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Estrogen Concentrations In Dairy And Swine Waste Storage And Treatment Structures In And Around Tennessee, D. Raj Raman, Elizabeth L. Williams, Robert T. Burns, Alice C. Layton, Adam S. Daugherty, Michael D. Mullen

D. Raj Raman

Naturally occurring estrogens in animal wastes may cause negative environmental impacts in some watersheds. However, there is little published data regarding the concentrations of these estrogenic compounds in full-scale animal waste treatment and storage structures, thus making risk assessment difficult. To address this knowledge gap, the research described in this paper explores estrogen concentrations in 19 animal waste storage and treatment structures at dairy and swine production facilities in and around Tennessee.

Samples have been collected from eight dairy and eleven swine facilities, representing a range of waste storage and treatment structures typical of Tennessee, and much of the southeastern …

Degradation Of Estrogens In Dairy Waste Solids: Effects Of Acidification And Temperature, D. Raj Raman, Alice C. Layton, Lara B. Moody, James P. Easter, Gary S. Sayler, Robert T. Burns

D. Raj Raman

Manure–borne estrogens are increasingly recognized as a potential ecological hazard. However, sample–handling protocols for these compounds are not clearly delineated in the literature, nor are comparisons between assays for estrogens. A study was conducted to explore the degradation of estrogen in separated dairy manure waste solids (press cake), using three popular assay types. Estrogens were measured by enzyme–linked immunosorbent assay (ELISA), gas–chromatography mass–spectroscopy (GC–MS) and a recombinant yeast estrogen reporter assay. As measured by GC–MS, background estrone concentrations were approximately 100 ppb, while 17β–estradiol concentrations were one–third of the estrone concentration, and 17β–estradiol concentrations were below the detection limit (10 …