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Transcriptomics To Develop Biochemical Network Models In Cyanobacteria, Bridget E. Hegarty, Jordan Peccia, Ratanachat Racharaks 2018 Yale University

Transcriptomics To Develop Biochemical Network Models In Cyanobacteria, Bridget E. Hegarty, Jordan Peccia, Ratanachat Racharaks

Yale Day of Data

Through targeted genetic manipulations guided by network modeling, we will create a flexible, cyanobacteria-based platform for the production of biofuel-precursors and valuable chemical products. To build gene-metabolite predictive models, we have characterized Synecococcus elongatus sp. UTEX 2973’s (henceforth, UTEX 2973) gene expression and metabolite production under a number of environmental conditions.


Kinetic Modeling Of Corn Fermentation With S. Cerevisiae Using A Variable Temperature Strategy, Augusto C. M. Souza, Mohammad Mousaviraad, Kenneth O. M. Mapoka, Kurt A. Rosentrater 2018 Iowa State University

Kinetic Modeling Of Corn Fermentation With S. Cerevisiae Using A Variable Temperature Strategy, Augusto C. M. Souza, Mohammad Mousaviraad, Kenneth O. M. Mapoka, Kurt A. Rosentrater

Agricultural and Biosystems Engineering Publications

While fermentation is usually done at a fixed temperature, in this study, the effect of having a controlled variable temperature was analyzed. A nonlinear system was used to model batch ethanol fermentation, using corn as substrate and the yeast Saccharomyces cerevisiae, at five different fixed and controlled variable temperatures. The lower temperatures presented higher ethanol yields but took a longer time to reach equilibrium. Higher temperatures had higher initial growth rates, but the decay of yeast cells was faster compared to the lower temperatures. However, in a controlled variable temperature model, the temperature decreased with time with the initial value ...


Dynamic Classification Of Moisture Stress Using Canopy And Leaf Temperature Responses To A Step Changes Of Incident Radiation, Erin E. Stevens, George E. Meyer, Ellen T. Paparozzi 2018 University of Nebraska-Lincoln

Dynamic Classification Of Moisture Stress Using Canopy And Leaf Temperature Responses To A Step Changes Of Incident Radiation, Erin E. Stevens, George E. Meyer, Ellen T. Paparozzi

Honors Theses, University of Nebraska-Lincoln

Environmental conditions affect plant productivity and understanding how plants respond to drought stress can be measured in different ways. This study focused on measuring leaf response time to induced water stress. Leaf response time to a step increase and step decrease in radiation was computed for four species of well-watered and water-stressed plants in a controlled environment. The canopy temperature was measured with an infrared thermometer and a thermal imaging camera. Thermal images were analyzed to determine the average temperature of a selected single, unobstructed leaf at the top of the canopy. Both the canopy response time and the single ...


Tumor-Targeted Double Emulsions For Ultrasound-Triggered Delivery Of Molecular Therapeutics., Connor S Centner 2018 University of Louisville

Tumor-Targeted Double Emulsions For Ultrasound-Triggered Delivery Of Molecular Therapeutics., Connor S Centner

Electronic Theses and Dissertations

Cancer is the second leading cause of death in the United States, with 1.74 million new cancer cases diagnosed and 610,000 cancer deaths expected in 2018 alone. Current treatments often result in negative systemic effects and ineffective treatment of the tumor. Drug delivery vehicles have been developed for more effective local delivery methods, but many drug delivery vehicles lack spatial and temporal control. Targeted double emulsions are a new class of drug delivery vehicles which present a promising option for a high payload and controlled delivery. The purpose of our project was to develop and characterize an aptamer-chelated ...


3d Tissue Engineering, An Emerging Technique For Pharmaceutical Research, Gregory Jensen, Christian Morrill, Yu Huang 2018 Utah State University

3d Tissue Engineering, An Emerging Technique For Pharmaceutical Research, Gregory Jensen, Christian Morrill, Yu Huang

Biological Engineering Faculty Publications

Tissue engineering and the tissue engineering model have shown promise in improving methods of drug delivery, drug action, and drug discovery in pharmaceutical research for the attenuation of the central nervous system inflammatory response. Such inflammation contributes to the lack of regenerative ability of neural cells, as well as the temporary and permanent loss of function associated with neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and traumatic brain injury. This review is focused specifically on the recent advances in the tissue engineering model made by altering scaffold biophysical and biochemical properties for use in the treatment of ...


Polymeric Peptide Mimics For Protein Delivery, Coralie Backlund 2018 University of Massachusetts Amherst

Polymeric Peptide Mimics For Protein Delivery, Coralie Backlund

Doctoral Dissertations

The plasma membrane is a major obstacle in the development and use of biomacromolecules for intracellular applications. Consequently, proteins with intracellular targets represent an enormous, yet under studied avenue for therapeutics. Extended research has aimed at facilitating intracellular delivery of exogenous proteins using protein transduction domains (PTDs), which allow transport of bioactive molecules into cells. Synthetic polymers, inspired by PTDs, provide a well-controlled platform to vary molecular architecture for structure activity relationship studies. Specifically, this thesis focuses on the use of ring-opening metathesis, a facile and efficient polymerization technique, through which we can vary structural parameters to optimize delivery of ...


Efficient One‑Step Fusion Pcr Based On Dual‑Asymmetric Primers And Two‑Step Annealing, Yilan Liu, Jinjin Chen, Anders Thygesen 2018 University of Nebraska-Lincoln

Efficient One‑Step Fusion Pcr Based On Dual‑Asymmetric Primers And Two‑Step Annealing, Yilan Liu, Jinjin Chen, Anders Thygesen

Chemical and Biomolecular Engineering -- All Faculty Papers

Gene splicing by fusion PCR is a versatile and widely used methodology, especially in synthetic biology. We here describe a rapid method for splicing two fragments by one-round fusion PCR with a dual-asymmetric primers and two-step annealing (ODT) method. During the process, the asymmetric intermediate fragments were generated in the early stage. Thereafter, they were hybridized in the subsequent cycles to serve as template for the target full-length product. The process parameters such as primer ratio, elongation temperature and cycle numbers were optimized. In addition, the fusion products produced with this method were successfully applied in seamless genome editing. The ...


Plants As Biofactories To Produce Mammalian Tumor Suppressor Micrornas, John Lachlan MacArthur 2018 University of South Carolina

Plants As Biofactories To Produce Mammalian Tumor Suppressor Micrornas, John Lachlan Macarthur

Theses and Dissertations

MicroRNAs (miRNAs) are small noncoding single stranded RNAs that are considered master regulators of gene expression. They are also an emerging class of therapeutic agents with significant potential for the prevention and treatment of many diseases, including cancer. Many different forms of cancer are associated with loss or reduced accumulation of one or more miRNAs that function as tumor suppressors. In animal models, restoration of missing tumor suppressor miRNAs prevents the initiation, progression and/or spread of the disease. However, the current absence of an efficient method for delivery of therapeutic miRNAs is a critical barrier to their use. The ...


Molded Features In Pdms For Fabricating Bacterial Cellulose For Various Geometries, Mitchell Habegger 2018 The University of Akron

Molded Features In Pdms For Fabricating Bacterial Cellulose For Various Geometries, Mitchell Habegger

Honors Research Projects

The purpose of producing features on bacterial cellulose (BC) is to facilitate the elongation and alignment for cells, in this case Normal Human Dermal Fibroblast (NHDF) cells. The elongated cells have applications in wound healing, tissue engineering, disease diagnostics, and many other fields. Experiments were run to test the effectiveness of transferring features to BC sheets from features induced by fracturing on polydimethylsiloxane (PDMS) and those duplicated from molds with existing features. The features were duplicated to BC sheets by either air drying or Guided Assembly-Based Biolithography (GAB). The research results showed that fracture inducing on PDMS produced very small ...


The Effects Of Emerging Technology On Healthcare And The Difficulties Of Integration, Skyler J. Pavlish-Carpenter 2018 University of Central Florida

The Effects Of Emerging Technology On Healthcare And The Difficulties Of Integration, Skyler J. Pavlish-Carpenter

Honors in the Major Theses

Background: Disruptive technology describes technology that is significantly more advanced than previous iterations, such as: 3D printing, genetic manipulation, stem cell research, innovative surgical procedures, and computer-based charting software. These technologies often require extensive overhauls to implement into older systems and must overcome many difficult financial and societal complications before they can be widely used. In a field like healthcare that makes frequent advancements, these difficulties can mean that the technology will not be utilized to its full potential or implemented at all.

Objective: To determine the inhibiting factors that prevent disruptive technology from being implemented in conventional healthcare.

Methods ...


A Collaborative Solution To Harmful Algal Blooms In Utah, Kyle Hillman, Bethany Jensen, Ammon Balle 2018 Utah State University

A Collaborative Solution To Harmful Algal Blooms In Utah, Kyle Hillman, Bethany Jensen, Ammon Balle

Research on Capitol Hill

Harmful algal blooms (HABs)…

  • affect Utah Lake, Scofield Reservoir, Jordanelle Reservoir, Mantua Lake, and other water bodies throughout Utah
  • are toxic to public health, the environment, and the economy


Exogenous Fniii 12-14 Regulates Tgf-Β1-Induced Markers, Hilmi M. Humeid 2018 Virginia Commonwealth University

Exogenous Fniii 12-14 Regulates Tgf-Β1-Induced Markers, Hilmi M. Humeid

Theses and Dissertations

The extracellular matrix protein Fibronectin (FN) plays an important role in cell contractility, differentiation, growth, adhesion, and migration. The 12th -14th Type III repeats of FN (FNIII 12-14), also referred to as the Heparin-II domain, comprise a highly promiscuous growth factor (GF) binding region. This binding domain aids in cellular signaling initiated from the ECM. Additionally, FN has the ability to assemble into fibrils under certain conditions, mostly observed during cell contractile processes such as those that initiate due to upregulation of Transforming Growth Factor Beta 1 (TGF-β1) [1], [2]. Previous work from our lab has shown that self-assembly of ...


The Role Of Protein-Protein Interactions In Inducing Interfacial Aggregation, Lea Line Gisele Sorret 2018 University of Colorado at Boulder

The Role Of Protein-Protein Interactions In Inducing Interfacial Aggregation, Lea Line Gisele Sorret

Chemical & Biological Engineering Graduate Theses & Dissertations

The synergic exposure to silicone oil-water interfaces and to agitation has been shown to promote the aggregation of therapeutic proteins. Silicone oil is typically used as a lubricant for manufacturing surfaces and pharmaceutical containers such as glass prefilled syringes. This is a problem as protein aggregates formed at the silicone oil-water interface may shed into bulk solutions, leading to product recalls or adverse immunogenicity upon injections into patients.

Protein aggregation in solution occurs as a result of attractive protein-protein interactions (PPI). PPI can be characterized using techniques such as dynamic light scattering which measures the dependence of molecular diffusivity on ...


Computer-Aided Diagnoses (Cad) System: An Artificial Neural Network Approach To Mri Analysis And Diagnosis Of Alzheimer's Disease (Ad), Berizohar Padilla Cerezo 2017 California Polytechnic State University, San Luis Obispo

Computer-Aided Diagnoses (Cad) System: An Artificial Neural Network Approach To Mri Analysis And Diagnosis Of Alzheimer's Disease (Ad), Berizohar Padilla Cerezo

Master's Theses and Project Reports

Alzheimer’s disease (AD) is a chronic and progressive, irreversible syndrome that deteriorates the cognitive functions. Official death certificates of 2013 reported 84,767 deaths from Alzheimer’s disease, making it the 6th leading cause of death in the United States. The rate of AD is estimated to double by 2050. The neurodegeneration of AD occurs decades before symptoms of dementia are evident. Therefore, having an efficient methodology for the early and proper diagnosis can lead to more effective treatments.

Neuroimaging techniques such as magnetic resonance imaging (MRI) can detect changes in the brain of living subjects. Moreover, medical imaging ...


Implantation Of Nanocellulose In The Zebrafish Model, Hanna J. Anderson 2017 University of Maine

Implantation Of Nanocellulose In The Zebrafish Model, Hanna J. Anderson

Honors College

The number of novel materials for use in biomedical implantation is expanding rapidly, increasing the success rates of implant procedures. Nanocellulose is being assessed as a sustainable and biocompatible material, offering an alternative to conventional polymer or metal designs with the appropriate structure for potential tissue integration. In this research, the capacity of cellulose nanofibers to serve as biomedical implants is assessed through examination of immune responses of transgenic zebrafish, utilizing bright field and confocal fluorescence microscopy. Methods for creating microincisions for the implantation of dense cellulose nanofiber shards in the zebrafish model were explored, and a surgical protocol was ...


Investigation And Engineering Of Polyketide Biosynthetic Pathways, Lei Sun 2017 Utah State University

Investigation And Engineering Of Polyketide Biosynthetic Pathways, Lei Sun

All Graduate Theses and Dissertations

This research is focused on investigation and engineering of natural product biosynthetic pathways for efficient production of pharmaceutically important molecules or generation of new bioactive molecules for drug development.

Natural products are an important source of therapeutics, such as chromomycin (anti-cancer), emodin (anti-inflammatory and anti-tumor) and sprolaxine (anti-Helicobacter pylori). Metabolic engineering of natural product biosynthetic pathways shows its promise for creating and producing valuable compounds with chemical diversity for drug discovery. One goal of this research is to create highly efficient strains to biosynthesize valuable natural products. The engineered Streptomyces roseiscleroticus strain constructed in this work showed higher titers of ...


Gender And Participation In An Engineering Problem-Based Learning Environment, Laura Hirshfield, Milo D. Koretsky 2017 University of Michigan

Gender And Participation In An Engineering Problem-Based Learning Environment, Laura Hirshfield, Milo D. Koretsky

Interdisciplinary Journal of Problem-Based Learning

The use of problem-based learning (PBL) is gaining attention in the engineering classroom as a way to help students synthesize foundational knowledge and to better prepare students for practice. In this work, we study the discourse interactions between 27 student teams and two instructors in an engineering PBL environment to analyze how participation is distributed among team members, paying particular attention to the differences between male and female students. There were no statistically significant differences between the amount that male and female students spoke; however, stereotypical gender roles and traditional gendered behavior did manifest in the discussion. Also, regardless of ...


Escherichia Coli Attachment To Model Particulates: The Effects Of Bacterial Cell Characteristics And Particulate Properties, Xiao Liang, Chunyu Liao, Michelle L. Soupir, Laura R. Jarboe, Michael L. Thompson, Philip M. Dixon 2017 Iowa State University

Escherichia Coli Attachment To Model Particulates: The Effects Of Bacterial Cell Characteristics And Particulate Properties, Xiao Liang, Chunyu Liao, Michelle L. Soupir, Laura R. Jarboe, Michael L. Thompson, Philip M. Dixon

Chemical and Biological Engineering Publications

E. coli bacteria move in streams freely in a planktonic state or attached to suspended particulates. Attachment is a dynamic process, and the fraction of attached microorganisms is thought to be affected by both bacterial characteristics and particulate properties. In this study, we investigated how the properties of cell surfaces and stream particulates influence attachment. Attachment assays were conducted for 77 E. coli strains and three model particulates (ferrihydrite, Ca-montmorillonite, or corn stover) under environmentally relevant conditions. Surface area, particle size distribution, and total carbon content were determined for each type of particulate. Among the three particulates, attachment fractions to ...


Influence Of Fibroblasts On Functional Arteriogenesis In A Murine Chronic Hindlimb Ischemia Model, Ashli A. Santos 2017 California Polytechnic State University, San Luis Obispo

Influence Of Fibroblasts On Functional Arteriogenesis In A Murine Chronic Hindlimb Ischemia Model, Ashli A. Santos

Biomedical Eng/General Eng.

Peripheral arterial occlusive disease (PAOD) occurs when there is a narrowing or blockage – usually a buildup of plaque - within the arteries that reduces blood flow to tissues which can chronic ischemia. As with most diseases, early detection and proactive treatment are important to maximize prognosis. Exercise effectively treats PAOD, but due to ischemic pain in the limbs, or intermittent claudication (IC), exercise can become painful and difficult. Due to the buildup of plaque, occlusions create an ischemic environment that changes the pressure distribution in collateral networks and increases the shear stress in transverse collaterals. Those two responses signal the beginning ...


The Subject Librarian Newsletter, Engineering And Computer Science, Fall 2017, Buenaventura "Ven" Basco 2017 University of Central Florida

The Subject Librarian Newsletter, Engineering And Computer Science, Fall 2017, Buenaventura "Ven" Basco

Libraries' Newsletters

No abstract provided.


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