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Immuno-Histochemical Method For Demonstration Of Lactic Dehydrogenase "M" Subunits In Rat Skeletal Muscle, Kidney And Peripheral Nerve, Luis M. Bustos-Arango
Immuno-Histochemical Method For Demonstration Of Lactic Dehydrogenase "M" Subunits In Rat Skeletal Muscle, Kidney And Peripheral Nerve, Luis M. Bustos-Arango
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Immunolocalization of lactic dehydrogenase (LDH) rrM'‘' sub units has been achieved using paraformaldehyde vapor phase fixation of freeze-dried sections. An antiserum to monkey LDH-M^ was found to cross react with rat LDH-M subunits. The fibers of the gastrocnemius and soleus muscles which are stained using the immuno-peroxidase procedure do not stain in the presence of 4 M urea in the conventional method. In kidney, the collecting tubules are the structures which stain most intensely with the immuno-peroxidase procedure and in peripheral nerve both the Schwann cells and axons stain. The nuclei of muscle fibers. kidney tubular cells and Schwann cells …