Open Access. Powered by Scholars. Published by Universities.®

Biological Engineering Commons

Open Access. Powered by Scholars. Published by Universities.®

1192 Full-Text Articles 1804 Authors 560140 Downloads 47 Institutions

All Articles in Biological Engineering

Faceted Search

1192 full-text articles. Page 6 of 32.

Development Of A Glucose-Powered Biobattery For Implantation And Use In Humans, Carson Sparks, Cody Maughan, Lucas Smith, Carson Sparks 2016 Utah State University

Development Of A Glucose-Powered Biobattery For Implantation And Use In Humans, Carson Sparks, Cody Maughan, Lucas Smith, Carson Sparks

Research on Capitol Hill

With current demands for implantable electrical devices increasing, the need for a more stable and biocompatible source of power is becoming increasingly necessary. Several battery types and materials were evaluated. Ultimately, an abiotic biobattery was designed with the goal of implantation in the human body. Nafion, single-walled carbon nanotubes (SWCNTs), and gold were used to create an abiotic biobattery that is powered by glucose.

The SWCNTs were used to create the cathode, the gold was used to fabricate the anode, and the Nafion acted as the separator between the cathode and anode. A thin Nafion membrane was evaluated for overlaying ...


Improved Production Of Promising Antioxidant, Resveratrol, In Escherichia Coli, Chad Skidmore 2016 Utah State University

Improved Production Of Promising Antioxidant, Resveratrol, In Escherichia Coli, Chad Skidmore

Research on Capitol Hill

Resveratrol is a promising antioxidant natural product. Studies have shown that it is effective against heart disease, cancer, Alzheimer's disease, diabetes, and harmful UV rays. This health-benefiting molecule is present in plants such as peanuts, berries, and the skin of red grapes.

A growth time of 10 months makes Japanese knotweed an impractical source of resveratrol. A more efficient way to produce resveratrol has been found by using E. coli as tiny biological factories.


Beyond The Fiber: Novel Spider Silk Coatings And Adhesives, Danielle A. Gaztambide, Breton A. Day 2016 Utah State University

Beyond The Fiber: Novel Spider Silk Coatings And Adhesives, Danielle A. Gaztambide, Breton A. Day

Research on Capitol Hill

Natural spider silks have long been recognized for their combination of incredible strength and elasticity. Spider silk is more elastic than nylon, tougher than Kevlar, and stronger than steel by weight. Due to an inability to farm spiders, much work has been done to produce spider silks in transgenic hosts for large -scale production. Our work was done using recombinant spider silk proteins produced in transgenic goats and the bacteria E. coli.

More recently spider silks have also been recognized for their biocompatibility and lack of immunogenicity. Spider silks' incredible strength and ability to be implanted safely within the body ...


Zeta Potential: Key To Harvesting Algae For Biofuels And Bioproducts, Celeste Hancock, Michael Flores 2016 Utah State University

Zeta Potential: Key To Harvesting Algae For Biofuels And Bioproducts, Celeste Hancock, Michael Flores

Research on Capitol Hill

  • Algae is an effective and sustainable resource for creating a broad spectrum of bioproducts.
  • Scientists have found it challenging to harvest algae due to the difficulty of collecting algae when in an aqueous solution such as wastewater.
  • Rotating Algal Biofilm Reactors (RABRs) coated with carbon nanotubes have proved effective. The RABR floats in an aqueous environment and attracts charged algal particles in suspension.
  • The tendency for algae to favor suspension over coagulation occurs only when particles of algae are sufficiently charged. This charge can be measured by analyzing the electric potential at the interface between the surface of a particle ...


Modeling Alveolar Epithelial Cell Behavior In Spatially Designed Hydrogel Microenvironments, Katherine Jean Reeder Lewis 2016 University of Colorado Boulder

Modeling Alveolar Epithelial Cell Behavior In Spatially Designed Hydrogel Microenvironments, Katherine Jean Reeder Lewis

Chemical & Biological Engineering Graduate Theses & Dissertations

The alveolar epithelium consists of two cell phenotypes, elongated alveolar type I cells (AT1) and rounded alveolar type II cells (ATII), and exists in a complex three-dimensional environment as a polarized cell layer attached to a thin basement membrane and enclosing a roughly spherical lumen. Closely surrounding the alveolar cysts are capillary endothelial cells as well as interstitial pulmonary fibroblasts. Many factors are thought to influence alveolar epithelial cell differentiation during lung development and wound repair, including physical and biochemical signals from the extracellular matrix (ECM), and paracrine signals from the surrounding mesenchyme. In particular, disrupted signaling between the alveolar ...


Arakniprint: 3d Printing Of Synthetic Spider Silk To Produce Biocompatible And Resorbable Biomaterials, Ashley Ruben, Brianne Bell, Chase Spencer, Craig Soelberg, Dan Gil, Thomas Harris, Richard Decker, Timothy A. Taylor, Randolph V. Lewis 2016 Utah State University

Arakniprint: 3d Printing Of Synthetic Spider Silk To Produce Biocompatible And Resorbable Biomaterials, Ashley Ruben, Brianne Bell, Chase Spencer, Craig Soelberg, Dan Gil, Thomas Harris, Richard Decker, Timothy A. Taylor, Randolph V. Lewis

UCUR

No abstract provided.


Rational Design Of Rama-Labeled Nanoparticles For A Dual-Modaility, Light Scattering Immunoassay On A Polystyrene Seubstrate, Nathan D. Israelsen, Donald Wooley, Cynthia Hanson, Elizabeth Vargis 2016 Utah State University

Rational Design Of Rama-Labeled Nanoparticles For A Dual-Modaility, Light Scattering Immunoassay On A Polystyrene Seubstrate, Nathan D. Israelsen, Donald Wooley, Cynthia Hanson, Elizabeth Vargis

Biological Engineering Faculty Publications

Background: Surface-enhanced Raman scattering (SERS) is a powerful light scattering technique that can be used for sensitive immunoassay development and cell labeling. A major obstacle to using SERS is the complexity of fabricating SERS probes since they require nanoscale characterization and optical uniformity. The light scattering response of SERS probes may also be modulated by the substrate used for SERS analysis. A typical SERS substrate such as quartz can be expensive. Polystyrene is a cheaper substrate option but can decrease the SERS response due to interfering Raman emission peaks and high background fluorescence. The goal of this research is to ...


Microbubble Assisted Polyhydroxybutyrate Production In Escherichia Coli, Kadriye Innan, Fulya Ay Sal, Asif Rahman, Ryan J. Putman, Foster A. Agblevor, Charles D. Miller 2016 Utah State University

Microbubble Assisted Polyhydroxybutyrate Production In Escherichia Coli, Kadriye Innan, Fulya Ay Sal, Asif Rahman, Ryan J. Putman, Foster A. Agblevor, Charles D. Miller

Biological Engineering Faculty Publications

Background

One of the potential limitations of large scale aerobic Escherichia coli fermentation is the need for increased dissolved oxygen for culture growth and bioproduct generation. As culture density increases the poor solubility of oxygen in water becomes one of the limiting factors for cell growth and product formation. A potential solution is to use a microbubble dispersion (MBD) generating device to reduce the diameter and increase the surface area of sparged bubbles in the fermentor. In this study, a recombinantE. coli strain was used to produce polyhydroxybutyrate (PHB) under conventional and MBD aerobic fermentation conditions.

Results

In conventional ...


Automated Solid-Substrate Cultivation Of The Anaerobic Bacterium Clostridium Thermocellum, Mathew J. Ruwaya 2016 University of Kentucky

Automated Solid-Substrate Cultivation Of The Anaerobic Bacterium Clostridium Thermocellum, Mathew J. Ruwaya

Theses and Dissertations--Biosystems and Agricultural Engineering

The organism Clostridium thermocellum grows on cellulosic substrates and produces ethanol, acetate, lactate, formic acid, and CO2. The organic acids produced alter the growth environment in which the bacteria grows and ultimately inhibit bacterial growth. One method which has been used successfully to maintain the system at acceptable growth conditions is to intermittently flush out the spent media and metabolic products and replace with new fermentation media. Our goal was to design and build an automated system that will automatically flush the spent media from the growing culture and resupply new media without manual intervention. An automated control system ...


Retargeting The Clostridium Botulinum C2 Toxin To The Neuronal Cytosol, Benjamin J. Pavlik, Elizabeth J. Hruska, Kevin Van Cott, Paul Helmuth Blum 2016 University of Nebraska- Lincoln

Retargeting The Clostridium Botulinum C2 Toxin To The Neuronal Cytosol, Benjamin J. Pavlik, Elizabeth J. Hruska, Kevin Van Cott, Paul Helmuth Blum

Chemical and Biomolecular Engineering -- All Faculty Papers

Many biological toxins are known to attack specific cell types, delivering their enzymatic payloads to the cytosol. This process can be manipulated by molecular engineering of chimeric toxins. Using toxins with naturally unlinked components as a starting point is advantageous because it allows for the development of payloads separately from the binding/translocation components. Here the Clostridium botulinum C2 binding/translocation domain was retargeted to neural cell populations by deleting its non-specific binding domain and replacing it with a C. botulinum neurotoxin binding domain. This fusion protein was used to deliver fluorescently labeled payloads to Neuro-2a cells. Intracellular delivery was ...


Bioengineered Platforms To Study Carcinoma Cell Response To Drug Treatment, Thuy V. Nguyen 2016 University of Massachusetts Amherst

Bioengineered Platforms To Study Carcinoma Cell Response To Drug Treatment, Thuy V. Nguyen

Doctoral Dissertations

The tumor extracellular matrix (ECM) plays an important role in facilitating tumor growth and mediating tumor cells' resistance to drugs. However, during drug development, potential chemotherapeutics are screened in plastic plates, which lack relevant ECM physicochemical cues. In order to improve drug development process, this dissertation includes the development of relevant 2D and 3D biomaterial systems that can be used to study carcinoma cell response to drug treatment.

A novel poly(ethylene glycol)-phosphorylcholine (PEG-PC) high-throughput biomaterial platform was developed to study how the ECM mechanochemical properties affect cancer cells' response to drug. The PEG-PC biomaterial is optically transparent, has ...


Accounting For Host Cell Protein Behavior In Anion-Exchange Chromatography, Ryan K. Swanson, Ruo Xu, Daniel S. Nettleton, Charles Glatz 2016 Iowa State University

Accounting For Host Cell Protein Behavior In Anion-Exchange Chromatography, Ryan K. Swanson, Ruo Xu, Daniel S. Nettleton, Charles Glatz

Chemical and Biological Engineering Publications

Host cell proteins (HCP) are a problematic set of impurities in downstream processing (DSP) as they behave most similarly to the target protein during separation. Approaching DSP with the knowledge of HCP separation behavior would be beneficial for the production of high purity recombinant biologics. Therefore, this work was aimed at characterizing the separation behavior of complex mixtures of HCP during a commonly used method: anion-exchange chromatography (AEX). An additional goal was to evaluate the performance of a statistical methodology, based on the characterization data, as a tool for predicting protein separation behavior. Aqueous two-phase partitioning followed by two-dimensional electrophoresis ...


Identifying Factors Of Microparticles Modified With Arginine Derivatives That Induce Phenotypic Shifts In Macrophages, Alexander J. Dunn-Sale, Kaitlin M. Bratlie 2016 Iowa State University

Identifying Factors Of Microparticles Modified With Arginine Derivatives That Induce Phenotypic Shifts In Macrophages, Alexander J. Dunn-Sale, Kaitlin M. Bratlie

Materials Science and Engineering Publications

Macrophages are key players in the progression of many diseases, ranging from rheumatoid arthritis to cancer. Drug delivery systems have the potential not only to transport payloads to diseased tissue but also to influence cell behavior. Here, poly(N-isopropylacrylamide-co-acrylic acid) (pNIPAm-co-AAc) microparticles were modified with 14 different arginine derivatives. These particles were then incubated with interleukin-4 or lipopolysaccharide-stimulated macrophages or naïve macrophages (RAW 264.7). The phenotypic state of the macrophages was assessed by measuring arginase activity, tumor necrosis factor-α (TNF-α) secretion, and nitrite production. Partial least-squares analysis revealed material properties and descriptors that shifted the macrophage phenotype for the ...


Combinatorial Hydrogel Library Enables Identification Of Materials That Mitigate The Foreign Body Response In Primates, Arturo J. Vegas, Kaitlin M. Bratlie, et al. 2016 Massachusetts Institute of Technology

Combinatorial Hydrogel Library Enables Identification Of Materials That Mitigate The Foreign Body Response In Primates, Arturo J. Vegas, Kaitlin M. Bratlie, Et Al.

Materials Science and Engineering Publications

The foreign body response is an immune-mediated reaction that can lead to the failure of implanted medical devices and discomfort for the recipient16. There is a critical need for biomaterials that overcome this key challenge in the development of medical devices. Here we use a combinatorial approach for covalent chemical modification to generate a large library of variants of one of the most widely used hydrogel biomaterials, alginate. We evaluated the materials in vivo and identified three triazole-containing analogs that substantially reduce foreign body reactions in both rodents and, for at least 6 months, in non-human primates. The ...


Optimization Of Micropillar Sequences For Fluid Flow Sculpting, Daniel Stoecklein, Chueh-Yu Wu, Donghyuk Kim, Dino Di Carlo, Baskar Ganapathysubramanian 2016 Iowa State University

Optimization Of Micropillar Sequences For Fluid Flow Sculpting, Daniel Stoecklein, Chueh-Yu Wu, Donghyuk Kim, Dino Di Carlo, Baskar Ganapathysubramanian

Mechanical Engineering Publications

Inertial fluid flow deformation around pillars in a microchannel is a new method for controlling fluid flow. Sequences of pillars have been shown to produce a rich phase space with a wide variety of flow transformations. Previous work has successfully demonstrated manual design of pillar sequences to achieve desired transformations of the flow cross section, with experimental validation. However, such a method is not ideal for seeking out complex sculpted shapes as the search space quickly becomes too large for efficient manual discovery.We explore fast, automated optimization methods to solve this problem. We formulate the inertial flow physics in ...


Inhibition Of Bacterial Growth And Prevention Of Bacterial Adhesion With Localized Nitric Oxide Delivery, Julia Osborne 2016 Michigan Technological University

Inhibition Of Bacterial Growth And Prevention Of Bacterial Adhesion With Localized Nitric Oxide Delivery, Julia Osborne

Dissertations, Master's Theses and Master's Reports

Bacterial infections continue to be a problem at the site of an indwelling medical device, and over the years, various bacterial strains have become more resistant to current antibiotic treatments. Bacterial infection at an indwelling medical device can be dangerous and affect the performance of the medical device which can ultimately lead to the failure of the device due to bacterial resistance to treatment.

Nitric Oxide (NO) has been shown to possess antibacterial properties to prevent and inhibit bacterial growth. NO releasing coatings on indwelling medical devices could provide a reduction in bacterial infections that occur at the device site ...


Use Of Lidar In The Design Of Grassed Waterways: Case Study In Agricultural Management In Oklahoma, Annette Sparks 2016 Michigan Technological University

Use Of Lidar In The Design Of Grassed Waterways: Case Study In Agricultural Management In Oklahoma, Annette Sparks

Dissertations, Master's Theses and Master's Reports

Before the advent of Light Detection and Ranging (LiDAR) technology, farm-level infrastructure for sustainable agriculture was designed using topographic maps. LiDAR is a remote sensing method whereby reflected laser pulses are measured to generate high-resolution 3D images of the terrain. This report compares these two methods in the design of grassed waterways for flood drainage and evaluates how the quantitative difference between the two methods can affect the overall hydrologic design. The benefits of LiDAR are expected to be higher accuracy and precision in design, as well as greater reproducibility or consistency, regardless of the designer. To carry out this ...


Investigating Biosynthetic Steps Of An Angucycline Antifungal, S. Gabrielle Gladstone 2016 Utah State University

Investigating Biosynthetic Steps Of An Angucycline Antifungal, S. Gabrielle Gladstone

All Graduate Theses and Dissertations

From the bacterium Streptomyces sp. SCC-2136 (ATCC 55186), two angucycline natural products are produced, designated Sch 47554 and Sch 47555. These compounds are produced through a type II polyketide biosynthetic pathway. The early biosynthetic steps to these molecules were confirmed. These include the minimal polyketide synthase (PKS), the C-9 keteoreductase, the first-ring aromatase, the subsequent ring cyclase, and two oxygenases. Also confirmed were the biosynthetic genes responsible for production of the first amicetose moiety, as well as the glycosyltransferase that creates a C-glycosidic bond between the angucyclic scaffold and the amicetose moiety, In confirming these patways, two new natural products ...


Engineering Of Polyketide Biosynthetic Pathways For Bioactive Molecules, Siyuan Wang 2016 Utah State University

Engineering Of Polyketide Biosynthetic Pathways For Bioactive Molecules, Siyuan Wang

All Graduate Theses and Dissertations

Polyketides are a large group of structurally diverse natural products that have shown a variety of biological activities. These molecules are synthesized by polyketide synthases (PKSs). PKSs are classified into three types based on their sequence, primary structure, and catalytic mechanism. Because of the bioactivities of polyketide natural products, this study is focused on the engineering of PKS pathways for efficient production of useful bioactive molecules or structural modification to create new molecules for drug development.

One goal of this research is to create an efficient method to produce pharmaceutically important molecules. Seven biosynthetic genes from plants and bacteria were ...


Towards The Development Of A Wearable Tremor Suppression Glove, Yue Zhou 2015 The University of Western Ontario

Towards The Development Of A Wearable Tremor Suppression Glove, Yue Zhou

Electronic Thesis and Dissertation Repository

Patients diagnosed with Parkinson’s disease (PD) often associate with tremor. Among other symptoms of PD, tremor is the most aggressive symptom and it is difficult to control with traditional treatments. This thesis presents the assessment of Parkinsonian hand tremor in both the time domain and the frequency domain, the performance of a tremor estimator using different tremor models, and the development of a novel mechatronic transmission system for a wearable tremor suppression device. This transmission system functions as a mechatronic splitter that allows a single power source to support multiple independent applications. Unique features of this transmission system include ...


Digital Commons powered by bepress