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Agriculture

Clemson University

Phenolics

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Secondary Metabolism Inducing Treatments During In Vitro Development Of Turmeric (Curcuma Longa L.) Rhizomes, Matthew M. Cousins, Jeffrey W. Adelberg, Feng Chen, James Rieck Jun 2008

Secondary Metabolism Inducing Treatments During In Vitro Development Of Turmeric (Curcuma Longa L.) Rhizomes, Matthew M. Cousins, Jeffrey W. Adelberg, Feng Chen, James Rieck

Publications

Turmeric (Curcuma longa L.) plants that were grown in vitro for 17 or 22 weeks as a fed-batch culture in 2.5 L vessels yielded 39 to 43 g and 62 to 70 g of fresh rhizomes per vessel, respectively (95 % confidence interval). The MS liquid medium was maintained at 6 % sucrose through media addition twice during the experiment. Various methods were employed in attempts to increase secondary metabolism. Antioxidant and total phenolics assays were employed to characterize phytochemical activity. A first experiment exposed four clones to phenylalanine and/or methyl jasmonate (MeJa) from week 12 to 17 in …


Development Of In Vitro Protocols To Enhance Secondary Metabolite Production From Turmeric (Curcuma Longa L.), Matthew Cousins May 2008

Development Of In Vitro Protocols To Enhance Secondary Metabolite Production From Turmeric (Curcuma Longa L.), Matthew Cousins

All Theses

Plant and organ culture can readily be applied to the field of medicinal plant and secondary metabolite production. Many studies that have been conducted attest to the utility of in vitro methods for preservation of genetic diversity, propagation of clonal individuals for use in commercial field planting, and production of metabolites directly from culture. Experiments have also shown that tissue culture is an important tool for enhancement of medicinal compound production utilizing various methods including elicitation. This thesis focuses on production of metabolites using an in vitro system. An overview in the form of a literature review is provided, which …