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Effect Of Rotational Or Continuous Stocking Method Of Winter Pasture On Beef Heifer Performance, David G. Skeans Jan 2009

Effect Of Rotational Or Continuous Stocking Method Of Winter Pasture On Beef Heifer Performance, David G. Skeans

LSU Master's Theses

Considerable research has been conducted comparing the effect of continuous and some form of rotational stocking method on animal and forage performance, but most research utilizes put-and-take stocking rates. The purpose of this research was to determine the effect of 12-paddock rotational stocking method (ROT) or continuous stocking method (CONT) of winter wheat (Triticum aestivum) and annual ryegrass (Lolium multiflorum) pastures on beef heifer and forage performance while maintaining a continuous stocking rate of 3.7 heifers/ha. Two experiments were conducted and analyzed separately due to confounding year and location. Heifer ADG and WG/ha tended to be increased by ROT compared …


Pulsed Electric Field Influences On Acid Tolerance, Bile Tolerance, Protease Activity And Growth Characteristics Of Lactobacillus Acidophilus La-K, Olga Antonina Cueva Jan 2009

Pulsed Electric Field Influences On Acid Tolerance, Bile Tolerance, Protease Activity And Growth Characteristics Of Lactobacillus Acidophilus La-K, Olga Antonina Cueva

LSU Master's Theses

Pulsed electric field processing represents a promising non-thermal technology which involves the application of pulses of high voltage (20-80 kV/cm) for less than one second to fluid foods placed between two electrodes. During the application of certain PEF conditions microbial inactivation occurs by causing a pore formation and destruction of the cell membranes. Lactobacillus acidophilus is an important probiotic bacterium used for the production of fermented dairy products. The objective of this study was to study the influence of pulsed electric field (PEF) on the growth characteristics, bile tolerance, acid tolerance, and protease activity of Lactobacillus acidophilus LA-K. Freshly thawed …


Determining Gene Copy Number In Transfected Caprine Fibroblast Cells, Jessica A. Wilson Jan 2009

Determining Gene Copy Number In Transfected Caprine Fibroblast Cells, Jessica A. Wilson

LSU Master's Theses

Transgene expression in stably transgenic organisms is affected by many factors, including the copy number of the transgene in the genome, and by interactions between the transgene and flanking DNA sequences. Transgene copy number has also been shown to effect genetic stability in transgenic plants. Two commonly used methods for transfecting cells are liposome mediated transfection and electroporation. Little is known about the mean transgene copy number or variability of the copy number with these techniques. Quantitative PCR (Q-PCR) has been shown to be an effective method for determining transgene copy number. The objective of this study was to determine …


Dose Titration Of Sericea Lespedeza Leaf Meal On Haemonchus Contortus Infection In Crossbred Lambs, Dana Alicia Pollard Jan 2009

Dose Titration Of Sericea Lespedeza Leaf Meal On Haemonchus Contortus Infection In Crossbred Lambs, Dana Alicia Pollard

LSU Master's Theses

Gastrointestinal nematode (GIN) parasitism is a problem for small ruminant producers. Haemonchus contortus is the most detrimental because of substantial economic losses. Over the past decades, chemical anthelmintics have been used to control GINs. Recently, GINs have developed resistance to most anthelmintics, and now reliance on anthelmintics is not possible. Alternative control methods are needed. Also, the use of fewer chemicals in agricultural products has called for alternative methods to be less synthetic and more organic. One promising alternative method is the feeding of condensed tannin (CT) containing forages as either fresh forage or dried products such as hay, ground …


The Additive Effect Of Melengestrol Acetate (Mga) Priming And Sodium Monensin On Reproductive Performance In Beef Heifers, Jonathan Lance Roberts Jan 2009

The Additive Effect Of Melengestrol Acetate (Mga) Priming And Sodium Monensin On Reproductive Performance In Beef Heifers, Jonathan Lance Roberts

LSU Master's Theses

Breeding beef heifers to produce calves at 2 years of age could be a profitable management decision. The current practices have proved inefficient, which has justified further research in the area. The objective of this study was to examine the effects of repeated progestogen priming on the attainment of puberty by prepubertal heifers, to evaluate the potential additive effect of progestogen priming plus sodium monensin on the attainment of puberty by prepubertal heifers, to examine subsequent reproductive performance, including the response to synchronization of the estrous cycle, first-service pregnancy rates and overall pregnancy rates and to examine the effects of …


The Hyperinsulinemia-Hyperleptinemia Syndrome In Horses: Assessment Of Methods Of Diagnosis And Differential Effects Of Insulin Injection On Glucose, Glucagon And Nonesterified Fatty Acids In Plasma, Thomas Joseph Caltabilota Jan 2009

The Hyperinsulinemia-Hyperleptinemia Syndrome In Horses: Assessment Of Methods Of Diagnosis And Differential Effects Of Insulin Injection On Glucose, Glucagon And Nonesterified Fatty Acids In Plasma, Thomas Joseph Caltabilota

LSU Master's Theses

Plasma leptin concentrations in horses have been shown to vary widely. However, many factors may perturb these measurements. Previous research has documented a link between hyperinsulinemia and hyperleptinemia in horses. This condition has been speculated to be associated with components of equine metabolic syndrome, such as insulin resistance and laminitis. However, many of the methods accepted in diagnosing these metabolic abnormalities are expensive and often difficult to perform. Thus, the objectives of the experiments in this thesis were 1) to determine if it is possible to differentiate hyperleptinemic horses from normal ones by hormone manipulation with dexamethasone, a glucocorticoid analog; …


Characterization Of The Common Eland (Taurotragus Oryx) Estrous Cycle, Parker M. Pennington Jan 2009

Characterization Of The Common Eland (Taurotragus Oryx) Estrous Cycle, Parker M. Pennington

LSU Master's Theses

Three oryx species and nine of eighteen antelope species across three tribes are considered endangered by the IUCN. Though the common eland (Taurotragus oryx) is not endangered, it lends itself well to the adaption of domestic assisted reproductive techniques due to its large size and calm temperament (Hansen et al., 1985). For these techniques to be used efficiently however, the details of the estrous cycle need to be defined within this and other species. Previously, Nowak (1999) proposed the eland estrous cycle to be ~21 days. In addition to cycle length, specifics of the estrous cycle have yet to be …


Effects Of Warming End Of Lay Broiler Breeder Eggs During The Storage Period On Hatchability, Jennifer Michelle Dowden Jan 2009

Effects Of Warming End Of Lay Broiler Breeder Eggs During The Storage Period On Hatchability, Jennifer Michelle Dowden

LSU Master's Theses

Due to necessary storage of hatching eggs in commercial hatcheries, the embryo is being regressed. End of lay broiler breeder eggs have the poorest hatchability and are most affected by pre incubation storage. Warming these eggs is the only way the embryo can develop. The objective of this study was to study the effects of daily warming of end of lay broiler breeder hatching eggs during the storage period on embryo mortality and hatchability. Six trials were conducted, three trials warmed for three of the four day storage period and three trials warmed for five of the six days storage …


A Mineral Survey Of Louisiana Beef Cow/Calf Production Systems, Kyle Austin Guidry Jan 2009

A Mineral Survey Of Louisiana Beef Cow/Calf Production Systems, Kyle Austin Guidry

LSU Master's Theses

The purpose of this research was to determine the state and regional mineral status of Louisiana forages and beef cattle. Louisiana beef cattle operations (n = 25) were sampled and divided into seven geographical regions, including the northwest (NW), northeast (NE), central (CE), southwest (SW), south central (SC), Florida parishes (FP) and southeast (SE) regions. Over a two year period, water and soil samples were collected from each operation annually, forage samples were collected quarterly in Aug to Sep, Nov to Dec, Feb to Mar and May to June and bovine serum samples were collected twice annually in the fall …


The Isolation And Characterization Of Bovine Adult Derived Adipose Stem Cells For The Use In Nuclear Transfer, Alicia A. Picou Jan 2009

The Isolation And Characterization Of Bovine Adult Derived Adipose Stem Cells For The Use In Nuclear Transfer, Alicia A. Picou

LSU Master's Theses

Since the cloning of Dolly there has been little change in the efficiency of nuclear transfer (NT). Research is beginning to investigate the characteristics of donor cells. Adiposetissue is an abundant source of adult-derived cells that have displayed “stemness” in-vitro(Gimble et al., 2003). The overall goal of this research was to define the in-vitro characteristicsof bovine adipose-derived adult stem cells (ADAS) for the use in NT. Isolation methods weredetermined by a 3 x 3 factorial design. 1 g of subcutaneous fat was collected and subjected to0.10%, 0.25% or 0.50% collagenase type I solution for 1, 2 and 3 h. Nucleated …


Determinants Of Fluid Milk Quality, Jose Luis Vargas Jan 2009

Determinants Of Fluid Milk Quality, Jose Luis Vargas

LSU Master's Theses

The objectives of this study were to provide an overview of fluid milk quality in the U.S., determine if there is a correlation between rapid and traditional tests, determine if results from different analyses correlate with a sensory analysis and to determine any correlation between any of the analyses done in this study. Whole and 2% milks were received from 17 dairy processing plants located in the west, midwest, and southern regions of the U.S in duplicates. Samples were pasteurized at the processing plants by HTST method (161-175oF for 15-25 seconds). For standard plate counts at day 0 no significant …


Effect Of Milk Replacer Composition On Growth And Rumen Development Of Neonatal Holstein Calves, Anthony Joseph Bridges Jan 2009

Effect Of Milk Replacer Composition On Growth And Rumen Development Of Neonatal Holstein Calves, Anthony Joseph Bridges

LSU Master's Theses

A study was conducted to determine effects of differing fat and protein levels in commercial milk replacers on the growth performance and rumen development of neonatal Holstein calves. Fifty-three calves (23 male; 30 female) were assigned to one of three dietary treatments which included 3 milk replacers (20/20; 25/15; 28/20) fed once per day at 10% of birth weight through weaning. Calves were offered a 20% CP calf starter and water free choice beginning on day 5. All calves were weaned on day 42. Body weights were measured at birth and weekly through weaning. Additionally, hip height, wither height, and …