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Oxyrase And Pyruvate Inclusion In Extenders For Cold Stored Stallion Spermatozoa, Jordan T. Shore
Oxyrase And Pyruvate Inclusion In Extenders For Cold Stored Stallion Spermatozoa, Jordan T. Shore
MSU Graduate Theses
A current method in addressing subfertility in stallions include manipulating the semen extender media by supplementing antioxidants and energy sources. Two experiments (EXP 1/EXP 2) were conducted to assess the effects of pyruvate and Oxyrase® suspended in commercial diluents for preservation of stallion spermatozoa. Assessment of total (TM) and progressive motility (PM), velocity and direction of movement (VAP, VSL, VCL, and elongation) were recorded by CASA. In EXP 1, 3 different ejaculates were collected from each of 4 stallions. Aliquots of each ejaculate were suspended into 4 treatments of INRA96 with or without Oxyrase® supplemented at 2.4 U/ml …
L-Carnitine And Pyruvate Inclusion In Diluents For Cold-Stored Stallion Spermatozoa, Daniel Scott Potter
L-Carnitine And Pyruvate Inclusion In Diluents For Cold-Stored Stallion Spermatozoa, Daniel Scott Potter
MSU Graduate Theses
Spermatozoa survivability is of utmost importance in delivering equine semen for artificial insemination. Semen extender supplementation may allow for increased survivability. L-carnitine (CARN) has been studied for its role in the mobilization of fatty acids. Two experiments (EX1/EX2) assessed the effects of CARN and pyruvate (P) on spermatozoa survival. Total (TM) and progressive motility (PM), velocity and direction of movement (VAP, VSL, VCL, and elongation) were assessed by CASA, membrane status (SYBR 14/PI stains) and acrosome status (FITC-PSA stain) by flow cytometer. In EX1, 4 ejaculates were collected from 2 stallions. Aliquots were diluted in skim-milk glucose extender (SKMG) or …