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Nodulation Reaction To Fungal Infections In Larvae Of Leptinotarsa Decemlineata (Say) (Coleoptera: Chrysomelidae) Mediated By Eicosanoids, Hasan Tunaz, Cemi̇l Bengi̇n, Mehmet Kubi̇lay Er

Turkish Journal of Agriculture and Forestry

Injecting larvae of the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), with eicosanoid biosynthesis inhibitor, phenidone (104 µg larva^{-1}), immediately prior to intrahemocoelic injections of the fungus Beauveria bassiana (Bals.) Vuill. (Deuteromycota: Hyphomycetes) (isolate No: HRI-215: 10 µg larva^{-1} from 2 x 106 blastospores ml^{-1}), sharply reduced (about 75 %) the nodulation reaction to the fungal challenges. Separate treatments with specific inhibitors of the major enzymes involved in eicosanoid biosynthesis, including phospholipase A_2 (PLA_2), cyclooxygenase, and lipoxygenase, reduced nodulation reactions to fungal infections. These findings support the view that nodule formation is a complex process involving lipoxygenase and cyclooxygenase …