Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Entire DC Network
Comparability Of Raman Spectroscopic Configurations: A Largescale Cross-Laboratory Study, Shuxia Guo, Hugh Byrne, Shiyamala Duraipandian, Fiona Lyng, Claudia Beleites, Ute Neugebauer
Comparability Of Raman Spectroscopic Configurations: A Largescale Cross-Laboratory Study, Shuxia Guo, Hugh Byrne, Shiyamala Duraipandian, Fiona Lyng, Claudia Beleites, Ute Neugebauer
Articles
The variable configuration of Raman spectroscopic platforms is one ofthe major obstacles in establishing Raman spectroscopy as a valuable physicochemicalmethod within real-world scenarios such as clinical diagnostics. For such real worldapplications like diagnostic classification, the models should ideally be usable to predictdata from different setups. Whether it is done by training a rugged model with data frommany setups or by a primary-replica strategy where models are developed on a‘primary’setup and the test data are generated on‘replicate’setups, this is only possible if the Raman spectra from different setups are consistent, reproducible, and comparable.However, Raman spectra can be highly sensitive to the …
A Comparison Of Raman, Ftir And Atr-Ftir Micro Spectroscopy For Imaging Human Skin Tissue Sections., Syed Mehmood Ali, Franck Bonnier, Helen Lambkin, Kathleen Flynn, V Mcdonagh, Claragh Healy, T C. Lee, Fiona Lyng, Hugh Byrne
A Comparison Of Raman, Ftir And Atr-Ftir Micro Spectroscopy For Imaging Human Skin Tissue Sections., Syed Mehmood Ali, Franck Bonnier, Helen Lambkin, Kathleen Flynn, V Mcdonagh, Claragh Healy, T C. Lee, Fiona Lyng, Hugh Byrne
Articles
Raman and Infrared absorption spectroscopies are compared for the analysis of human hand skin tissue sections. The tissue sections have been formalin fixed and paraffin processed, and subsequently dewaxed. Fourier Transform infrared (FTIR) spectra are preprocessed using the resonant Mie – extended multiplicative scattering algorithm to remove spectral artefacts. FTIR images of resolution 4cm-1, analysed using K-means cluster analysis, reveal the double layer structure of the dermis and epidermis, but no further layer differentiation is achieved using the higher spatial resolution of the Attenuated Total Reflection imaging or improved spectral resolution of 2cm-1. At comparable spectral …