Fluid Delivery System For A Cell Culture On A Microfluidic Chip, 2019 California Polytechnic State University, San Luis Obispo
Fluid Delivery System For A Cell Culture On A Microfluidic Chip, Austin J. Roeder, Colleen A. Richards, Emily A. Matteson
This project report provides a description of the progress made in the development of a fluid delivery system for a microfluidic cell culture on a chip. The system is intended to be used in a humidified incubator in a university laboratory and the fluid delivery system is required to exist and operate within that incubator for extended periods of time. Therefore, the system will be gravity-driven and contain no electronic components. The key specification of the system is to provide fluid flow at a constant velocity.
After manufacturing and testing the device, all specifications were met except for the fluid ...
Automated Microscope Stage, 2019 California Polytechnic State University, San Luis Obispo
Automated Microscope Stage, Corin Nishimoto, Alison Flesch, Theo Anastos
This document seeks to describe the background information, customer requirements, design specifications, indications for use, selected materials, proposed budget, prototypes, final design, manufacturing processes, and testing methods regarding the CellOptimizer automated microscope stage product.
Concussion Indication Device, 2019 California Polytechnic State University, San Luis Obispo
Concussion Indication Device, Taylor O'Donoghue, Isabel Jellen, Eric Shechter
In this document we summarize our intent to design and manufacture a device that will be used in conjunction with a football helmet, will detect impact forces to the head, and notify the user to seek out further medical attention when subjected to forces large enough to cause concussions. We describe the large market including all levels of football players and the need for improved technology that will not only be effective in elucidating risks but encouraging players to use the device over the alternatives. To design the device, we have researched the problem of concussions in football and have ...
Plga Scaffold Device, 2019 California Polytechnic State University, San Luis Obispo
Plga Scaffold Device, Tyler D. Tackabery, Bryce Powada, Tate Morell
No abstract provided.
Parkinson's Freezing Of Gait Device Attachment, 2019 California Polytechnic State University, San Luis Obispo
Parkinson's Freezing Of Gait Device Attachment, Reagan Burns, Sidney Collin, Hadley Rose
The Freezing of Gait Project aims to design and develop an attachment mechanism for a medical device to help Parkinson’s patients overcome freezing of gait (FOG). The mechanism will allow the device to attach to a variety of canes or walkers, while being easy for Parkinson’s patients to use. It will interface with a device produced by De Oro Devices. The project includes an analysis of current competitive devices, a summary of customer needs, design of experiments to test significant features of the attachment, and project plan. The customer needs are translated to engineering metrics. Failures and mitigation ...
Heat Stress Identification, 2019 California Polytechnic State University, San Luis Obispo
Heat Stress Identification, Nathanial Nyberg, Christina Santini, Zachary Miller
This document contains all information pertinent to the creation of the desired product and how the desired product fulfills the problem statement. This document clearly defines the customer requirements and how they relate to engineering specifications, metrics that will define the boundaries that the desired product must lie within. This document will then reveal all concepts that were considered during the design phase of this project and how those concepts were compared. This includes all quantitative parameters that provided a clear front-runner concept and the rationale for those parameters and the values and weight assigned to them.
Then this document ...
Flash-Mold Machine For Manufacturing Drug Implants, 2019 California Polytechnic State University, San Luis Obispo
Flash-Mold Machine For Manufacturing Drug Implants, Conor Vache, Sonja Daebelliehn, Ryan Thomson
This Final Project Report provides the reader with a comprehensive detailing of all of the actions and work that has been completed for this Senior Design Project. In this report, the Cal Poly Biomedical Engineering Senior Project Team will discuss some background on the existing Mark I Flash-Mold Machine currently in use by Gesea Biosciences, background information on Gesea Biosciences, the Customer Requirements and Design Specifications of the new machine, Quality Engineering considerations, design concepts discussed, design concepts chosen, safety considerations, prototype development, manufacturing, testing protocols, and testing results, etc.
Hands-Free Ultrasound Transducer Attachment, 2019 California Polytechnic State University, San Luis Obispo
Hands-Free Ultrasound Transducer Attachment, Christina Harrison, Glenn Farina, Harshveer Dhaliwal
This document describes a background on current ultrasound technologies and the troubles that have inspired the need for a hands-free ultrasound transducer device, as well as the regulatory requirements, like patents and standard codes, for the intended invention. It also explains the Stage Gate Review process used to project plan the design of the device, a description of the final design and how it was developed, and the IQ/OQ/PQ. The objectives necessary to design this device, in order to meet all the requirements of the sponsor, are also summarized here. The design process including manufacturing instructions, prototype evolution ...
Esophageal Mapping And Temperature Regulation For Catheter Ablation, 2019 California Polytechnic State University, San Luis Obispo
Esophageal Mapping And Temperature Regulation For Catheter Ablation, Madison J. Keith, Morgan A. Jawitz, Sarah L. Sanders
The purpose of this document is to provide a development summary for a proposed esophageal temperature mapping device used during catheter ablation. Catheter ablation therapy is performed in the left atria with hot or cold materials to create scar tissue to treat atrial fibrillation. However, utilized temperatures and absorbed energies from the catheter can cause undesired esophageal damage. Therefore, this medical device is designed to monitor esophageal temperature and map its location during catheter ablation. If dangerous temperatures are reached, the device will alarm the surgeon.
This document provides an in-depth overview of the esophageal temperaturemapping device’s development and ...
Foot Support Taping Device For Off-Loading Wounds, 2019 California Polytechnic State University, San Luis Obispo
Foot Support Taping Device For Off-Loading Wounds, Andres Fojaco Gomez, Mikaela Williams, Josh Pelegrin
No abstract provided.
Accelerated Wear Reactor Testing, 2019 California Polytechnic State University, San Luis Obispo
Accelerated Wear Reactor Testing, Tristan Frisella, Daniel Alanis, Kian Ashoubi
Carbon dioxide accounts for a large amount of global greenhouse gases released into the environment. Because plastic degradation emits carbon dioxide, there has been interest to investigate how much carbon dioxide various plastics generate as they degrade. We have been tasked to create a dehumidifier and carbon dioxide sensor assembly for an accelerated wear testing apparatus that degrades plastic. Several products and literature currently exist that share similarity with our assembly but have not been manufactured to achieve the same combination of measurements we are expecting of our accelerated wear testing device. The following report shall introduce these products and ...
Placenta‐On‐A‐Chip: In Vitro Study Of Caffeine Transport Across Placental Barrier Using Liquid Chromatography Mass Spectrometry, Rajeendra L. Pemathilaka, Jeremy D. Caplin, Saurabh S. Aykar, Reza Montazami, Nicole N. Hashemi
Mechanical Engineering Publications
Due to the particular structure and functionality of the placenta, most current human placenta drug testing methods are limited to animal models, conventional cell testing, and cohort/controlled testing. Previous studies have produced inconsistent results due to physiological differences between humans and animals and limited availability of human and/or animal models for controlled testing. To overcome these challenges, a placenta‐on‐a‐chip system is developed for studying the exchange of substances to and from the placenta. Caffeine transport across the placental barrier is studied because caffeine is a xenobiotic widely consumed on a daily basis. Since a fetus ...
Cuffed Pediatric Endotracheal Tubes, 2019 Union College
Cuffed Pediatric Endotracheal Tubes, Jennifer Nutter, Emmanuela Oppong, Colleen Pouliot
Endotracheal tubes (ET) are used to provide patients with an adequate airway when they are unable to breathe on their own. ET tubes can be used in emergency situations, surgical procedures, and on-floor intubations. The two main types of ET tubes are cuffed and uncuffed. Due to the fragile structure of a child's throat, uncuffed tubes are primarily used in pediatric patients. Uncuffed tubes can slip out of place resulting in air leakage and improper oxygenation. In contrast, cuffed ET tubes provide a proper seal to the airway and prevent against aspiration. The following work proposes an improved design ...
Direct Observation Of Early Stages Of Growth Of Multilayered Dna-Templated Au-Pd-Au Core-Shell Nanoparticles In Liquid Phase, Nabraj Bhattarai, Tanya Prozorov
Ames Laboratory Accepted Manuscripts
We report here on direct observation of early stages of formation of multilayered bimetallic Au-Pd core-shell nanocubes and Au-Pd-Au core-shell nanostars in liquid phase using low-dose in situ scanning transmission electron microscopy (S/TEM) with the continuous flow fluid cell. The reduction of Pd and formation of Au-Pd core-shell is achieved through the flow of the reducing agent. Initial rapid growth of Pd on Au along <111> direction is followed by a slower rearrangement of Pd shell. We propose the mechanism for the DNA-directed shape transformation of Au-Pd core-shell nanocubes to adopt a nanostar-like morphology in the presence of T30 DNA ...111>
Development Of An Astrocyte/Glioma Co-Culture System For Measuring Cellular Dynamics, 2019 Louisiana Tech University
Development Of An Astrocyte/Glioma Co-Culture System For Measuring Cellular Dynamics, Urna Kansakar
Gliomas are brain tumors that primarily arise from glial cells. Gliomas account for 70% of the brain tumors and they are more prevalent in older adults. About 60% of the people with gliomas experience at least one seizure. Brain tumors can grow and metastasize to neighboring areas, thereby destroying normal brain cells. In a brain tumor microenvironment, both malignant cancer cells and healthy brain cells are present. Studies have shown that astrocytes may have a role in tumor growth in the brain. Monocultures cannot evaluate interactions between two cell types and does not accurately represent in vivo conditions. Thus, a ...
Development Of A Counter-Flow Thermal Gradient Microfluidic Device, 2019 Louisiana Tech University
Development Of A Counter-Flow Thermal Gradient Microfluidic Device, Shayan Davani
This work presents a novel counter-flow design for thermal stabilization of microfluidic thermal reactors. In these reactors, precise control of temperature of the liquid sample is achieved by moving the liquid sample through the thermal zones established ideally through the conduction in the solid material of the device. The goal here is to establish a linear thermal distribution when there is no flow and to minimize the temperature change at flow condition. External convection as well as internal flowinduced effects influence the prescribed thermal distribution. The counter-flow thermal gradient device developed in this study is capable of both stabilizing the ...
Long-Term, Super-Resolution Imaging Of Amyloid Structures Using Transient Amyloid Binding Microscopy, 2019 Washington University in St. Louis
Long-Term, Super-Resolution Imaging Of Amyloid Structures Using Transient Amyloid Binding Microscopy, Tianben Ding, Kevin Spehar, Jan Bieschke, Matthew D. Lew
Electrical & Systems Engineering Publications and Presentations
Amyloid fibrils and tangles are signatures of Alzheimer disease, but nanometer-sized aggregation intermediates are hypothesized to be the structures most toxic to neurons. The structures of these oligomers are too small to be resolved by conventional light microscopy. We have developed a simple and versatile method, called transient amyloid binding (TAB), to image amyloid structures with nanoscale resolution using amyloidophilic dyes, such as Thioflavin T, without the need for covalent labeling or immunostaining of the amyloid protein. Transient binding of ThT molecules to amyloid structures over time generates photon bursts that are used to localize single fluorophores with nanometer precision ...
Muscle Atrophy Marker Expression Differs Between Rotary Cell Culture System And Animal Studies, 2019 Utah State University
Muscle Atrophy Marker Expression Differs Between Rotary Cell Culture System And Animal Studies, Charles P. Harding, Elizabeth Vargis
Biological Engineering Faculty Publications
Muscular atrophy, defined as the loss of muscle tissue, is a serious issue for immobilized patients on Earth and for humans during spaceflight, where microgravity prevents normal muscle loading. In vitro modeling is an important step in understanding atrophy mechanisms and testing countermeasures before animal trials. The most ideal environment for modeling must be empirically determined to best mimic known responses in vivo. To simulate microgravity conditions, murine C2C12 myoblasts were cultured in a rotary cell culture system (RCCS). Alginate encapsulation was compared against polystyrene microcarrier beads as a substrate for culturing these adherent muscle cells. Changes after culture under ...
Simulations Of Hydrogel-Coated Neural Microelectrodes To Assess Biocompatibility Improvement Using Strain As A Metric For Micromotion, Sarah A. Bentil, Rebecca B. Dupaix
Sarah A. Bentil
This study investigates the benefit of coating silicon-substrate microelectrode arrays with hydrogel material for improved biocompatibility. Varying coating thicknesses and hydrogel material descriptions were considered to determine the impact on reducing strain in the surrounding brain tissue caused by relative micromotion of the electrode. Finite element simulations were used to explore biocompatibility by focusing on the longitudinal micromotion of an implanted single electrode shank. The finite element model for the brain and electrode, both with and without the hydrogel coating, remained constant. Three constitutive models were considered to describe the brain and/or hydrogel material: linear elastic, hyperviscoelastic, and fractional ...
Development Of In Situ Forming Hydrogels For Intra-Articular Drug Delivery, 2019 The University of Western Ontario
Development Of In Situ Forming Hydrogels For Intra-Articular Drug Delivery, Andy Prince
Electronic Thesis and Dissertation Repository
Hydrogels are 3-dimensional crosslinked polymer networks that can absorb significant amounts of water. The physical properties associated with hydrogels affords them resemblance to biological tissues making them good candidates for biomedical applications. Many pharmaceuticals, specifically non-steroidal anti-inflammatory drugs (NSAIDs), have poor aqueous solubility, which limits their bioavailability and efficacy. People suffering from chronic osteoarthritis (OA) are required to frequently take large doses to mitigate pain, which can lead to serious side effects. Hydrogels are good strategies to deliver NSAIDs via articular injection because they can form solid gels in situ. This thesis describes the synthesis, formulation, mechanical testing, in vitro ...