Development Of A Robust Methodology To Obtain And Assess Myogenic Precursor Cells For Their Use In Regenerative Therapies,
2021
California Polytechnic State University, San Luis Obispo
Development Of A Robust Methodology To Obtain And Assess Myogenic Precursor Cells For Their Use In Regenerative Therapies, Ricardo Lasa
Master's Theses
Peripheral arterial occlusive disease (PAOD) is characterized by buildup of atherosclerotic plaque in peripheral arteries that leads to an occlusion that can interrupt the supply of blood to the peripheral tissue, causing downstream tissue ischemia/hypoxia. PAOD is estimated to affect over 200 million patients worldwide. Current surgical revascularization treatments can be effective in about half of the patient population, leading to a significant number of patients with no treatment options beyond pharmacological intervention and lifestyle modification. The decrease in blood flow downstream of the occlusion leads to increased blood pressure gradient in the microvasculature, specifically in vessels that connect arterial …
Development Of An Internet Of Things (Iot) Enabled Novel Wireless Multi Sensor Network For Infield Crop Monitoring,
2021
University of Nebraska-Lincoln
Development Of An Internet Of Things (Iot) Enabled Novel Wireless Multi Sensor Network For Infield Crop Monitoring, Nipuna Chamara
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Multispectral imaging systems on satellite, aerial, and ground platforms are used commonly to monitor in-field crops in precision agriculture by farmers and researchers. Limited spatial and temporal resolution and weather dependence of the data collection are two main disadvantages of these methods. In-field sensor networks can continuously monitor environmental and plant physiological parameters by leveraging low-power computation and long-range communication technologies. We built and tested a novel sensor network equipped with soil moisture, multispectral and RGB imaging sensors in an experimental soybean field at Eastern Nebraska Research and Extension Center, NE, USA. 10 down-looking and 1 up-looking sensor node were …
Managing Vibration Training Safety By Using Knee Flexion Angle And Rating Perceived Exertion,
2021
Chinese Culture University
Managing Vibration Training Safety By Using Knee Flexion Angle And Rating Perceived Exertion, Long-Ren Chuang, Wen-Wen Yang, Po-Ling Chang, Vincent Chiun-Fan Chen, Chiang Liu, Tzyy-Yuang Shiang
Engineering Science Faculty Publications
Whole-body vibration (WBV) is commonly applied in exercise and rehabilitation and its safety issues have been a major concern. Vibration measured using accelerometers can be used to further analyze the vibration transmissibility. Optimal bending angles and rating of perceived exertion (RPE) evaluations have not been sufficiently explored to mitigate the adverse effect. Therefore, the aims of this study were to investigate the effect of various knee flexion angles on the transmissibility to the head and knee, the RPE during WBV exposure, and the link between the transmissibility to the head and the RPE. Sixteen participants randomly performed static squats with …
Deciphering And Engineering An Unprecedented Fungal Biosynthetic Pathway For Expanded Chemical Diversity In Flavonoids,
2021
Utah State University
Deciphering And Engineering An Unprecedented Fungal Biosynthetic Pathway For Expanded Chemical Diversity In Flavonoids, Jixun Zhan
Funded Research Records
No abstract provided.
Zein And Lignin-Based Nanoparticles As Delivery Systems: Pesticide Release And Nanoparticle Health Impact On Soybean Plants,
2021
Louisiana State University and Agricultural and Mechanical College
Zein And Lignin-Based Nanoparticles As Delivery Systems: Pesticide Release And Nanoparticle Health Impact On Soybean Plants, Fallon Polette Salinas Gonzalez
LSU Doctoral Dissertations
This research examined the effect of biodegradable, polymeric, lignin-based nanoparticles (LNPs, 113.8±3.4, negatively charged) and zein nanoparticles (ZNP, 141.6±3.9, positively charged) on soybean plant health. The LNPs were synthesized from lignin, covalently linked to poly(lactic-co-glycolic) acid by emulsion evaporation. ZNPs were synthesized by nanoprecipitation. Soybeans grown hydroponically were treated with three concentrations (0.02, 0.2, and 2 mg/ml) of NPs at 28 days after germination. The effect of ZNPs and LNPs on plant health was determined through analysis of root and stem length, chlorophyll concentration, dry biomass of roots and stem, as well as carbon, nitrogen, and micronutrient absorption after 1, …
Development Of The Cell-Free Protein Biomanufacturing Platform For The Therapeutic Human Protein Production And The Topical Delivery Applications Using The Nanosized Hydrogel System,
2021
Louisiana State University
Development Of The Cell-Free Protein Biomanufacturing Platform For The Therapeutic Human Protein Production And The Topical Delivery Applications Using The Nanosized Hydrogel System, Jeehye Kim
LSU Doctoral Dissertations
Proteins are highly structural polymers, performing an enormous range of roles within organisms. Unlike synthetic polymers, proteins have a significant hierarchical ordering that can be disrupted by many environmental factors, which limits the scale-up manufacturing capability as well as the utilization of proteins as therapeutic agents. In this study, the Cell-Free Protein Synthesis (CFPS) system was developed to enhance the production capacity and applications of the therapeutic proteins. The CFPS is an advanced biosynthetic and genetic-engineered system. It enables the efficient production of proteins when the conventional cell-based protein synthesis systems cannot be applied, and when sophisticated control is required …
Injectable Gelatin-Silk Fibroin Composite Hydrogels For In Situ Cell Encapsulation,
2021
The University of New Hampshire
Injectable Gelatin-Silk Fibroin Composite Hydrogels For In Situ Cell Encapsulation, Ryann D. Boudreau
Honors Theses and Capstones
Hydrogels are widely used tools for tissue engineering and regenerative medicine. Characterized as biofunctional, water-based polymer matrices with tunable mechanical properties, hydrogels have promising but limited applications in biomedical engineering, due to poor and static matrix strength. Here we plan to rectify this issue by introducing a new hydrogel made from a composite of gelatin and silk fibroin crosslinked by microbial transglutaminase (mTG) instantly and beta sheet formation gradually, respectively. This interpenetrating network (IPN) shows enhanced mechanical stiffness and strength compared to gelatin hydrogels, and is capable of encapsulating human cells with high viability demonstrated by the encapsulation of human …
Correlating With Local Variation In Collagen Level And Mechanical Properties Of The Heart Tissues,
2021
University of Denver
Correlating With Local Variation In Collagen Level And Mechanical Properties Of The Heart Tissues, Talayeh Tavangar
Electronic Theses and Dissertations
The determination of the myocardium's tissue properties is essential in constructing finite element (FE) models of the heart. To obtain accurate results, especially for functionally modeling the heart, we must determine the tissue properties in-vivo. The calculation of the heart tissue properties remains a challenging area as it is categorized as a heterogeneous, anisotropic, nonlinear soft tissue that undergoes large deformation. In this work, we tried to introduce and evaluated a finite element method to determine the mechanical properties of the cardiac tissue. The introduced method combined a finite element modeling with the experimentally obtained images from the left and …
Two-Dimensional & Three-Dimensional Microarray Cell Culture Using Elastomeric Assembly Substrates,
2021
University of Nebraska - Lincoln
Two-Dimensional & Three-Dimensional Microarray Cell Culture Using Elastomeric Assembly Substrates, Angel Olivera-Torres
Honors Theses
Tissue engineering and regenerative medicine represent the collection of all engineering disciplines brought together for the common goal of developing novel ways of growing tissues and organs in the laboratory. Efforts have made it possible to replicate or induce growth of 2D structures in the human body like skin, but the clinical need for on-demand solid organs has yet to be met due to lack of understanding of the variables responsible for organogenesis. Cell-colony heterogeneity, 3D-cellular architecture, bioactive molecules, and crosstalk communication between parenchymal cell populations need to be further investigated, and high-throughput technologies can rapidly increase the rate at …
Co2 Biocapture By Scenedesmus Sp. Grown In Industrial Wastewater,
2021
Tecnologico de Monterrey
Co2 Biocapture By Scenedesmus Sp. Grown In Industrial Wastewater, Itzel Y. López-Pacheco, Eduardo Israel Castillo-Vacas, Lizbeth Castañeda-Hernández, Angie Gradiz-Menjivar, Laura Isabel Rodas-Zuluaga, Carlos Castillo-Zacarías, Juan Eduardo Sosa-Hernández, Damià Barceló, Hafiz M.N. Iqbal, Roberto Parra-Saldívar
Panhandle Research and Extension Center
Greenhouse gases (GHG) emissions are widely related to climate change, triggering several environmental problems of global concern and producing environmental, social, and economic negative impacts. Therefore, global research seeks to mitigate greenhouse gas emissions. On the other hand, the use of wastes under a circular economy scheme generates subproducts from the range of high to medium-value, representing away to help sustainable development. Therefore, the use of wastewater as a culture medium to grow microalgae strains that biocapture environmental CO2, is a proposal with high potential to reduce the GHG presence in the environment. In this work, Scenedesmus sp. …
Development Of Light Actuated Chemical Delivery Platform On A 2-D Array Of Micropore Structure,
2021
CUNY City College
Development Of Light Actuated Chemical Delivery Platform On A 2-D Array Of Micropore Structure, Hojjat Rostami Azmand, Hojjat Rostami Azmand
Dissertations and Theses
Localized chemical delivery plays an essential role in the fundamental information transfers within biological systems. Thus, the ability to mimic the natural chemical signal modulation would provide significant contributions to understand the functional signaling pathway of biological cells and develop new prosthetic devices for neurological disorders. In this paper, we demonstrate a light-controlled hydrogel platform that can be used for localized chemical delivery in a high spatial resolution. By utilizing the photothermal behavior of graphene-hydrogel composites confined within micron-sized fluidic channels, patterned light illumination creates the parallel and independent actuation of chemical release in a group of fluidic ports. The …
2-Ns Electrostimulation Of Ca2+ Influx Into Chromaffin Cells: Rapid Modulation By Field Reversal,
2021
Old Dominion University
2-Ns Electrostimulation Of Ca2+ Influx Into Chromaffin Cells: Rapid Modulation By Field Reversal, Josette Zaklit, Gale L. Craviso, Normand Leblanc, P. Thomas Vernier, Esin B. Sözer
Bioelectrics Publications
Cellular effects of nanosecond pulsed electric field exposures can be attenuated by an electric field reversal, a phenomenon called bipolar pulse cancellation. Our investigations of this phenomenon in neuroendocrine adrenal chromaffin cells show that a single 2 ns, 16 MV/m unipolar pulse elicited a rapid, transient rise in intracellular Ca2+ levels due to Ca2+ influx through voltage-gated calcium channels. The response was eliminated by a 2 ns bipolar pulse with positive and negative phases of equal duration and amplitude, and fully restored (unipolar-equivalent response) when the delay between each phase of the bipolar pulse was 30 ns. Longer …
Tibial Strains And Tray-Bone Micromotions After Total Knee Arthroplasty: Computational Studies Evaluating The Tibial Fixation,
2021
University of Denver
Tibial Strains And Tray-Bone Micromotions After Total Knee Arthroplasty: Computational Studies Evaluating The Tibial Fixation, Huizhou Yang
Electronic Theses and Dissertations
Cemented and cementless fixation in total knee arthroplasty (TKA) have been successfully used for decades. As the number of younger and more active patients treated with TKA continues to increase, long-term implant survivorship is of increasing importance. One of the most common complications and hence the reason for revision is mechanical loosening (23.1% of all revised TKA). The loosening mechanisms have been proposed for different fixation types. For cemented fixation, bone remodeling after surgery is regulated by the changes in strain energy density (SED). The recruitment of osteoclasts and osteoblasts is controlled by SED-related signals. Insufficient stimuli can promote bone …
Ecg Monitoring In Athletes,
2021
The University of Akron
Ecg Monitoring In Athletes, Jalal Jwayyed, Kelly Purgason, Joseph Linder, Annamarie Alfery, Silvia Furman
Williams Honors College, Honors Research Projects
Athletes and medical personnel need reliable methods to monitor vital signs and detect potentially life-threatening issues before they occur. Electrocardiograph (ECG) monitors are currently the best method of detecting heart-related health issues. Current ECG monitors worn by athletes are difficult to use and lack the ability to track ECGs in real time. Through research and interviews with various clinicians, further problems have been identified that include poor electrode adhesion, and a lack of compatibility with the equipment worn by athletes. Our team proposes to develop solutions that will improve performance and comfort associated with ECG monitors for athletic applications. This …
Blast Shock-Wave Characterization In Experimental Shock Tubes,
2020
New Jersey Institute of Technology
Blast Shock-Wave Characterization In Experimental Shock Tubes, Sudeepto Kahali
Dissertations
Blast-induced traumatic brain injuries have affected U.S. soldiers deployed for extended periods in the gulf and Afghanistan wars. To identify the biomechanical and biochemical mechanisms of injury, critical in the identification of diagnostic and therapeutic tools, compressed gas-driven shock tubes are used by investigators to study shockwave-animal specimen interactions and its biological consequences. However, shock tubes are designed and operated in a variety of geometry with a range of process parameters, and the quality of shock wave characteristics relevant to field conditions and therefore the study of blast-induced traumatic brain injuries suffered by soldiers is affected by those conditions. Lab-to-lab …
Micro-Physiological Models To Mimic Mucosal Barrier Complexity Of The Human Intestine In Vitro,
2020
University of Massachusetts Amherst
Micro-Physiological Models To Mimic Mucosal Barrier Complexity Of The Human Intestine In Vitro, Abhinav Sharma
Doctoral Dissertations
The mucosal barrier in the intestine is vital to maintain selective absorption of nutrients while protecting internal tissues and maintaining symbiotic relationship with luminal microbiota. This bio-barrier consists of a cellular epithelial barrier and an acellular mucus barrier. Secreted mucus regulates barrier function via in situ biochemical and biophysical interaction with luminal content that continually evolves during digestion and absorption. Increasing evidence suggests that a mucus barrier is indispensable to maintain homeostasis in the gastrointestinal tract. However, the importance of mucus barrier is largely underrated for in vitro mucosal tissue modeling. The major gap is the lack of experimental material …
Engineering Mesothelin-Binding Proteins As Targeted Cancer Diagnostics And Therapeutics,
2020
University of Massachusetts Amherst
Engineering Mesothelin-Binding Proteins As Targeted Cancer Diagnostics And Therapeutics, Allison Rita Sirois
Doctoral Dissertations
Cancer is a significant global health concern; and traditional therapies, including chemotherapeutics, are often simultaneously toxic yet ineffective. There is a critical need to develop targeted cancer therapeutics which specifically inhibit molecules or molecular pathways essential for tumor growth and maintenance. Furthermore, a targeted therapy is only effective when a patient's tumor expresses the molecular target; therefore, companion diagnostics, including molecular imaging agents, are a necessary counterpart of targeted therapies. Mesothelin (MSLN) is a cell surface protein overexpressed in numerous cancers, including triple-negative breast, pancreatic, ovarian, liver, and lung, with limited expression in normal tissues. Aberrant MSLN expression promotes tumor …
Intensification Of Clostridium Pasteurianum Fermentation Producing N-Butanol From Glycerol Using Microfiltration Cell Recycle,
2020
The University of Western Ontario
Intensification Of Clostridium Pasteurianum Fermentation Producing N-Butanol From Glycerol Using Microfiltration Cell Recycle, Colin W. Couper
Electronic Thesis and Dissertation Repository
This work demonstrates lab scale intensification of the fermentation of glycerol to 1-butanol using Clostridum pasteurianum, starting with simulation and comparison of different cell recycle arrangements, development of a cell recycle apparatus with an existing bioreactor, and demonstration of fermentation with the final system. Fermentations performed with the completed system showed that the cell recycle system was not significantly inhibitory to fermentation, and achieved a maximum apparent cell dry weight of 3.14g/L and a maximum butanol productivity of 1.16g/Lh.
Functional Complementation Of The Ppgcn4 And Ppnhx2 Genes In Arabidopsis Thaliana To Study Salt Tolerance,
2020
Utah State University
Functional Complementation Of The Ppgcn4 And Ppnhx2 Genes In Arabidopsis Thaliana To Study Salt Tolerance, Amanda Moravek
Fall Student Research Symposium 2020
Climate change induces unexpected weather and causes abiotic and biotic stresses in plants. These stresses negatively affect crop growth and production. Additionally, a steady increase in the world population has been leading to higher food demand. Therefore, the development of more stress-resilient crops is essential to combat these problems. One such stress is a salinity that affects crop production. Almond is a salt-sensitive crop, so there is a need to identify salt-tolerant almond rootstocks. AtGCN4 is a novel gene that was identified in Arabidopsis thaliana to play a significant role in host-pathogen interaction and drought tolerance when overexpressed. The preliminary …
Dogs In Ancient Egypt,
2020
Utah State University
Dogs In Ancient Egypt, Ella Olson
Fall Student Research Symposium 2020
The Ancient Egyptians are infamous for their love of cats, but what about dogs? It turns out that dogs were equally loved and praised in Egyptian culture. A large part of this comes from the religious significance of dogs- they are connected to the afterlife through Anubis, the god of the dead, and were thought to act as companions and guides to humans in the afterlife. Anubis is an interesting god to look at because he's one of the oldest and most respected gods. In today's terms, we refer to him as having a jackal head, but the Egyptians considered …
