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Development Of Pre-Clinical Assays Based On Tandem Mass Spectrometry To Investigate Gabaa Receptor Modulators, Margaret Laurel Guthrie

Theses and Dissertations

ABSTRACT

DEVELOPMENT OF PRE-CLINICAL ASSAYS BASED ON TANDEM MASS SPECTROMETRY TO INVESTIGATE GABAA RECEPTOR MODULATORS

by

Margaret L. Guthrie

The University of Wisconsin – Milwaukee, 2017

Under the Supervision of Professor Alexander (Leggy) Arnold

Drugs are compounds that interact with a biological system to produce a specific biological response. The goal of drug discovery is to design and synthesize a pharmaceutical agent that will produce a desired biological effect. However, the in-vivo effects of a drug molecule cannot be predicted without a variety of test results prior to human clinical trials. In order to designate any drug compound as a …

Chemometric Curve Resolution For Quantitative Liquid Chromatographic Analysis, Daniel W. Cook

Theses and Dissertations

In chemical analyses, it is crucial to distinguish between chemical species. This is often accomplished via chromatographic separations. These separations are often pushed to their limits in terms of the number of analytes that can be sufficiently resolved from one another, particularly when a quantitative analysis of these compounds is needed. Very often, complicated methods or new technology is required to provide adequate separation of samples arising from a variety of fields such as metabolomics, environmental science, food analysis, etc.

An often overlooked means for improving analysis is the use of chemometric data analysis techniques. Particularly, the use of chemometric …