temperate shelf waters of the Atlantic Ocean and the Mediterranean Sea. Both species are highly sedentary as adults but disperse pelagic larvae for extended periods of time potentially allowing connectivity across long distances under the action of oceanic currents. In this work population structure, phylogeography, and migration patterns were examined in the two species and contrasted with predictions of larval transport based on surface circulation data. A total of 1,017 gray triggerfish from twelve sampling localities …

Pragmatic Understanding Of Bottlenose Dolphins (Tursiops Truncatus) Use Of A Two-Way Communication System, Pepper Reid Hanna

Dissertations

Pragmatics focuses on how a communication system is used to achieve a communicative goal, the social context of the communication, and the organizational structure of communications (Horn & Ward, 2004; McLaughlin, 1998). There is evidence of pragmatics within animal communication systems. For example, context appears to be an important component in both signal production and a receiver’s response in vervet monkeys (Seyfarth et al. 1980).

MA Thesis: http://aquila.usm.edu/masters_theses/170/

The current study used an underwater keyboard to establish a two-way communication system between humans and dolphins. The purpose of this study was to determine if, under these conditions, dolphins displayed pragmatic …

Efficacy Of Cognitive Enrichment For Bottlenose Dolphins (Tursiops Truncatus): Evaluation Of Planning Abilities Through The Use Of A Novel Problem Solving Task, Lisa Kay Lauderdale

Dissertations

Environmental enrichment is a key component to improving the psychological and physiological well being of animals in human care. Enrichment can be achieved through a variety of modalities, including the addition of objects and scents, or by providing the animals with additional challenges. The effectiveness of specific enrichment should be evaluated on a case-by-case basis to determine if the desired result is achieved. Environmental enrichment devices (EED’s) can be utilized to present novel problems to animals in human care. When confronted with a novel problem, dolphins can plan their behavior to create a more efficient strategy then previously modeled.

The …

A Microbiomic Approach To The Characterization Of The Impacts And Influences Of Viral, Bacterial, And Harmful Algal Bloom Toxins On The Bottlenose Dolphin, Corey David Russo

Dissertations

As apex predators that display high site fidelity Tursiops truncatus (bottlenose dolphin) are indicators of marine ecosystem health. Bottlenose dolphins, additionally, display pathogenesis and immune response similar to that of humans. Humans and coastal bottlenose dolphins, in particular, are constantly exposed to the same industrial, agricultural and domestic toxins and pathogens, contaminants and pollutants. Thus, studies on the bottlenose dolphin are also valuable in bridging the gap between ocean and human health. Bottlenose dolphins are susceptible to viral bacterial and toxin infection. Infection in the bottlenose dolphin manifests itself in the form of mass stranding events, unusual mortality events, chronic …

Effects Of Human Disturbance On Physiology, Behavior, And Ornamentation In The Eastern Bluebird, Lauren Marjorie Gillespie

Dissertations

Overall, few studies have focused on anthropogenic disturbance on wildlife physiology. Research has typically focused on how environmentally contaminated areas or anthropogenic disturbance (e.g. noise, human activity) influences biodiversity, community structure and behavior of individual animals. However, understanding how disturbance influences some aspects of physiology can require sacrifice of the animal, prohibiting ecologically relevant measures of behavior and reproductive success. This research strives to examine covariation between testosterone (T) and corticosterone (CORT), plumage ornamentation, and behavior in two populations of eastern bluebirds (Sialia sialis) that differ in degree to which their habitat is modified by human activity.

In …

Stopover Ecology Of Ruby-Throated Hummingbirds (Archilochus Colubris) During Autumn Migration, Theodore Joseph Zenzal Jr

Dissertations

The Ruby-throated Hummingbird (Archilochus colubris) is one of the smallest Nearctic-Neotropical migrants and the only species of hummingbird that breeds in Eastern North America, yet few studies have investigated the biology of Ruby-throated Hummingbirds when they stopover during migration. Given their small size, high metabolism, aggression, and specialized diet, hummingbirds may represent a migrant that operates on the physiological edge. Therefore it is important to understand the factors that influence their stopover as well as the decision to resume migration. Towards this end, Ruby-throated Hummingbirds were captured during autumn migration along the northern coast of the Gulf of Mexico to …

Clarification Of The Systematics Of The Haploporoidea (Trematoda) With Descriptions Of New Genera And Species, Michael Jay Andres

Dissertations

The superfamily Haploporoidea Nicoll, 1914 comprises two families, the Atractotrematidae Yamaguti, 1939 and the Haploporidae Nicoll, 1914, and the subfamily Cadenatellinae Gibson et Bray, 1982. All members are parasites of the alimentary tract or gall bladder of marine, estuarine, and freshwater herbivorous fishes. Overstreet and Curran (2005a) provided a review of the Haploporidae and recognized four subfamilies, the Chalcinotrematinae Overstreet et Curran, 2005, Haploporinae Nicoll, 1914, Megasoleninae Manter, 1935, and Waretrematinae Srivastava, 1937. In a series of five publications, Blasco-Costa et al. (2009a-e) revised the Haploporinae and erected a fifth subfamily, the Forticulcitinae Blasco-Costa, Balbuena, Kostadinova, et Olson, 2009. The …

Effects Of Animal Management Changes On The Activity Budgets And Walking Rates Of Zoo Elephants, Angela Dawn Mackey

Dissertations

The current study aimed to assess the effects of two management strategies, weekly rototilling of the enclosure and the provision of unpredictable foraging opportunities, on the daily behavior of six elephants housed at the San Diego Zoo. Observations took place from January to June 2013 and were conducted throughout a 24- hour period. The study was divided into three phases, each two months in duration. During the first phase (baseline) no changes were made to the current management routine of the elephants. In Phase 2, rototilling of the elephant enclosures was increased from once monthly to once per week, while …

Revision Of 13 Genera Of Haploporidae (Trematoda), Eric Edward Pulis

Dissertations

The Haploporidae is a family of digeneans united by the combination of the possession of a hermaphroditic sac and a single testis or, rarely, two tandem testes. The major divisions in the Haploporidae have been based on the organization, development, and nature of the male and female reproductive systems. Overstreet and Curran (2005) has been the only attempt to organize the genera of the Haploporidae in a subfamilial framework. In the present work the validity of the subfamily Waretrematinae by Overstreet and Curran (2005) is assessed by morphological and molecular methods, based on original descriptions, type and vouchered, specimens and …

Connecting The Dots: Understanding Migration In The Context Of Other Periods Of The Annual Cycle, Kristina Lee Paxton

Dissertations

Migration is a fundamental characteristic of the life history of many organisms. Large-scale seasonal movements expose migratory species to an array of differing environmental conditions such that the ecology, behavior, and life history strategies of migratory species must balance selection pressures associated with each phase of the annual cycle. Therefore, scientists increasingly realize that to understand the ecology of a migratory species in any one phase of the annual cycle requires an understanding of how other phases may interact with and influence the period of interest. That said, following individuals throughout the year poses a serious challenge.

The focus of …

Population Demographics And Genetics Of Spix's Disk-Winged Bat: Insights Regarding Survival, Mate Choice, Gene Flow And Effective Population Size, Michael Buchalski

Dissertations

Simultaneous study of the demographics and genetics of populations are relatively rare within the literature, despite insights their combined use offers regarding the life history, ecology, and evolution of species. Here I take a comprehensive approach, using capture-recapture data, polymorphic microsatellite markers, and various modeling techniques to examine the demographics and genetics of multiple local populations of Spix’s disk-winged bat (Thyroptera tricolor) in southwestern Costa Rica. T. tricolor is a highly gregarious, neotropical bat species known to form kin-based social groups with high retention of offspring of both sexes. The implications of this highly unusual social structure for …

A Bird In The Binoculars: Understanding Birdwatchers' Potential To Contribute To Sustainable Development And Biological Conservation, Sheri Lynn Glowinski

Dissertations

The three studies comprising this research drew from local economic development and environmental sociology to understand aspects of human dynamics that influence the conservation of birds. Using survey data collected from birdwatchers visiting coastal Alabama, the first study examined the local economic impact of birdwatching tourism and the factors that participants deemed important with respect to their recreation. Birdwatchers spent $103,305 during the four month study period. The total annual economic impact of this recreation on the area was estimated at $937,470, producing a multiplier of 1.48 and the equivalent of 20.3 full time jobs. Infrastructural variables (parking, bathrooms, site …

Tactile Behavior In A Group Of Captive Rough-Toothed Dolphins As A Function Of Opportunities To Play With Objects, Kelly Ann Caffery

Dissertations

Cetaceans live in complex physical and social environments that are frequently changing. In contrast, the captive environment for marine mammals is often lacking in stimulation. As a result, enrichment is often used to increase species-typical behaviors and enhance the well-being of the animals. The purpose of this study was to compare the effects of enrichment objects on the social behavior of a group of seven captive rough-toothed dolphins (Steno bredanensis). Observations of this species in the wild suggest it may have a particular affinity for tactile and object play behaviors. Therefore, these behavior categories were a primary focus …

Investigating The Function Of The Mold Specific Gene M46, In The Pathogenic Dimorphic Fungus Histoplasma Capsulatum, Davida Lashaundra Crossley

Dissertations

Histoplasma capsulatum (Hc) is a dimorphic fungus that is the etiologic agent for the respiratory infection histoplasmosis. The fungus is found in the environment in contaminated soils of birds and bat excreta as a multi-cellular saprophytic mold. Once the soil is disturbed, spores are released and are inhaled into the lungs. In the lungs, the fungus converts to uni-cellular parasitic yeast (Maresca & Kobayashi, 1989). The conversion from mold to yeast is a requirement for pathogenesis. Previously, several mold specific genes have been isolated by our laboratory from a subtracted cDNA library. One such gene, is M46. M46 is a …

Interrelationships Between Testosterone, Aggression, And Parental Care Of A Temperate-Zone, Resident Songbird, The Northern Cardinal (Cardinalis Cardinalis), Margaret Susan Devries

Dissertations

Interrelationships among testosterone (T), aggression, and parental care have received much investigative attention. Within birds, many studies have focused on examining such relationships using species characterized by relatively brief periods of territoriality and breeding. Few have investigated links between circulating T and reproductive behavior in birds that are year-round territorial residents and have lengthy breeding seasons, such as the Northern Cardinal (Cardinalis cardinalis). The following six chapters report and discuss findings from this dissertation’s examination of aspects of T production and potential interconnections with circulating T, aggression, and parental care in male and female cardinals. This work suggests …

Personality Assessment In African Elephants (Loxodonta Africana), Kristina Marie Horback

Dissertations

The following study assessed personality in twelve African elephants using both observational behavior coding and standardized trait rating methods, thus demonstrating consistent individual differences across time and contexts. During the summer of 2010 and 2011, over 640 hours of behavioral data were collected onsite at the San Diego Zoo Safari Park in Escondido, CA. Four coding-based personality traits were determined after analysis: PLAYFUL, CURIOUS, TOLERANT, and, AGGRESSIVE. This data was then compared to survey ratings completed by the animal keeper staff during both summers. Four rating-based personality traits resulted from this analysis: PLAYFUL, CURIOUS, TIMID, and, AGGRESSIVE. All eight composite …

Clam (Corbicula Fluminea) As A Potential Sentinel Of Human Norovirus Contamination In Freshwater, Xunyan Ye

Dissertations

The purpose of this study was to evaluate and validate the use of the clam Corbicula fluminea as a sentinel of human noroviruses (HuNoV) contamination in freshwater. The first specific aim was to develop a new method to extract HuNoV RNA from contaminated bivalves (e.g. oysters, clams) that would be much faster than existing methods. The procedure developed includes an initial total RNA extraction using TRI Reagent, followed by HuNoV RNA concentration and purification using biotinylated probe-capture technology. HuNoV RNA is finally detected by real-time RT-PCR. Using bivalve homogenates spiked with HuNoV, 100 PCR detection units of the virus was …

Genetic Variation In Potentially Virulent Vibrio Parahaemolyticus From The Northern Gulf Of Mexico, Nicholas Felix Noriea Iii

Dissertations

Vibrio parahaemolyticus (V_p) is a gram-negative bacterium found naturally in marine and estuarine environments. V_p is found in oysters including those which are later consumed by the public. Sub-populations of potentially virulent Vp contain specific virulence factors and are relevant human pathogens capable of causing gastroenteritis, wound infection, and death. The tdh and trh genes, both encoding hemolysins, have been correlated with the majority of clinical V_p isolates but have not been shown to be the definitive virulence factors.

A total of 146 V_p isolates from the northern Gulf of Mexico were collected and probed …

Effects Of Hypoxia And 4-Tert-Octylphenol On Gene Expression Profiles Of The Sheepshead Minnow (Cyprinodon Variegatus), Arthur Alan Karels

Dissertations

Hypoxia occurs in estuaries of northern Gulf of Mexico and world-wide, with increasing frequency/severity via eutrophication and anthropogenic influences. Hypoxia inducible factors (HIFs) form transcriptional complex and bind DNA at hypoxia responsive elements (HREs) in promoter regions of genes needed for systemic and cellular adaptation of fish to low dissolved oxygen (hypoxia, DO <2.0 mg/ml). Hypoxia-induced activation of HIF-αs can lead to a cascade of downstream activation, such as erythropoietin (EPO). Return to normal DO levels (normoxia), prolyl hydroxylases (PHDs) are activated to degrade HIF-αs back to baseline. Fish are affected by environmental estrogen mimics, like 4-tert-octylphenol (4tOP), binding estrogen receptor alpha (ERα) at estrogen responsive elements (EREs) and activating genes vitellogenin (VTG). Previous research showed overlap or crosstalk between these two mechanistic pathways. Hypoxia triggers unknown factors regulating ERE-mediated ERα signaling pathway, and stressor combinations could increase/decrease hypoxic or endocrine pathway. Research examined molecular/physiological effects of hypoxia (acute and chronic, moderate and severe) and 4tOP (~60μg/L)on adult male and/or female sheepshead minnow (Cyprinodon variegatus). Three genes identified, cloned, and sequenced (HIF-1α, HIF-2α, and PHD3), plus previously identified genes EPO and VTG, were examined in liver/testes exposed to hypoxia and/or 4tOP for cellular/physiological changes. Endpoints examined included mRNA expression from real-time PCR of HIF-1α, HIF-2α, PHD3, EPO, and VTG using cDNA from total RNA extracts, and microarray analyses of genes expressed during the transition from hypoxia back to normoxia. Phylogenetic analyses confirmed isolation of two HIF-α isofoms (HIF-1α and HIF-2α) and the PHD3 isoform. Significant up-regulation of PHD3 occurred within 10 hrs of chronic hypoxia, and persisted when severe (1.5 mg/L) and declined when moderate (~2.5mg/L). Significant up-regulation of HIF-1α and EPO occurred within 30 minutes to 2 hours of onset of acute severe and very severe (~1.08mg/L) hypoxia. Hypoxia acted similar to an estrogen mimic, with huge up-regulation of VTG gene expression in males, and increased VTG levels (additive effect) when hypoxia was combined with 4tOP. Microarray analyses showed 125 genes with significant transcriptional change, with up- or down-regulation from transitions of: (1) hypoxia (72 hrs) to normoxia (74 hrs) and (2) hypoxia+4tOP (72 hrs) to normoxia+4tOP (74 hrs).

Movement Ecology Of An Intercontinental Migratory Bird During Spring Stopover, Emily Beth Cohen

Dissertations

Movement ecology is a component of nearly all aspects of animal behavior and an animal’s decision to move is likely influenced by a complex combination of exogenous and endogenous factors. Therefore, an examination of the causes and consequences of organismal movement provides a conceptual framework for understanding complex behavioral strategies. My dissertation research is focused on the movement ecology of an intercontinental migratory songbird during spring migration. I adopted experimental approaches to study the factors influencing how a songbird migrant, red-eyed vireos (Vireo olivaceus), makes decisions in unfamiliar landscapes from the initiation of spring stopover.

I simulated the …

The Relations Among Laterality, Cortisol, And Approach-Avoidance Behavior In Garnett's Bushbaby (Otolemur Garnettii), David Burton Hanbury

Dissertations

Many studies to date have demonstrated that approach and avoidance behaviors are processed asymmetrically in the brain and may be reflected in measures such as handedness. The purpose of this study was to extend work in primates on this topic to Garnett’s bushbaby, a prosimian species. Furthermore, to determine whether measures in addition to handedness relate to approach-avoidance behavior, lateralized differences in tympanic membrane temperature were assessed. Cortisol measures were also obtained to determine whether it was related approach-avoidance behavior and handedness. Eleven captive-born Garnett’s bushbabies (Otolemur garnettii) were evaluated for handedness and responsiveness to novelty. Moreover, the …