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Bioimaging and Biomedical Optics Commons

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Pilot Study To Assess Breathing During Sight-Read Stringed Instrument Performance, Collin T. Erickson, Clark Potter MM, MFA, Gregory Bashford Ph.D 2016 University of Nebraska-Lincoln

Pilot Study To Assess Breathing During Sight-Read Stringed Instrument Performance, Collin T. Erickson, Clark Potter Mm, Mfa, Gregory Bashford Ph.D

UCARE Research Products

For many musicians, one common occurrence during a performance is the presence of stage fright. Stage fright, though not always expressed can influence the confidence and self-esteem of a performer, and thus can affect the quality of the performance. Every day musicians are affected by stage fright, and there has been no exact solution as to how to lessen the feeling of anxiety musicians feel before performing. No data has been collected to find the correlation between the regularity of breathing and the level of stage fright that a person feels during their performance. A way to quantify regularity of ...


Adaptive Kernel Estimation For Enhanced Filtering And Pattern Classification Of Magnetic Resonance Imaging: Novel Techniques For Evaluating The Biomechanics And Pathologic Conditions Of The Lumbar Spine, Nicholas Vincent Battaglia 2016 University of Tennessee - Knoxville

Adaptive Kernel Estimation For Enhanced Filtering And Pattern Classification Of Magnetic Resonance Imaging: Novel Techniques For Evaluating The Biomechanics And Pathologic Conditions Of The Lumbar Spine, Nicholas Vincent Battaglia

Doctoral Dissertations

This dissertation investigates the contribution the lumbar spine musculature has on etiological and pathogenic characteristics of low back pain and lumbar spondylosis. This endeavor necessarily required a two-step process: 1) design of an accurate post-processing method for extracting relevant information via magnetic resonance images and 2) determine pathological trends by elucidating high-dimensional datasets through multivariate pattern classification. The lumbar musculature was initially evaluated by post-processing and segmentation of magnetic resonance (MR) images of the lumbar spine, which characteristically suffer from nonlinear corruption of the signal intensity. This so called intensity inhomogeneity degrades the efficacy of traditional intensity-based segmentation algorithms. Proposed ...


The Effectiveness, Efficiency, And Appeal Of Pediatric Echocardiography Protocol Training For First Year Pediatric Cardiology Fellows, Lynne M. Brown 2016 University of Wisconsin-Milwaukee

The Effectiveness, Efficiency, And Appeal Of Pediatric Echocardiography Protocol Training For First Year Pediatric Cardiology Fellows, Lynne M. Brown

Theses and Dissertations

Echocardiography training for pediatric cardiology fellows is complex and academic hospitals strive to provide high-quality training using limited resources. The purpose of this embedded single case study design was to evaluate the effectiveness, efficiency, and appeal of a newly developed 10-day echocardiography protocol learning module for first year pediatric cardiology fellows. Using blended learning methods that included didactic lectures, online learning activities, and interactive games, the learning module was the first step in the process of training pediatric cardiology fellows to perform echocardiograms independently with limited supervision during their first year of fellowship. At the end of the 10-day module ...


Design Of A 3d-Printed Device For Diffuse Reflectance Spectroscopy Calibration As A Step Towards Clinical Translation, Nontapoth Vongkittiargorn 2016 University of Arkansas, Fayetteville

Design Of A 3d-Printed Device For Diffuse Reflectance Spectroscopy Calibration As A Step Towards Clinical Translation, Nontapoth Vongkittiargorn

Biomedical Engineering Undergraduate Honors Theses

The diffusive reflectance and spectroscopic microendoscopy (DRSME) is a multimodal imaging system that harnesses its usefulness from different light sources. One of the modalities, diffuse reflectance spectroscopy (DRS), has been used in our lab to investigate optical properties of epithelial tissues using a broadband white light as the main source. Calibration of DRS is required as it can obtain the maximum intensity and convert it to absolute reflectance. Current manual-adjusted calibration can be lengthy and often lead to inconsistent results. Therefore, a new method of calibration is introduced where additive manufacturing (or 3D printing) technology is fully utilized.

The instrumentation ...


Atomic Force Microscopy Based Dna Analysis, Drew Creighton 2016 University of Arkansas, Fayetteville

Atomic Force Microscopy Based Dna Analysis, Drew Creighton

Mechanical Engineering Undergraduate Honors Theses

This report explores dry and wet scanning of a surface and DNA pickup using an AFM, as well as fluorescent staining of DNA. Dry and wet scans of DNA were obtained using a cantilever AFM tip in tapping mode. Dry scans were found to be clearer than wet scans; however, the drying process was found to decrease the thickness of DNA 2–4 times less than its original thickness. Alternately, wet scans were found to be less clear than dry scans and introduced more noise into the images obtained. Additionally, DNA kept its initial thickness during wet scanning. DNA was ...


Optimized Protocol For Measuring 2-Nbdg Uptake As A Cellular Marker Of Glycolytic Demand, Drew Folgmann 2016 University of Arkansas, Fayetteville

Optimized Protocol For Measuring 2-Nbdg Uptake As A Cellular Marker Of Glycolytic Demand, Drew Folgmann

Biomedical Engineering Undergraduate Honors Theses

2[N-(7-nitrobenz-2-oxa-1,2-diaxol-4-yl)amino]-2-deoxyglucose (2-NBDG) a fluorescently tagged analog of FDG is ideal for in vitro studies and imaging. 2-NBDG has been proven to be an adequate marker for glucose uptake in many different types of cells [1]. However, across the spectrum of 2-NBDG use a lack of consensus is observed for the following questions. What is the ideal time to fast cells to optimize cellular uptake of 2-NBDG? Also, what is the ideal concentration of 2-NBDG to be used when quantifying glucose uptake? Finally, what is the effect of serum on the uptake of 2-NBDG? To answer ...


Intravital Microscopy Of Tumor Oxygenation And Glycolytic Demand, Jesse D. Ivers 2016 University of Arkansas, Fayetteville

Intravital Microscopy Of Tumor Oxygenation And Glycolytic Demand, Jesse D. Ivers

Biomedical Engineering Undergraduate Honors Theses

There is growing concern about the over treatment of cancer because treatments are based primarily on tumor anatomy. In this study, we aim to begin the process of addressing that issue by developing an intravital technique for optically analyzing tumor biology. Two traits were identified as having significant importance in the aggression of a tumor, vascular oxygenation (SO2) and glycolytic demand. Dorsal skin flap window chambers were implanted and 4T1 and 67NR cancer cells were injected to provide a tumor model for the development of this intravital quantification technique. This study provides a detailed protocol from instrumentation setup to surgical ...


Multiphoton Imaging Of Labeled Breast Cancer Cells To Quantify Intra And Extracellular Receptors, Sydney C. Wiggins 2016 University of Arkansas, Fayetteville

Multiphoton Imaging Of Labeled Breast Cancer Cells To Quantify Intra And Extracellular Receptors, Sydney C. Wiggins

Biomedical Engineering Undergraduate Honors Theses

Every year 200,000 women in the United States are diagnosed with breast cancer. Of the cases diagnosed, 10% -15% are classified as triple negative breast cancer (TNBC) due to the absence of estrogen, progesterone, and HER-2/Neu receptors. This breast cancer sub-type is markedly more aggressive and twice as likely to develop in premenopausal women. TNBC is resistant to endocrine therapies and current targeted agents, making clinical need for the development of validated therapeutics for TNBC a pressing matter. To initiate drug development, the internalization of directly immunolabeled epidermal growth factor receptors (EGFR) in SK-BR-3 human breast adenocarcinoma cells ...


Determining The Effect Of Delivery Rate On Glucose Uptake By Cancer Cells, Luke Felton 2016 University of Arkansas, Fayetteville

Determining The Effect Of Delivery Rate On Glucose Uptake By Cancer Cells, Luke Felton

Biomedical Engineering Undergraduate Honors Theses

Radiation therapy is one of the most commonly performed cancer treatment therapies today. Radiation therapy can cause can changes to tumor vasculature and affect blood flow rates. It is important to determine how changes in blood flow rates affect tumor glycolytic uptake to better understand tumor response to radiation therapy (1). The goal of this study was to use a fluorescent analog of glucose, 2-NBDG, to study the effect of varying flow rates on glucose uptake. A microfluidic device was constructed to flow 2-NBDG through. A murine metastatic breast cancer cell line was then cultured inside this microfluidic channel, and ...


Lesion Identification And The Effect Of Lesion On Motor Mapping After Stroke, Ruixi Zhou 2016 Washington University in St. Louis

Lesion Identification And The Effect Of Lesion On Motor Mapping After Stroke, Ruixi Zhou

Engineering and Applied Science Theses & Dissertations

Stroke is the most common cause of long-term severe disability and the motor system that is most commonly affected in stroke. One of the mechanisms that underlies recovery of motor deficits is reorganization or remapping of functional representations around the motor cortex. This mechanism has been shown in monkeys, but results in human subjects have been variable. In this thesis, I used a database that includes longitudinal behavioral and multimodal imaging data in both stroke patients and healthy controls for two research projects. Firstly, I improved an automatic lesion segmentation method to aid in the identification of the location and ...


Experimental Assessment Of Mouse Sociability Using An Automated Image Processing Approach, Frency Varghese, Jessica A. Burket, Andrew D. Benson, Stephen I. Deutsch, Christian W. Zemlin 2016 Old Dominion University

Experimental Assessment Of Mouse Sociability Using An Automated Image Processing Approach, Frency Varghese, Jessica A. Burket, Andrew D. Benson, Stephen I. Deutsch, Christian W. Zemlin

Electrical & Computer Engineering Faculty Publications

Mouse is the preferred model organism for testing drugs designed to increase sociability. We present a method to quantify mouse sociability in which the test mouse is placed in a standardized apparatus and relevant behaviors are assessed in three different sessions (called session I, II, and III). The apparatus has three compartments (see Figure 1), the left and right compartments contain an inverted cup which can house a mouse (called “stimulus mouse”). In session I, the test mouse is placed in the cage and its mobility is characterized by the number of transitions made between compartments. In session II, a ...


The Connectivity Domain: Analyzing Resting State Fmri Data Using Feature-Based Data-Driven And Model-Based Methods, Armin Iraji, Vince D. Calhoun, Natalie M. Wiseman, Esmaeil Davoodi-Bojd, Mohammad R. N. Avanaki, Zhifeng Kou 2016 Wayne State University

The Connectivity Domain: Analyzing Resting State Fmri Data Using Feature-Based Data-Driven And Model-Based Methods, Armin Iraji, Vince D. Calhoun, Natalie M. Wiseman, Esmaeil Davoodi-Bojd, Mohammad R. N. Avanaki, Zhifeng Kou

Biomedical Engineering Faculty Research Publications

Spontaneous fluctuations of resting state functional MRI (rsfMRI) have been widely used to understand the macro-connectome of the human brain. However, these fluctuations are not synchronized among subjects, which leads to limitations and makes utilization of first-level model-based methods challenging. Considering this limitation of rsfMRI data in the time domain, we propose to transfer the spatiotemporal information of the rsfMRI data to another domain, the connectivity domain, in which each value represents the same effect across subjects. Using a set of seed networks and a connectivity index to calculate the functional connectivity for each seed network, we transform data into ...


Structural-Functional Brain Connectivity Underlying Integrative Sensorimotor Function After Stroke, Benjamin Thomas Kalinosky 2016 Marquette University

Structural-Functional Brain Connectivity Underlying Integrative Sensorimotor Function After Stroke, Benjamin Thomas Kalinosky

Dissertations (2009 -)

In this dissertation research project, we demonstrated the relationship between the structural and functional connections across the brain in stroke survivors. We used this information to predict arm function in stroke survivors, suggesting that the tools developed through this research will be useful for prescribing individualized rehabilitation strategies in people after stroke. Current clinical methods for rehabilitating sensorimotor function after stroke are not based on the locus of injury in the brain. Instead, therapies are generalized, treating symptoms such as weakness and spasticity. This results in outcomes that are highly variable, with severity of impairment immediately following stroke as the ...


Injury Risk Assessment Of The Femur In Children With Osteogenesis Imperfecta, Jessica Marie Fritz 2016 Marquette University

Injury Risk Assessment Of The Femur In Children With Osteogenesis Imperfecta, Jessica Marie Fritz

Dissertations (2009 -)

Osteogenesis imperfecta (OI) is a genetic disorder characterized by increased bone fragility and decreased bone mass, which leads to high rates of bone fracture. OI has a prevalence of 1/5,000 to 1/10,000 in the United States. About 90% of persons with OI have a genetic mutation in the coding for collagen type I, which is the major protein of connective tissues, including bone. While its prevalence classifies it as a rare disease, it is the most common disorder of bone etiology. Until recently, little was known about the mechanics and materials of OI bone or their ...


Optimization-Based Interactive Segmentation Interface For Multiregion Problems., John S H Baxter, Martin Rajchl, Terry M Peters, Elvis C S Chen 2016 Western University

Optimization-Based Interactive Segmentation Interface For Multiregion Problems., John S H Baxter, Martin Rajchl, Terry M Peters, Elvis C S Chen

Robarts Imaging Publications

Interactive segmentation is becoming of increasing interest to the medical imaging community in that it combines the positive aspects of both manual and automated segmentation. However, general-purpose tools have been lacking in terms of segmenting multiple regions simultaneously with a high degree of coupling between groups of labels. Hierarchical max-flow segmentation has taken advantage of this coupling for individual applications, but until recently, these algorithms were constrained to a particular hierarchy and could not be considered general-purpose. In a generalized form, the hierarchy for any given segmentation problem is specified in run-time, allowing different hierarchies to be quickly explored. We ...


Cerebrovascular Reactivity Change With Increased Intracranial Pressure, Madison Burger, M. Alwatban, Allison Porter, Ben Hage, Greg Bashford 2016 University of Nebraska-Lincoln

Cerebrovascular Reactivity Change With Increased Intracranial Pressure, Madison Burger, M. Alwatban, Allison Porter, Ben Hage, Greg Bashford

UCARE Research Products

Cerebrovascular reactivity (CVR) is an important regulatory factor of the brain. The parameter controls the dilation and constriction of blood vessels in the brain in response to certain stimuli, such as carbon dioxide. Abnormal CVR values could potentially be indicators of poorly functioning regulatory systems. Testing of CVR is one method of assessing the brain's regulatory capabilities. The purpose of this study was to test for a relationship between CVR and intracranial pressure (ICP).

In this study, increased intracranial pressure was created in 4 female subjects through head down tilt, using an inversion table. Subjects were lowered in the ...


Analysis Of Breath-Holding Index As An Assessment Of Cerebrovascular Reactivity, Allison P. Porter, Madison Burger, Mohammed Alwatban, Benjamin Hage, Greg Bashford 2016 University of Nebraska - Lincoln

Analysis Of Breath-Holding Index As An Assessment Of Cerebrovascular Reactivity, Allison P. Porter, Madison Burger, Mohammed Alwatban, Benjamin Hage, Greg Bashford

UCARE Research Products

Cerebrovascular reactivity (CVR) is a key factor in regulating blood flow into the brain, and a marker for vascular disease. If the brain's regulatory system is not working, a patient may be in serious trouble. Testing of CVR is one method of assessing the brain's regulatory capabilities. Transcranial Doppler ultrasound (TCD) is one tool to measure CVR. In this method, carbon dioxide in the blood is transiently increased (such as with the holding of breath), and the resulting blood flow in the brain is measured. In this study, we are going to measure the variability of the breathholding ...


Circle Of Willis Model For Transcranial Doppler Ultrasound Training, Conner J. Beyersdorf, Ben Hage, Greg Bashford 2016 University of Nebraska-Lincoln

Circle Of Willis Model For Transcranial Doppler Ultrasound Training, Conner J. Beyersdorf, Ben Hage, Greg Bashford

UCARE Research Products

Transcranial Doppler (TCD) ultrasound is a technique involving the use of high frequency transmitters to measure intracranial blood flow. The brain is supplied by blood in an arterial anastomosis called the Circle of Willis. Using TCD ultrasound on the circle is difficult and requires practice and teaching. A functional model of the Circle of Willis could prove to be a valuable teaching tool. Through the use of AutoCAD and 3D printing software, an anatomically accurate model was created and set in gelatin phantom inside of a plastic skull. Milk was pumped through the model with a peristaltic pump to simulate ...


Increasing 18f-Fdg Pet/Ct Capabilities In Radiotherapy For Lung And Esophageal Cancer Via Image Feature Analysis, Jasmine Alexandria Oliver 2016 University of South Florida

Increasing 18f-Fdg Pet/Ct Capabilities In Radiotherapy For Lung And Esophageal Cancer Via Image Feature Analysis, Jasmine Alexandria Oliver

Graduate Theses and Dissertations

Positron Emission Tomography (PET) is an imaging modality that has become increasingly beneficial in Radiotherapy by improving treatment planning (1). PET reveals tumor volumes that are not well visualized on computed tomography CT or MRI, recognizes metastatic disease, and assesses radiotherapy treatment (1). It also reveals areas of the tumor that are more radiosensitive allowing for dose painting - a non-homogenous dose treatment across the tumor (1). However, PET is not without limitations. The quantitative unit of PET images, the Standardized Uptake Value (SUV), is affected by many factors such as reconstruction algorithm, patient weight, and tracer uptake time (2). In ...


Europium(Ii)-Containing Complexes For Responsive Magnetic Resonance Imaging, Levi Alexander Ekanger 2016 Wayne State University

Europium(Ii)-Containing Complexes For Responsive Magnetic Resonance Imaging, Levi Alexander Ekanger

Wayne State University Dissertations

The research projects described in this dissertation are focused on studying the oxidation of EuII-containing complexes within the context of responsive MRI. Prior to this research, EuII had not been explored within the context of oxidation-responsive MRI nor had the use of this ion been reported in vivo. The results of these studies enable predictions about the oxidation-responsive behavior of EuII-containing complexes in vitro and in vivo.

The EuII-containing cryptate 1.33 was used to evaluate EuII-based positive contrast enhancement after intravenous, intraperitoneal, and subcutaneous injections. The transitory behavior of the positive contrast enhancement correlated with reported levels of oxygenation ...


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