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Synergism Between Entomopathogenic Nematodes And Bacillus Thuringiensis Crops: Integrating Biological Control And Resistance Management, Aaron J. Gassmann, S. Patricia Stock, Mark S. Sisterson, Yves Carriere, Bruce E. Tabashnik
Synergism Between Entomopathogenic Nematodes And Bacillus Thuringiensis Crops: Integrating Biological Control And Resistance Management, Aaron J. Gassmann, S. Patricia Stock, Mark S. Sisterson, Yves Carriere, Bruce E. Tabashnik
Aaron J. Gassmann
The past decade has witnessed a continual increase in the use of crops genetically modified to produce insecticidal toxins from the bacterium Bacillus thuringiensis (Bt). This presents the challenge of designing agricultural systems to manage pests and the evolution of resistance to Bt. We tested whether entomopathogenic nematodes might act synergistically with Bt crops by killing pests in non-Bt refuges and by increasing the fitness costs of resistance to Bt. We also tested whether insect mortality and fitness costs were affected by the cotton phytochemical gossypol. The entomopathogenic nematode Steinernema riobrave increased the fitness cost of Bt resistance, indicating that …
A Comparison Of Three Methods For Determining Root-Knot Nematode Infection Of Cotton Roots, Robert G. Mcbride, Robert Mikkelsen
A Comparison Of Three Methods For Determining Root-Knot Nematode Infection Of Cotton Roots, Robert G. Mcbride, Robert Mikkelsen
Robert Mikkelsen
Three methods for determining the extent of infection of cotton roots (Gossypium hirsutum L.) by root-knot nematode, Meloidogyne incognita (Kofoid and White) Chitwood, were compared. Root-knot nematode eggs were added to pots containing either sterilized soil or soil amended with rye, and cotton seedlings were planted. Cotton root systems were then assessed for infection/damage after 14 and 28 days of exposure. At the 14-day sampling, the roots were stained, using the lactophenol acid fuchsin method and the juvenile nematodes counted. At the 28-day samplings, the cotton roots were given a visual rating for nematode-induced root galling (0-100), and the galls …