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Biological, Serological, And Molecular Characterization Of Citrus Tristeza Virus Isolates From Different Citrus Cultivation Regions Of Turkey, Savaş Korkmaz, Bayram Çevi̇k, Serkan Önder, N. Kemal Koç
Biological, Serological, And Molecular Characterization Of Citrus Tristeza Virus Isolates From Different Citrus Cultivation Regions Of Turkey, Savaş Korkmaz, Bayram Çevi̇k, Serkan Önder, N. Kemal Koç
Turkish Journal of Agriculture and Forestry
Field surveys were carried out in 5 different citrus cultivation regions of Turkey in 2005 and 2006, and 201 samples were collected from different citrus species. Samples were tested for the presence of Citrus tristeza virus (CTV) by double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). While DAS-ELISA showed that 41 trees were infected with CTV, an additional 13 trees were found to be positive based on RT-PCR. When CTV-positive samples were tested with the Western blot method using the monoclonal antibody MCA13, which is specific to severe isolates of CTV, 32 isolates, mostly from satsuma, …