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Detection And Quantification Of Airborne Conidia Of Fusarium Circinatum, The Causal Agent Of Pine Pitch Canker, From Two California Sites Using A Real Time-Pcr Approach Combined With A Simple Spore Trapping Method, Wolfgang Schweigkofler, Kerry O'Donnell, Matteo Garbelotto
Detection And Quantification Of Airborne Conidia Of Fusarium Circinatum, The Causal Agent Of Pine Pitch Canker, From Two California Sites Using A Real Time-Pcr Approach Combined With A Simple Spore Trapping Method, Wolfgang Schweigkofler, Kerry O'Donnell, Matteo Garbelotto
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Pinus radiata (Monterey pine), a tree native to coastal California and Mexico, is widely planted worldwide for timber production. A major threat to Monterey pine plantations is the fungal disease pine pitch canker, caused by Fusarium circinatum (Hypocreales). We present a novel trapping approach using filter paper in combination with a rapid molecular method to detect the presence of inoculum in the air. The assay is also useful for diagnosing the presence of the pathogen on plants. The test is based on the F. circinatum specific primer pair CIRC1A-CIRC4A, which amplifies a 360-bp DNA fragment in the intergenic spacer region …