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United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

2016

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Low-Intensity, Stocker-Based Channel Catfish Culture, Bartholomew W. Green, Carole R. Engle, Rebecca Lochmann, Harold Phillips Mar 2016

Low-Intensity, Stocker-Based Channel Catfish Culture, Bartholomew W. Green, Carole R. Engle, Rebecca Lochmann, Harold Phillips

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Low-intensity production of Channel Catfish Ictalurus punctatus is characterized by low stocking rates, low installed aeration capacity, and no automated dissolved oxygen monitoring. Two studies conducted in nine 0.25-acre ponds quantified production characteristics of stocker Channel Catfish stocked for low-intensity food-fish production in single- or multiple-batch culture. In the multiple-batch study, 0.5-lb stockers (carryover fish) were stocked at 4,500 fish/acre and 0.044-lb fingerlings at 6,000 fish/acre. Stockers were grown to mean individual weights of 1.25, 1.75, or 2.25 lb. In the single-batch study, 0.5-lb stockers were stocked at 3,500, 4,500, and 5,500 fish/acre. All fish were fed a feed containing …

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Hepatic Transcriptomic And Metabolic Responses Of Hybrid Striped Bass (Morone Saxatilis × Morone Chrysops) To Acute And Chronic Hypoxic Insult, Benjamin H. Beck, S. Adam Fuller, Chao Li, Bartholomew W. Green, Honggang Zhao, Steven D. Rawles, Carl D. Webster, Eric Peatman Jan 2016

Hepatic Transcriptomic And Metabolic Responses Of Hybrid Striped Bass (Morone Saxatilis × Morone Chrysops) To Acute And Chronic Hypoxic Insult, Benjamin H. Beck, S. Adam Fuller, Chao Li, Bartholomew W. Green, Honggang Zhao, Steven D. Rawles, Carl D. Webster, Eric Peatman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Striped bass (Morone saxatilis), white bass (Morone chrysops), and their hybrid are an important group of fish prized for recreational angling in the United States, and there and abroad as a high-value farmed fish. Regardless of habitat, it is not uncommon for fish of the genus Morone to encounter and cope with conditions of scarce oxygen availability. Previously, we determined that hybrid striped bass reared under conditions of chronic hypoxia exhibited reduced feed intake, lower lipid and nutrient retention, and poor growth. To better understand the molecular mechanisms governing these phenotypes, in the present study, we …

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Aspergillus And Aflatoxin In Groundnut (Arachis Hypogaea L.) And Groundnut Cake In Eastern Ethiopia, Abdi Mohammed, Alemayehu Chala, Mashilla Dejene, Chemeda Fininsa, David A. Hoisington, Victor S. Sobolev, R. S. Arias Jan 2016

Aspergillus And Aflatoxin In Groundnut (Arachis Hypogaea L.) And Groundnut Cake In Eastern Ethiopia, Abdi Mohammed, Alemayehu Chala, Mashilla Dejene, Chemeda Fininsa, David A. Hoisington, Victor S. Sobolev, R. S. Arias

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This study was conducted to assess major Aspergillus species and aflatoxins associated with groundnut seeds and cake in Eastern Ethiopia and evaluate growers’ management practices. A total of 160 groundnut seed samples from farmers’ stores and 50 groundnut cake samples from cafe and restaurants were collected. Fungal isolation was done from groundnut seed samples. Aspergillus flavus was the dominant species followed by Aspergillus parasiticus. Aflatoxin analyses of groundnut seed samples were performed using ultra performance liquid chromatography; 22.5% and 41.3% of samples were positive, with total aflatoxin concentrations of 786 and 3135 ng g−1 from 2013/2014 and 2014/2015 …

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A Mycobacterium Avium Subsp. Paratuberculosis Predicted Serine Protease Is Associated With Acid Stress And Intraphagosomal Survival, Abirami Kugadas, Elise A. Lamont, John Bannantine, Fernanda Miyagaki Shoyama, Evan Brenner, Harish K. Janagama, Srinand Sreevatsan Jan 2016

A Mycobacterium Avium Subsp. Paratuberculosis Predicted Serine Protease Is Associated With Acid Stress And Intraphagosomal Survival, Abirami Kugadas, Elise A. Lamont, John Bannantine, Fernanda Miyagaki Shoyama, Evan Brenner, Harish K. Janagama, Srinand Sreevatsan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The ability to maintain intra-cellular pH is crucial for bacteria and other microbes to survive in diverse environments, particularly those that undergo fluctuations in pH. Mechanisms of acid resistance remain poorly understood in mycobacteria. Although, studies investigating acid stress in M. tuberculosis are gaining traction, few center on Mycobacterium avium subsp. paratuberculosis (MAP), the etiological agent of chronic enteritis in ruminants. We identified a MAP acid stress response network involved in macrophage infection. The central node of this network was MAP0403, a predicted serine protease that shared an 86% amino acid identity with MarP in M. tuberculosis. Previous studies confirmed …

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Hypoxia Affects Performance Traits And Body Composition Of Juvenile Hybrid Striped Bass (Morone Chrysops X M. Saxatilis), Bartholomew W. Green, Steven D. Rawles, Sidney A. Fuller, Benjamin H. Beck, Matthew E. Mcentire Jan 2016

Hypoxia Affects Performance Traits And Body Composition Of Juvenile Hybrid Striped Bass (Morone Chrysops X M. Saxatilis), Bartholomew W. Green, Steven D. Rawles, Sidney A. Fuller, Benjamin H. Beck, Matthew E. Mcentire

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Performance traits and body composition of juvenile hybrid striped bass (Morone chrysops x M. saxatilis) in response to hypoxia were evaluated in replicate tanks maintained at constant dissolved oxygen concentrations that averaged 23.0 ± 2.3%, 39.7 ± 3.0%, and 105.5 ± 9.5% dissolved oxygen saturation. Fish were fed a commercially formulated feed daily to apparent satiation. Total feed intake and fish growth and yield increased linearly in response to increased dissolved oxygen concentration. Nutrient utilization was reduced significantly only at the greatest level of hypoxia. With the exception of whole-body protein content, whole body compositional indices and nutrient …

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Nlpc/P60 Domain-Containing Proteins Of Mycobacterium Avium Subspecies Paratuberculosis That Differentially Bind And Hydrolyze Peptidoglycan, J. P. Bannantine, Cari K. Lingle, Philip R. Adam, Kasra X. Ramyar, William J. Mcwhorter, William D. Picking, Brian V. Geisbrecht Jan 2016

Nlpc/P60 Domain-Containing Proteins Of Mycobacterium Avium Subspecies Paratuberculosis That Differentially Bind And Hydrolyze Peptidoglycan, J. P. Bannantine, Cari K. Lingle, Philip R. Adam, Kasra X. Ramyar, William J. Mcwhorter, William D. Picking, Brian V. Geisbrecht

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A subset of proteins containing NlpC/P60 domains are bacterial peptidoglycan hydrolases that cleave noncanonical peptide linkages and contribute to cell wall remodeling as well as cell separation during late stages of division. Some of these proteins have been shown to cleave peptidoglycan in Mycobacterium tuberculosis and play a role in Mycobacterium marinum virulence of zebra fish; however, there are still significant knowledge gaps concerning the molecular function of these proteins in Mycobacterium avium subspecies paratuberculosis (MAP). The MAP genome sequence encodes five NlpC/P60 domain-containing proteins. We describe atomic resolution crystal structures of two such MAP proteins, MAP_1272c and MAP_1204. These …

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Effect Of Interpersonal And Cognitive Stressors On Habituation And The Utility Of Heart Rate Variability To Measure Habituation, Denise M. Feda, James N. Roemmich Jan 2016

Effect Of Interpersonal And Cognitive Stressors On Habituation And The Utility Of Heart Rate Variability To Measure Habituation, Denise M. Feda, James N. Roemmich

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Habituation is a decrease in responding to a repeated stimulus. Operant responding and salivation measure habituation in eating behaviour research. Stress may increase eating by acting as a distractor, yielding spontaneous recovery and prolonging responding for food. Our research tested differences in the ability of cognitive and inter- personal stressors to recover responding for food. We also tested heart rate variability (HRV) as a measure of habituation. Twenty women worked for portions of macaroni and cheese for 15 trials on three separate laboratory visits. Between the 12th and 13th trial, one of three different stressor types (speech, stroop and subtraction) …

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Composition And Potency Characterization Of Mycobacterium Avium Subsp. Paratuberculosis Purified Protein Derivatives, Randal T. Capsel, Charles O. Thoen, Timothy Reinhardt, John D. Lippolis, Renee Olsen, Judith R. Stabel, John P. Bannantine Jan 2016

Composition And Potency Characterization Of Mycobacterium Avium Subsp. Paratuberculosis Purified Protein Derivatives, Randal T. Capsel, Charles O. Thoen, Timothy Reinhardt, John D. Lippolis, Renee Olsen, Judith R. Stabel, John P. Bannantine

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Mycobacterium avium subsp. paratuberculosis (MAP) purified protein derivatives (PPDs) are immunologic reagents prepared from cultured filtrates of the type strain. Traditional pro-duction consists of floating culture incubation at 37°C, organism inactivation by autoclaving, coarse filtration, and protein precipitation. Three traditional production PPDs were used in this study including lot 9801, which served as a reference and has been used in the field for decades. Alternative production PPDs (0902A and 0902B), in which the autoclaving step was removed, were also analyzed in this study. SDS-PAGE analysis revealed protein smearing in traditional PPDs, but distinct bands were observed in the alternative PPD …

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Transcriptional Profiling Of Ileocecal Valve Of Holstein Dairy Cows Infected With Mycobacterium Avium Subsp. Paratuberculosis, Randy J. Hempel, J. P. Bannantine, Judith R. Stabel Jan 2016

Transcriptional Profiling Of Ileocecal Valve Of Holstein Dairy Cows Infected With Mycobacterium Avium Subsp. Paratuberculosis, Randy J. Hempel, J. P. Bannantine, Judith R. Stabel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Johne’s disease is a chronic infection of the small intestine caused by Mycobacterium avium subspecies paratuberculosis (MAP), an intracellular bacterium. The events of pathogen survival within the host cell(s), chronic inflammation and the progression from asymptomatic subclinical stage to an advanced clinical stage of infection, are poorly understood. This study examines gene expression in the ileocecal valve (ICV) of Holstein dairy cows at different stages of MAP infection. The ICV is known to be a primary site of MAP colonization and pro-vides an ideal location to identify genes that are relevant to the progression of this disease. RNA was prepared …

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Phenotype And Function Of Cd209+ Bovine Blood Dendritic Cells, Monocyte-Derived- Dendritic Cells And Monocyte-Derived Macrophages, Kun Taek Park, Mahmoud M. Elnaggar, Gaber S. Abdellrazeq, J. P. Bannantine, Victoria Mack, Lindsay M. Fry, William C. Davis Jan 2016

Phenotype And Function Of Cd209+ Bovine Blood Dendritic Cells, Monocyte-Derived- Dendritic Cells And Monocyte-Derived Macrophages, Kun Taek Park, Mahmoud M. Elnaggar, Gaber S. Abdellrazeq, J. P. Bannantine, Victoria Mack, Lindsay M. Fry, William C. Davis

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Phylogenic comparisons of the mononuclear phagocyte system (MPS) of humans and mice demonstrate phenotypic divergence of dendritic cell (DC) subsets that play similar roles in innate and adaptive immunity. Although differing in phenotype, DC can be classified into four groups according to ontogeny and function: conventional DC (cDC1 and cDC2), plasmacytoid DC (pDC), and monocyte derived DC (MoDC). DC of Artiodactyla (pigs and ruminants) can also be sub-classified using this system, allowing direct functional and phenotypic comparison of MoDC and other DC subsets trafficking in blood (bDC). Because of the high volume of blood collections required to study DC, cattle …

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