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The Effect Of Silencing The Wilms' Tumor 1 Gene On The Radiation Sensitivity Of Glioblastoma Cells, Dana C. Chan
The Effect Of Silencing The Wilms' Tumor 1 Gene On The Radiation Sensitivity Of Glioblastoma Cells, Dana C. Chan
Theses and Dissertations
Glioblastomas are among the most devastating of human cancers with a median survival of only 9-12 months. This type of brain tumor is incurable, largely due its remarkable proliferative capacity and resistance to current treatments. High levels of the Wilms' Tumor 1 (WTI) gene have been identified in glioblastomas, suggesting an oncogenic function. Moreover, known WT1 target genes have been implicated in resistance to radiation. To determine the role of WT1 in radiation resistance, two glioblastoma cell lines expressing WT1 were treated with siRNAs to silence this gene. Confirmation of WT1 knockdown was achieved through real-time PCR and Western blot. …