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Development Of Mping-Based Activation Tags For Crop Insertional Mutagenesis, Alexander Johnson, Edward Mcassey, Stephanie Diaz, Jacob Reagin, Priscilla S. Redd, Daymond R. Parrilla, Hanh T. Nguyen, Adrian Stec, Lauren A.L. Mcdaniel, Thomas E. Clemente, Robert M. Stupar, Wayne A. Parrott, C Nathan Hancock Dec 2020

Development Of Mping-Based Activation Tags For Crop Insertional Mutagenesis, Alexander Johnson, Edward Mcassey, Stephanie Diaz, Jacob Reagin, Priscilla S. Redd, Daymond R. Parrilla, Hanh T. Nguyen, Adrian Stec, Lauren A.L. Mcdaniel, Thomas E. Clemente, Robert M. Stupar, Wayne A. Parrott, C Nathan Hancock

Department of Agronomy and Horticulture: Faculty Publications

Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to induce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis …


Assessing Approaches For Stratifying Producer Fields Based On Biophysical Attributes For Regional Yield-Gap Analysis, Spyridon Mourtzinis, Patricio Grassini, Juan I. Rattalino Edreira, José F. Andrade, Peter M. Kyveryga, Shawn P. Conley May 2020

Assessing Approaches For Stratifying Producer Fields Based On Biophysical Attributes For Regional Yield-Gap Analysis, Spyridon Mourtzinis, Patricio Grassini, Juan I. Rattalino Edreira, José F. Andrade, Peter M. Kyveryga, Shawn P. Conley

Department of Agronomy and Horticulture: Faculty Publications

Large databases containing producer field-level yield and management records can be used to identify causes of yield gaps. A relevant question is how to account for the diverse biophysical background (i.e., climate and soil) across fields and years, which can confound the effect of a given management practice on yield. Here we evaluated two approaches to group producer fields based on biophysical attributes: (i) a technology extrapolation domain spatial framework (‘TEDs’) that delineates regions with similar (long-term average) annual weather and soil water storage capacity and (ii) clusters based on field-specific soil properties and weather during each crop …


Soybean Response To Water: Trait Identification And Prediction, Shawn Jenkins Feb 2020

Soybean Response To Water: Trait Identification And Prediction, Shawn Jenkins

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The rising demand for soybean [Glycine Max (L.) Merrill] taken in consideration with current climatic trends accentuates the importance of improving soybean seed yield response per unit water (WP). To further our understanding of the quantitative WP trait, a multi-omic approach was implemented for improved trait identification and predictive modeling opportunities. Through the evaluation of two recombinant inbred line populations jointly totaling 439 lines subjected to contrasting irrigation treatments, informative agronomic, phenomic, and genomic associations were identified. Across both populations, relationships were identified between lodging at maturity (r = -0.58, H = 0.86), canopy to air temperature differential …


Organic Soybean Variety Trial, Heather Darby, Sara Ziegler, Ivy Krezinski, Rory Malone Jan 2020

Organic Soybean Variety Trial, Heather Darby, Sara Ziegler, Ivy Krezinski, Rory Malone

Northwest Crops & Soils Program

In 2020, the University of Vermont Extension Northwest Crops and Soils Program evaluated yield and quality of short season organic soybean varieties at Borderview Research Farm in Alburgh, VT. Soybeans can be grown for human consumption, animal feed, and biodiesel. As farmers look to reduce feed costs or diversify markets, soybean acreage across Vermont is increasing. Local research is needed to identify varieties that are best adapted to this region. In an effort to support and expand the local soybean market throughout the northeast, the University of Vermont Extension Northwest Crop and Soils (NWCS) Program established a trial in 2020 …


Soybean Cover Crop Trial, Heather Darby, Ivy Krezinski, Sara Ziegler Jan 2020

Soybean Cover Crop Trial, Heather Darby, Ivy Krezinski, Sara Ziegler

Northwest Crops & Soils Program

In 2020, the University of Vermont Extension Northwest Crops and Soils Program investigated the impact of various cover crop mixtures on the subsequent soybean crop’s yield and quality at Borderview Research Farm in Alburgh, VT. Soybeans are grown for human consumption, animal feed, and biodiesel and can be a useful rotational crop in corn silage and grass production systems. As cover cropping expands throughout Vermont, it is important to understand the potential benefits, consequences, and risks associated with growing cover crops in various cropping systems. In an effort to support the local soybean market and to gain a better understanding …


Conventional Soybean Performance Trials Summary, Heather Darby Jan 2020

Conventional Soybean Performance Trials Summary, Heather Darby

Northwest Crops & Soils Program

Maturity range (0 – 2.1) of soybean varieties in Alburgh, VT

Planting Date: 05-21-2020 Harvest Date: 10-15-2020


Impact Of Planting Date And Variety On Soybean Yield, Heather Darby, Sara Ziegler, Ivy Krezinski, Rory Malone Jan 2020

Impact Of Planting Date And Variety On Soybean Yield, Heather Darby, Sara Ziegler, Ivy Krezinski, Rory Malone

Northwest Crops & Soils Program

In 2020, the University of Vermont Extension Northwest Crops and Soils Program investigated the impact of planting date and variety on soybean yield and quality at Borderview Research Farm in Alburgh, VT. Soybeans can be grown for human consumption, animal feed, and biodiesel. Livestock farmers are interested in producing more of their own grains and as a result, soybean acreage in Vermont is increasing. Given the short growing season in Vermont, it is important to understand optimum planting dates to obtain the highest yields. In an effort to support and expand the local soybean market throughout the northeast, the University …


Organic Soybean Performance Trials Summary, Heathe Darby Jan 2020

Organic Soybean Performance Trials Summary, Heathe Darby

Northwest Crops & Soils Program

Maturity range (0.6 – 1.8) of soybean varieties in Alburgh, VT

Planting Date: 05-21-2020 and Harvest Date: 10-14-2020


Influence Of Burner Position On Temperature Distribution In Soybean Flaming, Miloš Miloš Rajkovic, Goran Malidža, Strahinja Stepanovic, Marko Kostic, Kristina Petrovic Jan 2020

Influence Of Burner Position On Temperature Distribution In Soybean Flaming, Miloš Miloš Rajkovic, Goran Malidža, Strahinja Stepanovic, Marko Kostic, Kristina Petrovic

West Central Research and Extension Center, North Platte

The main objective of this study was to identify optimal burner orientation for a newly designed flame cultivator by quantifying the flame temperature distributions of cross, back, and parallel position of burners at different heights of the soybean canopy (distance from the soil surface). Flame temperatures were measured within-row for three burner orientations at seven propane doses (20–100 kg/ha) and eight different canopy heights (0–18 cm above soil surface). Soybean plants in V3 growth stage were flamed with the same doses and burner orientations, and 28 days after treatment (DAT) crop injury (0%–100%), plant height (cm), dry matter (g) and …


Insufficient Nitrogen Supply From Symbiotic Fixation Reduces Seasonal Crop Growth And Nitrogen Mobilization To Seed In Highly Productive Soybean Crops, Nicolas Cafaro La Menza, Juan Pablo Monzon, John L. Lindquist, Timothy J. Arkebauer, Johannes M.H. Knops, Murray Unkovich, James E. Specht, Patricio Grassini Jan 2020

Insufficient Nitrogen Supply From Symbiotic Fixation Reduces Seasonal Crop Growth And Nitrogen Mobilization To Seed In Highly Productive Soybean Crops, Nicolas Cafaro La Menza, Juan Pablo Monzon, John L. Lindquist, Timothy J. Arkebauer, Johannes M.H. Knops, Murray Unkovich, James E. Specht, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen (N) supply can limit the yields of soybean [Glycine max (L.) Merr.] in highly productive environments. To explore the physiological mechanisms underlying this limitation, seasonal changes in N dynamics, aboveground dry matter (ADM) accumula- tion, leaf area index (LAI) and fraction of absorbed radiation (fAPAR) were compared in crops relying only on biological N2 fixation and available soil N (zero-N treatment) versus crops receiving N fertilizer (full-N treatment). Experiments were conducted in seven high-yield environments without water limitation, where crops received optimal management. In the zero-N treatment, biological N2 fixation was not sufficient to meet the N demand of …