The Characterization Of Biofilm Attachment To Metal Interfaces: Effects Of Substratum Properties, 2012 California Polytechnic State University - San Luis Obispo
The Characterization Of Biofilm Attachment To Metal Interfaces: Effects Of Substratum Properties, Marcel D. Mendes
Master's Theses and Project Reports
Bacteria are among the most abundant microorganisms on earth, and can be found in essentially every environment. When a clean surface is exposed to media containing planktonic bacteria, the bacterial cells will attach to the surface and aggregate to form what is known as a biofilm. Biofilms have been shown to negatively affect many industries including medical, industrial, and food science applications. While biofilms have been well characterized from a microbiology perspective, there has been much less research from a materials science standpoint. It is hypothesized that the material properties of the substratum (such as the micro-structure) have a significant ...
A Kinetic Study Of Anti-Vegf-A Polyclonal Antibodies And Anti-Vegf-A Ssdna Aptamers, 2012 California Polytechnic State University, San Luis Obispo
A Kinetic Study Of Anti-Vegf-A Polyclonal Antibodies And Anti-Vegf-A Ssdna Aptamers, Heather A. Hedeen
Master's Theses and Project Reports
A new detection reagent that could possibly augment or replace antibodies research and diagnosis methods are aptamers. Aptamers are ssDNA, RNA or polypeptide constructs that function like active antibodies. Antibodies and aptamers both specifically bind to selected target molecules, and as such they enable the detection or targeting of the presence or absence of a specific antigen.
In order to ensure that ssDNA aptamers perform similarly to antibodies, anti-VEGF-A polyclonal antibody and anti-VEGF-A ssDNA aptamer were evaluated against vascular endothelial growth factor A (VEGF-A) using Surface Plasmon Resonance (SPR). It was hypothesized that the anti-VEGF-A aptamer had the same, if ...
Nitrocellulose Paper Based Microfluidic Platform Development And Surface Functionalization With Anti-Ige Aptamers, 2012 California Polytechnic State University - San Luis Obispo
Nitrocellulose Paper Based Microfluidic Platform Development And Surface Functionalization With Anti-Ige Aptamers, Jennifer Guerin Ward
Master's Theses and Project Reports
The purpose of this thesis project was to demonstrate the ability to utilize the capabilities of aptamers so that they may act as capture reagents for paper microfluidic devices. Several characterization experiments were conducted as a precursor before the final experimentation was performed. Paper characterization, manufacturing protocols for printing and heating, as well as 3D chip fabrication were all performed and analyzed. The results confirmed that the control of fluid through a 3D microfluidic device based in nitrocellulose is possible.
For the biochemistry portion of this thesis report, antibodies and aptamers were chosen to react with IgE, an antibody that ...
Development And Application Of A Portable System To Reliably Measure Grip Forces Using Thin-Film Force Sensors, 2012 University of Connecticut - Storrs
Development And Application Of A Portable System To Reliably Measure Grip Forces Using Thin-Film Force Sensors, Shane Vincent Tornifoglio
Accurate tracking of hand grip force is an important consideration needed for a robust understanding in the study of human biomechanics. One aspect where it proves useful is in hand-arm vibration, such as from gripping a power tool. Depending on how firmly the user is gripping the tool, they may change their exposure levels to the tool vibration, which can lead to potential disorders such as Hand-Arm Vibration Syndrome (HAVS).
A small, battery powered portable force unit has been developed to supply power to, and condition, the signals from eight thin-film force sensors. This gives a better understanding of the ...
The Presence Of Regional Accents In Electrolarynx Speech And The Resultant Effect On Overall Intelligibility., 2012 Dublin Institute of Technology
The Presence Of Regional Accents In Electrolarynx Speech And The Resultant Effect On Overall Intelligibility., Brian Madden, Eugene Coyle
During voiced speech, the larynx provides quasi-periodic acoustic excitation of the vocal tract. In most electrolarynxes, mechanical vibrations are produced by a linear electromechanical actuator, the armature of which percusses against a metal or plastic plate at a frequency within the range of glottal excitation. In this paper, a phonological analysis of a section of results from an online perceptual intelligibility test was performed which compared speech produced using a novel hands-free electrolarynx and a commercially available electrolarynx. A portion of the test consisted of a closed-set format containing a selection of four sets of four random CVC audio samples ...
Evaluation Of Decellularization Procedures For Porcine Arteries, 2012 California Polytechnic State University - San Luis Obispo
Evaluation Of Decellularization Procedures For Porcine Arteries, Charles Clapp
Biomedical Eng/General Eng.
Coronary artery disease has become the leading cause of death in the United States, with over 425,000 deaths in 2006. Stenting has evolved into the preferred preventative technique for myocardial infarction by opening up an occluded artery, due to its low invasiveness compared to the alternative of coronary artery bypass grafting. Bare metal stents have been improved by coating with anti-proliferative drugs to advance their effects, but even drug eluting stents still have a risk of restenosis, thrombus formation, and necessary revascularization. Continual advancement in stent design necessitates faster, effective pre-clinical evaluation techniques. Kristen Cardinal, Ph.D., developed the ...
Living In An Age Of Technology: College Students' Perception Of The Role Of Genetic Engineering And Reproductive Technology In Today's Society, Kayla Quadros
Honors Projects Overview
This study examines attitudes about prevalent issues in genetics and reproduction among the college population. Eighteen interviews were conducted with students at Rhode Island College. Respondents were asked questions about their moral standpoints concerning utilizing genetic engineering for disease control and aesthetic purposes, employing assisted reproductive technologies (ARTs), regulation of such procedures, and also about media sources for which they have heard of these topics. Findings suggest that, generally, participants felt that genetic engineering for health issues is permissible, yet reprehensible for the purpose of aesthetically "designing" a baby, though four students (three of which were female) found the enhancement ...
The Influence Of Heterogeneous Meninges On The Brain Mechanics Under Primary Blast Loading, 2012 University of Nebraska-Lincoln
The Influence Of Heterogeneous Meninges On The Brain Mechanics Under Primary Blast Loading, Linxia Gu, Mehdi S. Chafi, Shailesh Ganpule, Namas Chandra
Mechanical & Materials Engineering Faculty Publications
In the modeling of brain mechanics subjected to primary blast waves, there is currently no consensus on how many biological components to be used in the brain–meninges–skull complex, and what type of constitutive models to be adopted. The objective of this study is to determine the role of layered meninges in damping the dynamic response of the brain under primary blast loadings. A composite structures composed of eight solid relevant layers (including the pia, cerebrospinal fluid (CSF), dura maters) with different mechanical properties are constructed to mimic the heterogeneous human head. A hyper-viscoelastic material model is developed to ...
A Thousand Tiny Pieces: The Federal Circuit’S Fractured Myriad Ruling, Lessons To Be Learned, And The Way Forward, 2012 American University Washington College of Law, Washington D.C.
A Thousand Tiny Pieces: The Federal Circuit’S Fractured Myriad Ruling, Lessons To Be Learned, And The Way Forward, Jonathan R. K. Stroud
No abstract provided.
Assessing The Therapeutic Effect Of 630 Nm Light-Emitting Diodes Irradiation On The Recovery Of Exercise-Induced Hand Muscle Fatigue With Surface Electromyogram, Dandan Yang, Xiaoying Wu, Wensheng Hou, Xiaolin Zheng, Jun Zheng, Yingtao Jiang
Electrical and Computer Engineering Faculty Publications
This paper aims to investigate the effect of light emitting diode therapy (LEDT) on exercise-induced hand muscle fatigue by measuring the surface electromyography (sEMG) of flexor digitorum superficialis. Ten healthy volunteers were randomly placed in the equal sized LEDT group and control group. All subjects performed a sustained fatiguing isometric contraction with the combination of four fingertips except thumb at 30% of maximal voluntary contraction (MVC) until exhaustion. The active LEDT or an identical passive rest therapy was then applied to flexor digitorum superficialis. Each subject was required to perform a re-fatigue task immediately after therapy which was the same ...
Effect Of Size Reduction Parameters In Pharmaceutical Manufacturing Process, 2011 California Polytechnic State University - San Luis Obispo
Effect Of Size Reduction Parameters In Pharmaceutical Manufacturing Process, Brian Breneman
Biomedical Eng/General Eng.
Within pharmaceutical manufacturing, size reduction is one of the most extensively used and vital unit operations. Size reduction in tablet production is achieved primarily through milling procedures, which allows for attaining product uniformity, optimizing product solubility, and improving bioavailability. Additionally, powders with a narrow range of size distribution can obviate problems in downstream processing as pertains to blending, compression, and coating as well as improve drug performance1. The purpose this study was to characterize the effect of various size reduction parameters as a result of the pharmaceutical milling process on a lactose wet granulation formulation and chemically active extruded ...
Impact Of Collateral Enlargement On Smooth Muscle Phenotype, 2011 California Polytechnic State University - San Luis Obispo
Impact Of Collateral Enlargement On Smooth Muscle Phenotype, Alexander Jerome Bynum
Master's Theses and Project Reports
Peripheral Artery Disease is a very serious disease characterized by an arterial occlusion due to atherosclerotic plaques. In response to an arterial occlusion, arteriogenesis occurs, causing smooth muscle cells to transition from a contractile to synthetic state. Also following an arterial occlusion, functional impairment was seen in the collateral circuit. An immunofluorescence protocol was developed in order to assess the impact of collateral enlargement (arteriogenesis) on smooth muscle phenotype at various time points. Smooth muscle α-actin was used to mark all smooth muscle cells, Ki-67 was used to label proliferating smooth muscle cells, and a fluorescent nuclear stain was used ...
Course Of Illness And The Development Of Vascular Disease In Individuals With Bipolar Disorder, 2011 University of Iowa
Course Of Illness And The Development Of Vascular Disease In Individuals With Bipolar Disorder, Jess G. Fiedorowicz
Theses and Dissertations
For over a century, there have been suggestions of a link between what is currently called bipolar disorder and cardiovascular mortality. In the contemporary epidemiological literature, this risk has been confirmed and approximates twice that expected based on age and gender. To date, however, this information has come primarily from clinical samples, which carry considerable risk of selection bias. The studies contained in this dissertation sought to assess this relationship using methods less vulnerable to selection bias and to determine the role that course of illness and treatments for illness may play in the development of vascular disease. In a ...
A Novel Free Form Femoral Cutting Guide, 2011 University of Tennessee, Knoxville
A Novel Free Form Femoral Cutting Guide, Wesley Andrew Underwood
Knee arthoplasty is a common procedure that requires the removal of damaged bone and cartilage from the distal femur so that a reconstructive implant may be installed. Traditionally, a five planar resection has been accomplished with a universal cutting box and navigated with either metal jigs or optically tracked computer navigation systems. Free form, or curved, resections have been made possible with surgical robots which control the resection pathway and serve as the navigation system. The free form femoral cutting guide serves as a non powered framework to guide a standard surgical drill along an anatomically defined pathway, resulting in ...
Glucose Biosensor Using Electrospun Mn2o3-Ag Nanofibers, 2011 University of Connecticut
Glucose Biosensor Using Electrospun Mn2o3-Ag Nanofibers, Shan Huang
The highly porous Mn2O3-Ag nanofibers were fabricated by a facile two-step procedure (electrospinning and calcination) and then employed as the immobilization matrix for glucose oxidase (GOD) to construct an amperometric glucose biosensor. A notable enhancement of direct electron transfer between GOD and the electrode is observed at the Mn2O3-Ag-GOD modified electrode with a fast electron transfer rate constant. The biosensor also shows fast response to glucose, high sensitivity (40.60 μA×mM-1×cm-2), low detection limit (1.73 µM at S/N=3), low Km,app value and excellent ...
In-Vivo Ct Dosimetry During Virtual Colonoscopy, 2011 The University of Texas Graduate School of Biomedical Sciences at Houston
In-Vivo Ct Dosimetry During Virtual Colonoscopy, Jonathon W. Mueller
UT GSBS Dissertations and Theses (Open Access)
Virtual colonoscopy (VC) is a minimally invasive means for identifying colorectal polyps and colorectal lesions by insufflating a patient’s bowel, applying contrast agent via rectal catheter, and performing multi-detector computed tomography (MDCT) scans. The technique is recommended for colonic health screening by the American Cancer Society but not funded by the Centers for Medicare and Medicaid Services (CMS) partially because of potential risks from radiation exposure. To date, no in‐vivo organ dose measurements have been performed for MDCT scans; thus, the accuracy of any current dose estimates is currently unknown.
In this study, two TLDs were affixed to ...
Applications Of Nanoparticle Image Velocimetry In Nanofluids, 2011 University of Tennessee, Knoxville
Applications Of Nanoparticle Image Velocimetry In Nanofluids, Sara Salim Haque
Particle Image Velocimetry (PIV) is an optical technique used for the visualization of fluid flow. PIV can be combined with other techniques to enhance the analysis of fluid flow. A novel far-field plasmonic resonance enhanced nanoparticle-seeded Particle Image Velocimetry (nPIV) has been demonstrated to measure the velocity in a micro channel. Chemically synthesized silver nanoparticles have been used to seed the flow. By using Discrete Dipole Approximation (DDA), plasmonic resonance enhanced light scattering has been calculated for spherical silver nanoparticles with diameters ranging from 15 nm to 200 nm in two media: water and air. The diffraction-limited plasmonic resonance enhanced ...
Seasonal Initial Concentrations And In-Field Decay Rates Of Escherichia Coli And Bovine Bacteroidetes In Beef Cattle Manure, 2011 University of Tennessee, Knoxville
Seasonal Initial Concentrations And In-Field Decay Rates Of Escherichia Coli And Bovine Bacteroidetes In Beef Cattle Manure, Jiangwei Liu
Eight naturally deposited beef cow manure patties were sampled during summer (July 19 to August 9, 2010), fall (October 26 to November 19, 2010), winter (January 14 to February 18, 2011), and spring (May 5-27, 2011) to determine whether hypothesized seasonal differences existed in the initial concentrations and decay rates of Escherichia coli (E. coli) and bovine Bacteroidetes (BoBac). E. coli concentrations were estimated as culturable colony forming units (CFU) and with a quantitative polymerase chain reaction (qPCR) assay targeting the 23S ribosomal gene. BoBac was quantified with a qPCR assay targeting a 16S ribosomal gene sequence associated with cattle ...
Zein Nanospheres For Gene Delivery, 2011 University of Nebraska-Lincoln
Zein Nanospheres For Gene Delivery, Mary C. Regier
Biological Systems Engineering--Dissertations, Theses, and Student Research
Particulates incorporating DNA provide for protection and sustained release of DNA, and thus are promising candidates for DNA delivery systems. Among the routes of administration for gene delivery, the oral route is perhaps the most appealing as it is associated with patient comfort and compliance and allows for targeting to intestinal targets for therapeutic and vaccination applications. With the goal of realizing the potential of an oral DNA delivery system, zein, a hydrophobic protein from corn that is biocompatible and degraded enzymatically, was investigated. This thesis describes the formulation of zein nanospheres encapsulating DNA by a coacervation technique and their ...
An Inexpensive Method For Creating Paste To Be Used In Paper-Based Microfluidic Devices, 2011 California Polytechnic State University - San Luis Obispo
An Inexpensive Method For Creating Paste To Be Used In Paper-Based Microfluidic Devices, Kirra Marie Sankey
Biomedical Eng/General Eng.
Microfluidic devices are diagnostic tools that are small, portable, and inexpensive. This makes them ideally suited for resource-limited settings. To construct a 3D device, each layer of patterned paper is stacked using double-sided tape with holes corresponding to the pattern. It is necessary to have a paste to fill the holes in the tape and provide a medium for the fluid to wick through. The powder used to make the paste is very expensive and must be bought in bulk (an estimated $1121 - $1231), so it is not convenient for the Biomedical (BMED) department to use. This project was ...