Digital Commons Network^™

An Assessment Of Environmental Dna As A Tool To Detect Fish Species In Headwater Streams, Stephen F. Jane

Masters Theses 1911 - February 2014

Recent years have seen an explosion of interest in the use of freely available DNA present in aquatic systems, otherwise known as environmental DNA (eDNA), as a tool for monitoring aquatic organisms. However, much remains unknown about the behavior of eDNA over a range of environmental conditions. This is particularly true in high gradient headwater streams, which have received less attention than other types of water bodies. In the summer of 2011, a headwater stream system with well established species distributions was sampled using eDNA techniques. Though species were detected where known to be present, detections also occurred where traditional …

Movement Patterns And Catch-And-Release Impacts Of Striped Bass In A Tidal Coastal Embayment In Massachusetts, Heather M. Tyrrell

Masters Theses 1911 - February 2014

An investigation into the spatial ecology and effects of catch-and-release angling on the physiology and behavior of striped bass was conducted. Fine-scale behavior was assessed by tagging fish with acoustic transmitters equipped with pressure and tri-axial accelerometer sensors and tracking them within a fixed array (n=34 receivers) in a Massachusetts estuary. Activity space changed significantly over the course of the season and increased with water temperature. Striped bass most frequently exhibited low levels of locomotory activity representing 67% of total activity measurements, with occasional high activity and burst swimming, often within the upper 3 m of the water column. Depth …

The Effects Of Construction Activity On The Behavior Of Captive Rhesus Monkeys (Macaca Mulatta), Courtney A. Begnoche

Masters Theses 1911 - February 2014

Previous studies have revealed insight into the effects of noises and vibrations on rodents, livestock, and zoo animals, but there is little information about such effects on non-human primates. This study aimed to assess the impact of construction activity on the behavior of animals in a non-human primate (rhesus macaque) facility. Construction activity and modified frequency behavioral data were divided into three phases: baseline (~3 months prior to construction), roof (construction on top of the animal facility), and honors (construction of 7 new buildings adjacent to the facility). We hypothesized that anxiety behaviors (scratch and yawn) would be increased during …

Habitat Heterogeneity Concentrates Predators In The Seascape: Linking Intermediate-Scale Estuarine Habitat To Striped Bass Distribution, Cristina Kennedy

Masters Theses 1911 - February 2014

Predators are key components of aquatic ecosystems and innovative approaches to understanding their spatial distribution are imperative for research, effective management, and conservation. Discontinuities, created by abrupt changes between two unlike entities, are irregularly-distributed, intermediate-scale features that can have a disproportionate effect on organismal distribution within the seascape. Here I use the discontinuity concept to relate the distribution of a predator, striped bass (Morone saxatilis), to physical features within Plum Island Estuary (PIE), MA. I mapped the distribution of 50 acoustically-tagged striped bass during four monthly surveys at 40 sites to evaluate if heterogeneity in physical features concentrated …

Investigating The Endocrine Disrupting Potential Of The Effluent-Dominated Assabet River, Kasie M. Auger

Masters Theses 1911 - February 2014

The Assabet River located in eastern Massachusetts receives treated wastewater

discharges from four major municipal wastewater treatment plants (WWTPs): Westborough, Marlborough, Hudson and Maynard. In periods of low flow, up to 95% of the Assabet River is wastewater effluent. Chemical analyses have shown that municipal wastewaters can contain estrogenic and dioxin-like compounds. Estrogenic compounds such as the natural estrogen 17β-estradiol (E2), the synthetic estrogen 17α-ethinyl estradiol (EE2), and the industrial compound nonylphenol (NP) can induce vitellogenin (VTG) and lead to feminization in male fish. CYP1A1-inducing compounds such as the polycyclic aromatic hydrocarbons (PAHs) and polybrominated diphenyl ethers (PBDEs) can produce …

Implementation Of Aquaponics In Education: An Assessment Of Challenges, Solutions And Success, Emily Rose Hart

Masters Theses 1911 - February 2014

Aquaponics is the combination of aquaculture and hydroponic technology to grow both fish and plants together in a closed-loop system. While aquaponics can play a role in increasing food security, it may also be a potential educational tool because of its interdisciplinary nature and required technological skill set. With aquaponics, students could conduct hands-on activities involving chemistry, physics and biology to solidify their understanding of a range of theories. Beyond standard science, technology, engineering and mathematics (STEM) principles, aquaponics may be related to projects on sustainability, environmental science, agriculture, the food system, health, economics, business and marketing. The interdisciplinary nature …

Characterization Of Genes Required For Preimplantation Embryo Development, Marc P. Maserati Jr

Masters Theses 1911 - February 2014

Preimplantation embryo development in the mouse is a time of rapid cellular morphological and molecular changes leading to embryo implantation for the generation of offspring. The Mager lab studies these events occuring between fertilization and implantation in order to better understand the initial events which set the stage for all future aspects of development. The result of this research impacts many scientific disciplines including in-vitro based means of embryo culture, establishment of epigenetic marks, differentiation and cellular reprogramming and can be used in translational research for the improvement of in-vitro culture techniques and develop novel therapies such as cell replacement …

Investigating The Mechanism Of Nur77-Induced Apoptosis In T Cells, Heather E. Fogarty

Masters Theses 1911 - February 2014

Nur77 is a member of the orphan nuclear receptor family, where it is known to play an important role in apoptosis in both negative selection in T cells and in cancer cell lines. In the development of T cells, it is critical for the immune system to discriminate self from non-self by eliminating auto-reactive cells. It was originally thought that Nur77 initiated apoptosis by activating downstream gene targets. However, it is now clear that Nur77 has its own distinct role outside of the nucleus and the precise mechanisms by which Nur77 induces apoptosis in T cells still needs to be …

Diving Behavior And Identification Of Sex Of Breeding Atlantic Puffins (Fratercula Arctica), And Nest-Site Characteristics Of Alcids On Petit Manan Island, Maine, Sarah M. Spencer

Masters Theses 1911 - February 2014

During 2008 – 2009, we quantified foraging behavior of adult Atlantic puffins (Fratercula arctica) by deploying time-depth recorders (TDRs) on 18 adults and collected morphological measurements from 40 adults nesting on Petit Manan Island, Maine. Dive data were successfully retrieved from 5 birds foraging for 14 days in 2008, and 8 birds foraging for 18 days in 2009. Pooling across all birds, a total of 8,097 dives were recorded, with peaks in activity during 0400-0800 and 1600-2000, and no diving between 2100 and 0400. Mean (± SD) dives/bird/day was 276.4 (± 84.7), with dives grouped into bouts lasting …

Population Dynamics, Chick Diet, And Foraging Behavior Of The Razorbill (Alca Torda) At Matinicus Rock, Maine, Katherine E. Kauffman

Masters Theses 1911 - February 2014

During the summers of 2007-2009, I studied the population growth and reproductive and foraging ecology of the Razorbill (Alca torda) at Matinicus Rock (MR), Maine. This medium-sized marine bird in the family Alcidae (auks) was extirpated from the Gulf of Maine in the late 19^th century by hunting, collecting, and colony disturbance. Following legislation protecting seabirds and their nesting habitats, the Razorbill has recolonized probable former nesting habitat in the Gulf of Maine during the past several decades. Six small colonies comprise the Maine population, which is listed as threatened and forms the southern extension of the …

Prairie Warbler Nest-Site Selection, Nest Survival, And Demographic Response To Management In A Pitch Pine-Scrub Oak Barren, Michael E. Akresh

Masters Theses 1911 - February 2014

As shrubland bird populations decline, there is a critical need to understand the effects of habitat management. I studied a population of color-banded prairie warblers (Setophaga discolor) between 2008-2011 in a shifting mosaic landscape within a Massachusetts inland, pitch pine-scrub oak barren consisting of persistent, newly created, succeeding, and disturbed habitats. I present data showing that the abundance and population structure at this site appears to be a function of colonization of newly created habitat by second-year birds, which are likely excluded from mature early-successional habitat by site-faithful older birds. Breeding season fecundity did not differ significantly between …

Temperature Effects On Growth And Stress Physiology Of Brook Trout: Implications For Climate Change Impacts On An Iconic Cold-Water Fish, Joseph G. Chadwick Jr

Masters Theses 1911 - February 2014

Despite the threat of climate change, the physiological mechanisms by which temperature drives the distribution of species are unclear. Here we used chronic temperature exposures to determine that the upper limit for positive growth in the eastern brook trout (Salvelinus fontinalis) is 23.4 °C. Additionally, brook trout exposed to daily temperature oscillations of 8 °C, around a mean of 21 °C, exhibited growth rates that were 43 and 35% lower by length and weight respectively, than in constant 21 °C controls. Limitations in growth were associated with increases in indicators of the physiological stress response. Individuals exposed to …

Factors Affecting Survival And Cause-Specific Mortality Of Saiga Calves (Saiga Tatarica Mongolica) In Mongolia, Buuveibaatar Bayarbaatar

Masters Theses 1911 - February 2014

Factors affecting juvenile survival are poorly known in the world’s most northern antelope, the endangered saiga (Saiga tatarica), yet they are fundamental for understanding what drives population change. For saiga neonates monitored in Sharga Nature Reserve, western Mongolia, during 2008–2010, male and single calves were heavier than those of female and twins, respectively. However, there was no significant difference in seasonal and annual survival rate between male and female or singletons and twins. Litter size and birth mass varied among years, and there was a negative relationship between these variables. Multiple regression models suggest that summer precipitation in …

Reproductive Maturation And Diel Reproductive Periodicity In Western Gulf Of Maine Haddock, Katie A. Anderson

Masters Theses 1911 - February 2014

A new macroscopic ovarian reproductive maturity index for haddock, Melanogrammus aeglefinus L, was developed to improve field collection of reproductive stage data. The index was tested, validated and revised based on a comparison with a laboratory histological staging method. The comparison of field and histological observations helped to improve the field index and methodologies and provided useful insight into the reproductive biology of Haddock. Although laboratory staging based on histology is inherently more accurate than any macroscopic field staging method, field observations can reveal weaknesses in the laboratory approach due to sampling bias. The revised field index includes three …

In Vivo Investigations Of Polymer Conjugates As Therapeutics, Elizabeth M. Henchey

Masters Theses 1911 - February 2014

Polymer conjugates offer a way to introduce materials into the body that would normally be rejected or cause toxicity. Two polymers are investigated in vivo for uses in chemotherapeutic delivery, protein therapeutics, and DNA transfection. A novel polymer, polyMPC, has the ability to increase doxorubicin loading and its solubility, and is conjugated in a way to release its payload in a low pH environment. Through its conjugation, blood clearance time of doxorubicin is increased, and thus tumor exposure to the drug is increased with a single administration. It can be administered at ten times the concentration of free doxorubicin, and …

Examination Of Sexually Dimorphic Cell Death In The Pubertal Mouse Brain, Amanda Holley

Masters Theses 1911 - February 2014

A period of cell death during a critical period early in life is responsible for causing permanent structural changes to many brain areas, but it is not known whether cell death plays a role in brain organization outside of early postnatal life. Puberty is considered a second sensitive period because the brain is the target organ of gonadal hormones. This study looked at global and regional patterns of cell death during pre-puberty and puberty in the mouse brain. My findings show there is more cell death happening during pre-puberty than during puberty. Cell death does happen during puberty but at …

Building Integrated Aquaculture, Erik A. Woodin

Masters Theses 1911 - February 2014

Exploratory research into a fresh concept, building integrated aquaculture, has found new information on the topic. Motives indicating building integrated aquaculture is important for sustainable development were identified. A review of the literature found relatively little in the way of experiments, aquaculture operations, and case studies which documented or demonstrated a deep understanding of the interactions between building and aquaculture systems. A simple experiment was conducted observing thermal energy and moisture interactions in simulated aquaculture facilities, some with tank covers and insulation. Two different retrofit programs were developed in a case study of building integrated aquaculture for an existing structure …

A Multidisciplinary Approach To Food Safety Evaluation: Hummus Spoilage And Microbial Analysis Of Kitchen Surfaces In Residential Child Care Institutions (Rcci) In Massachusetts, U.S.A., Elsina E. Hagan

Masters Theses 1911 - February 2014

Food borne illnesses continues to be a public health challenge in the United States (U.S.); an estimated 9.4 million incident cases occurred in 2011. In view of this challenge we conducted two food safety studies; 1) related to product formulation (hummus spoilage challenge study) and 2) evaluating the microbial safety of domestic kitchen surfaces in Residential Child Care Institutions (RCCI pilot study).

Hummus is of Mediterranean origin but is currently eaten globally. This challenge study evaluates a variety of industrial hummus formulations (four in total, differing in pH and/or addition of a preservative (natamycin). Two batches were setup: batch 1; …

Evaluation Of Pre-Spawning Movements Of Anadromous Alewives In The Ipswich River Using Radiotelemetry, Holly J. Frank

Masters Theses 1911 - February 2014

Conserving and restoring anadromous fish populations is an important research and management priority. For conservation to be effective, researchers must understand the behavior of the fish they seek to restore. Telemetry has allowed researchers to understand the upstream migrations of these fish in freshwater, how migration patterns vary, and if there is a relationship between behavior and environmental variables. In the northeastern United States, alewife (Alosa pseudoharengus), one of two species collectively referred to as river herring, has historically been an important component of coastal rivers. However, populations of these fish have experienced recent declines, and a commonly used method …

Conservation Implications Of A Marbled Salamander, Ambystoma Opacum, Metapopulation Model, Ethan B. Plunkett

Masters Theses 1911 - February 2014

Amphibians are in decline globally and a significantly greater percentage of ambystomatid salamander species are in decline relative to other species; habitat loss contributes significantly to this decline. The goals of this thesis is to better understand extinction risk in a marbled salamander (ambystoma opacum) population and how forestry effects extinction risk. To achieve this goal we first estimated an important life history parameter (Chapter 1) then used a metapopulation model to estimate population viability and determine what aspects of their life history put them most at risk (Chapter 2) and finally predicted extinction risk in response to hypothetical forestry …

Variation In Winter Estuarine Habitat Use By Bluefish In Northeastern Florida With Implications For Growth And Condition, John S. Murt

Masters Theses 1911 - February 2014

Age and growth were determined from otoliths for 181 juvenile bluefish, Pomatomus saltatrix, collected using a variety of gear in northeast Florida during 2003 and 2005. Three distinct cohorts were identified recruiting to the near shore waters during spring, summer and fall. Growth rates were high regardless of cohort or season. To compare pre- and post-recruitment growth rates, models were fit to individual growth trajectories using change point analysis. Post-estuarine growth rates were generally higher. Growth rates and hatching times were within the range of those obtained in other bluefish studies conducted at higher latitudes. As this is the only …

Spatial And Temporal Dynamics Of Land Use Impacts On Water Quality In Watershed Systems, Olga Tsvetkova

Masters Theses 1911 - February 2014

ABSTRACT SPATIAL AND TEMPORAL DYNAMICS OF LAND USE IMPACTS ON WATER QUALITY IN WATERSHED SYSTEMS SEPTEMBER 2007 OLGA TSVETKOVA, B.S., NOVGOROD STATE UNIVERSITY, RUSSIA M.S., UNIVERSITY OF MASSACHUSETTS AMHERST Directed by: Professor Timothy O. Randhir Predicting land use change and assessing watershed tradeoffs between the watershed system components through system simulation helps to determine future nutrient and sediment load reductions needed to obtain a particular water quality standard. This also helps to examine the tradeoffs among nutrient and sediment load reductions that achieve the same water quality objective. Tradeoff assessment is a useful tool to meet agricultural and urban needs …