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The Effect Of Postruminal Amino Acid Flow On Muscle Cell Proliferation And Protein Turnover, James M. Reecy, James E. Williams, Monty S. Kerley, Ruth S. Macdonald, William H. Thornton, Julie L. Davis
The Effect Of Postruminal Amino Acid Flow On Muscle Cell Proliferation And Protein Turnover, James M. Reecy, James E. Williams, Monty S. Kerley, Ruth S. Macdonald, William H. Thornton, Julie L. Davis
Ruth S. MacDonald
An experiment was conducted to characterize the effects of postruminal administration of casein, glutamine, cornstarch, and water on protein turnover and in vitro muscle cell proliferation. Four MARC III steers (205 kg) were fed a protein-restricted bromegrass hay-based diet (2.86 Mcal of DE/kg and 13.6 g of N/kg). Using a 4 x 5 Latin square arrangement balanced for residual effects, casein and glutamine, equal to 50% of basal dietary nitrogen intake, cornstarch, isocaloric with casein infusion, or an equal volume of water was continuously infused into the abomasum of steers. Blood samples, collected every 2 h for 24 h after …
2,3, 7,8-Tetrachlorodibenzo-P-Dioxin, Steven P. Bradbury
2,3, 7,8-Tetrachlorodibenzo-P-Dioxin, Steven P. Bradbury
Steven P. Bradbury
Since 1985, the United States Environmental Protection Agency (USEPA) has classified 2,3,7,8-tetrachloro-p-dioxin (TCDD) as a probable human carcinogen; subsequently, sources of TCDD in the environment have been regulated on the basis of animal cancer rates extrapolated to doses associated with human exposures. Two major activities have prompted the decision to reassess this approach for evaluating TCDD toxicity and its associated risks. First, an epidemiological study of cancer mortality in U.S. chemical workers by the National Institute of Occupational Safety and Health provided evidence of TCDD-mediated human carcinogenicity (Fingerhut et al. 1991 ). Second, at a 1990 Banbury conference a consensus …
Comparison Of The Effect Of Different Opsonins On The Phagocytosis Of Fluorescein-Labeled Staphylococcal Bacteria By Chicken Heterophils, Claire B. Andreasen, James R. Andreasen Jr., Anita E. Sonn, Julie A. Oughton
Comparison Of The Effect Of Different Opsonins On The Phagocytosis Of Fluorescein-Labeled Staphylococcal Bacteria By Chicken Heterophils, Claire B. Andreasen, James R. Andreasen Jr., Anita E. Sonn, Julie A. Oughton
Claire B. Andreasen
Heterophil phagocytosis of fluorescein-labeled staphylococcal bacteria was analyzed by flow cytometry. Opsonization with two types of normal pooled sera and staphylococcal antisera significantly increased bacterial phagocytosis compared to samples without an opsonin. The staphylococcal antisera did not significantly increase bacterial phagocytosis compared to the normal pooled sera. Opsonization appears to increase bacterial phagocytosis but specific antisera may not increase phagocytosis beyond that caused by pooled normal sera.
Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For Quantification Of Skeletal Muscle Calpastatin, M. E. Doumit, Steven M. Lonergan, J. R. Arbona, J. Killefer, M. Koohmaraie
Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For Quantification Of Skeletal Muscle Calpastatin, M. E. Doumit, Steven M. Lonergan, J. R. Arbona, J. Killefer, M. Koohmaraie
Steven M. Lonergan
An indirect antibody ELISA was developed for rapid and sensitive quantification of skeletal muscle calpastatin. Polyclonal antibodies were raised in rabbits against recombinant calpastatin, corresponding to domains 2, 3, and 4 of bovine skeletal muscle calpastatin. Western blot analysis revealed that these antibodies specifically recognize an immunoreactive calpastatin protein of approximately 130 kDa in prerigor skeletal muscle extracts. The intensity of the immunoreactive bands corresponds qualitatively with assayable calpastatin activity. For ELISA development, optimum dilutions of sample, primary anti-calpastatin antibody, and peroxidase-conjugated secondary antibody were determined by titration. A dilution optimum for coating of Immulonâ 4 (Dynatech) plates was observed …