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Genome Sequencing Of Ovine Isolates Of Mycobacterium Avium Subspecies Paratuberculosis Offers Insights Into Host Association, John Bannantine, Chia-Wei Wu, Chungyi Hsu, Shiguo Zhou, David C. Schwartz, Darrell O. Bayles, Michael L. Paustian, David P. Alt, Srinand Sreevatsan, Vivek Kapur, Adel M. Talaat
Genome Sequencing Of Ovine Isolates Of Mycobacterium Avium Subspecies Paratuberculosis Offers Insights Into Host Association, John Bannantine, Chia-Wei Wu, Chungyi Hsu, Shiguo Zhou, David C. Schwartz, Darrell O. Bayles, Michael L. Paustian, David P. Alt, Srinand Sreevatsan, Vivek Kapur, Adel M. Talaat
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Background: The genome of Mycobacterium avium subspecies paratuberculosis (MAP) is remarkably homogeneous among the genomes of bovine, human and wildlife isolates. However, previous work in our laboratories with the bovine K-10 strain has revealed substantial differences compared to sheep isolates. To systematically characterize all genomic differences that may be associated with the specific hosts, we sequenced the genomes of three U.S. sheep isolates and also obtained an optical map.
Results: Our analysis of one of the isolates, MAP S397, revealed a genome 4.8 Mb in size with 4,700 open reading frames (ORFs). Comparative analysis of the MAP S397 isolate showed …
Immunogenicity And Protective Efficacy Of The Mycobacterium Avium Subsp. Paratuberculosis Attenuated Mutants Against Challenge In A Mouse Model, Jenn-Wei Chen, Syed M. Faisal, Subhash Chandra, Sean P. Mcdonough, Maria A.S. Moreira, Joy Scaria, Chao-Fu Chang, J. P. Bannantine, Bruce Akey, Yung-Fu Chang
Immunogenicity And Protective Efficacy Of The Mycobacterium Avium Subsp. Paratuberculosis Attenuated Mutants Against Challenge In A Mouse Model, Jenn-Wei Chen, Syed M. Faisal, Subhash Chandra, Sean P. Mcdonough, Maria A.S. Moreira, Joy Scaria, Chao-Fu Chang, J. P. Bannantine, Bruce Akey, Yung-Fu Chang
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Johne’s disease (JD), caused by Mycobacterium avium subsp. paratuberculosis (MAP), results in serious economic losses worldwide especially in cattle, sheep and goats. To control the impact of JD on the animal industry, an effective vaccine with minimal adverse effects is urgently required. In order to develop an effective vaccine, we used allelic exchange to construct three mutant MAP strains, leuD, mpt64 and secA2. The mutants were attenuated in a murine model and induced cytokine responses in J774A.1 cell. The leuD mutant was the most obviously attenuated of the three constructed mutant strains. Our preliminary vaccine trial in mice demonstrated different …