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Molecular Genetic Analysis Of Drought Resistance And Productivity Traits Of Rice Genotypes, Yheni Dwiningsih Dec 2020

Molecular Genetic Analysis Of Drought Resistance And Productivity Traits Of Rice Genotypes, Yheni Dwiningsih

Graduate Theses and Dissertations

Rice (Oryza sativa L.) is the staple food for a majority of the world’s population, and uses 30% of the global fresh water during its life cycle. Drought at the reproductive stage is the most important abiotic stress factor limiting grain yield. The United States is the third largest exporter of rice, and Arkansas is the top rice-producing state. The Arkansas rice-growing region in the Lower Mississippi belt is among the 10 areas with the highest risk of water scarcity. Adapted U.S. rice cultivars were screened for drought resistant (DR) traits to find sources for breeding U.S. rice cultivars for …


Genetic Architecture Of Salt And Drought Tolerance In Cowpea, Waltram Second Ravelombola Jul 2020

Genetic Architecture Of Salt And Drought Tolerance In Cowpea, Waltram Second Ravelombola

Graduate Theses and Dissertations

Cowpea [Vigna unguiculata (L.) Walp.] is a diploid and nutrient-dense legume species. It provides affordable source of protein to human. Cowpea cultivation is prevalent in Africa, Asia, the western and southern U.S., and Central and South America. However, earlier reports have shown that drought and salt stress can be devastating to cowpea production. The objectives of this study were to screen for salt and drought tolerance in cowpea and to identify molecular markers associated with these traits. Simple methodologies to screen for drought (Chapter 2) and salt tolerance were developed (Chapter 3). Results suggested that: 1) a total of 14, …


Genetics Of Physiological Traits Associated With Drought Tolerance In Soybean (Glycine Max), Sumandeep Kaur Bazzer Jul 2020

Genetics Of Physiological Traits Associated With Drought Tolerance In Soybean (Glycine Max), Sumandeep Kaur Bazzer

Graduate Theses and Dissertations

Soybean (Glycine max L.) is one of the major row crops in the United States, and its production is often limited by drought stress. Physiological traits from exotic germplasm that confer drought tolerance may be useful in improving commercial soybean production. For example, carbon isotope ratio (δ13C) is positively correlated with water use efficiency (WUE), and nitrogen isotope ratio (δ15N) is negatively correlated with N2 fixation; canopy temperature (CT) is an indicator for genetic variation in transpiration and stomatal conductance. Therefore, the objectives of this research were to identify the genomic regions associated with: (1) δ13C and δ15N using a …


Characterization Of Squamosa Promoter Binding Protein Like (Spl), And Its Role In Drought Stress Tolerance In Medicago Sativa (Alfalfa)., Alexandria K. Hanly Jun 2020

Characterization Of Squamosa Promoter Binding Protein Like (Spl), And Its Role In Drought Stress Tolerance In Medicago Sativa (Alfalfa)., Alexandria K. Hanly

Electronic Thesis and Dissertation Repository

Traditional breeding involving Medicago sativa (alfalfa), has resulted in minimal yield increases. Moreover, extreme environmental conditions threaten to further limit production. Strategies that make use of molecular tools – such as small non-coding RNA, miR156 – represent an innovative means by which to influence tolerance to abiotic stress. miR156 functions, at least in part, through the SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) family of transcription factors. In this study, the role of SPL9 in regulating alfalfa development and drought tolerance is evaluated. Examination of alfalfa plants with RNAi-mediated SPL9 (SPL9-RNAi) showed that plant height, stem thickness, and internode …


The Lateral Root Density Gene Regulates Root Growth During Water Stress In Wheat, Dante F. Placido, Jaspreet Sandhu, Shirley Sato, Natalya Nersesian, Truyen Quach, Thomas Clemente, Paul Staswick, Harkamal Walia Jan 2020

The Lateral Root Density Gene Regulates Root Growth During Water Stress In Wheat, Dante F. Placido, Jaspreet Sandhu, Shirley Sato, Natalya Nersesian, Truyen Quach, Thomas Clemente, Paul Staswick, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Drought stress is the major limiting factor in agriculture. Wheat, which is the most widely grown crop in the world, is predominantly cultivated in drought-prone rainfed environments. Since roots play a critical role in water uptake, root response to water limitations is an important component for enhancing wheat adaptation. In an effort to discover novel genetic sources for improving wheat adaptation, we characterized a wheat translocation line with a chromosomal segment from Agropyron elongatum, a wild relative of wheat, which unlike common wheat maintains root growth under limited-water conditions. By exploring the root transcriptome data, we found that reduced …


The Lateral Root Density Gene Regulates Root Growth During Water Stress In Wheat, Dante F. Placido, Jaspreet Sandhu, Shirley Sato, Natalya Nersesian, Truyen Quach, Thomas E. Clemente, Paul E. Staswick, Harkamal Walia Jan 2020

The Lateral Root Density Gene Regulates Root Growth During Water Stress In Wheat, Dante F. Placido, Jaspreet Sandhu, Shirley Sato, Natalya Nersesian, Truyen Quach, Thomas E. Clemente, Paul E. Staswick, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Drought stress is the major limiting factor in agriculture. Wheat, which is the most widely grown crop in the world, is predominantly cultivated in drought-prone rainfed environments. Since roots play a critical role in water uptake, root response to water limitations is an important component for enhancing wheat adaptation. In an effort to discover novel genetic sources for improving wheat adaptation, we characterized a wheat translocation line with a chromosomal segment from Agropyron elongatum, a wild relative of wheat, which unlike common wheat maintains root growth under limited-water conditions. By exploring the root transcriptome data, we found that reduced …


Leveraging Genome-Enabled Growth Models To Study Shoot Growth Responses To Water Deficit In Rice, Malachy T. Campbell, Alexandre Grondin, Harkamal Walia, Gota Morota Jan 2020

Leveraging Genome-Enabled Growth Models To Study Shoot Growth Responses To Water Deficit In Rice, Malachy T. Campbell, Alexandre Grondin, Harkamal Walia, Gota Morota

Department of Agronomy and Horticulture: Faculty Publications

lucidating genotype-by-environment interactions and partitioning its contribution to phenotypic variation remains a challenge for plant scientists. We propose a framework that utilizes genome-wide markers to model genotype-specific shoot growth trajectories as a function of time and soil water availability. A rice diversity panel was phenotyped daily for 21 d using an automated, high-throughput image-based, phenotyping platform that enabled estimation of daily shoot biomass and soil water content. Using these data, we modeled shoot growth as a function of time and soil water content, and were able to determine the time point where an inflection in the growth trajectory occurred. We …